You are told that a sample has between 2.5 X 10^3 and 2.5 X 10^5 cells/mL. Devise a complete but efficient (that is, no extra plates!) dilution scheme that will ensure getting a countable plate.
To devise a complete but efficient dilution scheme for this sample, we can start by assuming that the actual number of cells in the sample is closer to the higher end of the range, 2.5 X 10^5 cells/mL. We can then calculate the dilution factor needed to get a countable plate by using the following formula:
dilution factor = (desired plate count) / (actual plate count)
Assuming that a countable plate has between 30-300 colonies, we can aim for a plate count of 150 colonies, which is the midpoint of this range. Using this information, the dilution factor needed would be:
dilution factor = 150 / (2.5 X 10^5) = 6 X 10^-4
To achieve this dilution factor, we can perform a series of 3 dilutions:
1. Take 0.5 mL of the original sample and add it to 499.5 mL of sterile diluent (such as saline or broth). This is a 1:1000 dilution.
2. Take 0.5 mL of the 1:1000 dilution and add it to 499.5 mL of sterile diluent. This is a 1:1000 dilution of the first dilution, resulting in a 1:1,000,000 dilution of the original sample.
3. Take 0.6 mL of the 1:1,000,000 dilution and add it to 4.4 mL of sterile diluent. This is a 1:10 dilution of the second dilution, resulting in a final dilution of 1:10,000,000.
Plating 0.1 mL of the final dilution on an agar plate and incubating it for the appropriate time period should result in a countable number of colonies, assuming the initial estimate of 2.5 X 10^5 cells/mL is accurate.
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you have studied the histological structure of a number of organs in this laboratory. three of these are diagrammed below. identify and correctly label each. review sheet 38
The first diagram is of the human organs chart is of the lung, The second diagram is of the stomach and The third diagram is of the kidney.
Here is a general guide to identifying and labeling histological structures:1. Identify the tissue type: Determine whether the tissue is epithelial, connective, muscular, or nervous. Look at the arrangement of cells, the type of cells present, and the presence of any extracellular matrix.2. Identify the organ: Once you have identified the tissue type, you can then identify the organ it belongs to by looking at the overall shape, structure, and location of the tissue.3. Label the parts.
Finally, label the specific parts of the organ, such as the lumen, basement membrane, glands, or blood vessels. Here are some commonly studied organs in histology, along with their identifying features: Organ: Stomach, Tissue type: Simple columnar epithelium, Location: Upper left quadrant of the abdomen, Features: Rugae, gastric pits, glands, muscularis mucosae, submucosa Organ: Liver, Tissue type: Parenchymal and stromal cells
Location: Upper right quadrant of the abdomen, Features: Hepatocytes, sinusoids, bile canaliculi, central vein, portal triad, Organ: Kidney, Tissue type: Nephron, Location: Abdominal cavity, Features: Glomerulus, Bowman's capsule, proximal and distal convoluted tubules, collecting duct, renal pelvis, ureter.
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Two flasks each contain a clear liquid . The liquid in Flask A turns blue when shaken . The liquid in Flask B does not . What would be the next logical step in determining if the liquids are the same or different .
aAnswer:
aa
Explanation:
Which of the following nutrients is provided by the agar Ingredient that is added to solidify growth media like trypticase soy agar? excellent source of nitrogen source of phosphorous energy source none of these choices
The agar ingredient that is added to solidify growth media like trypticase soy agar does not provide any of the mentioned nutrients. Therefore, the answer to your question is "none of these choices."
Agar is a gelatinous substance that is derived from seaweed and is commonly used as a thickener and stabilizer in food and pharmaceutical products. Agar is a polysaccharide that is composed of agarose and agaropectin and is often used as substitute for gelatin in vegan and vegetarian recipes.
Agar is the most commonly used in food industry to thicken and stabilize products like ice cream, jelly, and canned soup
However, it is important to note that agar does provide a stable and solid surface for the bacterial growth and replication, thus making it a valuable component in microbiology research.
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What is a prokaryotic cell? Help please
Answer:
A cell whose genetic material isn't found in a membrane bound nucleus.
Explanation:
according to the ten percent law, only about 10% of the energy consumed makes it from one trophic level to the next. with this in mind, if you started with 1,000 calories of willow leaves, how many calories would make it to each of these other animals?
About 10% of the energy in the food is fixed into animal flesh.
Food is any nutrient-wealthy fabric consumed or absorbed by means of human beings, animals, or flora in an effort to sustain existence and increase.
Nutrients are materials that provide: strength for activity, growth, and all capabilities of the frame together with respiration, digesting meals, and preserving heat; substances for the boom and restoration of the body, and for retaining the immune device wholesome.
Eating conduct is defined as “conscious, collective, and repetitive behaviors, which lead human beings to pick, consume, and use certain ingredients or diets, in reaction to social and cultural influences.
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Potassium atoms have 19 protons and electrons. Most potassium atoms contain 20 neutrons. How would the potassium atom change if another atom took away one of the potassium atom's electrons?
The potassium atom would become a negative ion with a charge of -1.
None of these choices are correct.
The potassium atom would become a potassium ion with a charge of +1.
The mass number of potassium would change from 39 to 38.
Answer:
The potassium atom would become a negative ion with a charge of -1.
Explanation:
If another atom took away one of the potassium atom's electrons, the potassium atom would have one more proton than electron, resulting in a net positive charge. The atom would become a negative ion, also known as an anion, with a charge of -1.
Which statement describes the motion of Earth around the sun?
Answer:
The speed of Earth is constant regardless of its distance from the sun. The speed of Earth is constant because the distance remains the same between Earth and the sun.
The speed of Earth is constant regardless of its distance from the sun.
What does Hyperbole mean
Answer:
Hyperbole is an exaggerated statement(s) or claim(s) not meant to be taken literally.
Explanation: Hope this helps:)
Hyperbole means that something is being exaggerated such as someone saying that the ice cream is a mile high.
Or sometimes in MY case, when my mum is shouting at one of us I sometimes say she is acting like a bear. (That usually get a laugh or two outta someone in the house )
Hope this helped you out mate, and if it didn't then I'm sorry for wasting time.
How many calories should I eat to gain 0.5 pounds of muscle a week I'm 73 pounds and 4 foot 5 at 13
Making Comparisons Read the definition of parasites and predators, and then explain how parasites differ from predators.
Answer:
Parasites only wish to suck the life out of their prey, but predators wish to kill and eat their prey. The main difference is killing what they eat.
based on your analysis of the headlines, which problem, highlighted by the covid-19 pandemic, is being referenced when the journalists are talking about a digital divide?
Answer:
Disparities in internet access between students of different economic groups.
Explanation: confía :)
Develop an argument from evidence on how human activity can lead to ocean acidification? Further explain what we can do, as humans, to decrease the amount of ocean acidification occurring in aquatic environments.
Answer
Ocean acidification results from the solubility of CO2 in oceans increasing the H+ concentrations, and depletion of bicarbonates concentrations.
Therefore, human activities which increases the concentration of CO2 in the atmosphere, will raise the atmospheric CO2 concentration. CO2 is constantly being absorbed by green plants maintaining its concentration through carbon cycle,Therefore massive deforestation which removes green plants, cutting down photosynthesis is one of human activities which raises CO2 levels of the atmosphere.The CO2 dissolves in the oceans to raise its acidic levels.Lower pH.
Another source is fossil fuels emissions which add more CO2 to the atmosphere.
Explanation:
The current population of a threatened animal species is 1.6 million, but it is declining with a half-life of 20 years. How many animals will be left in 40 years? in 65 years? The population after 40 years will be animals. (Round to the nearest whole number as needed.) The population after 65 years will be animals. (Round to the nearest whole number as needed.)
The population after 40 years will be 400,000 animals, and the population after 65 years will be approximately 336,000 animals.
To determine the population after a certain time period, we can use the formula for exponential decay;
N(t) = N0 × \((1/2)^{(t/T)}\)
Where;
N(t) is the population at time t
N0 is the initial population
t is the time elapsed
T is the half-life of the population
Given;
N0 = 1.6 million
T = 20 years
Let's calculate the population after 40 years;
N(40) = 1.6 million × \(1/2^{(65/20)}\)
= 1.6 million × (1/2)²
= 1.6 million × (1/4)
= 400,000
Therefore, the population after 40 years will be 400,000 animals.
Now, let's calculate the population after 65 years;
N(65) = 1.6 million × \((1/2)^{(65/20)}\)
= 1.6 million × \((1/2)^{(13/4)}\)
≈ 1.6 million × 0.210
≈ 336,000
Therefore, the population after 65 years will be approximately 336,000 animals.
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2H2 + O2 + 2H2O
In this chemical equation, there are two Hz molecules and
Oz molecule
present in the reactants (the
_). The arrow represents the reaction
occurring. After the reaction, two water
are produced
It is important to point out that during a chemical reaction, the amount of an element
present in the reactants will be
to the amount in the products.
For example, if you start with two oxygen atoms, you will end with
oxygen
atoms. Similarly, if you start w 4g of hydrogen, you will
4g of
hydrogen. The total mass of materials
the total mass of products.
This is known as the Law of
of
During
a reaction, matter can neither be
nor
only
changed in form.
Broken
Equations
left
Chemicals
equal
matter
produced
Conservation
equals
mixtures
produce
created
formed
molecules
reactions
destroyed
ingredients
new
reacting
one
two
Answer:
Hydrogen peroxide is the best way to use it
Explanation:
Sheets composed of two layers of amphipathic molecules arranged with the hydrophilic groups on the surface and the hydrophobic groups buried in the center that form in water are called A) micelles. B) liposomes. C) vacuoles. D) bilayer membranes. E) none of the above
Answer:
D) bilayer membranes
Explanation:
Two lipidic bilayers compose the cell membrane. There are also proteins and glucans incrusted in between. Lipids are amphipathic molecules with hydrophilic heads -negatively charged phosphate group- and hydrophobic tails. Lipids are arranged with their hydrophilic polar heads facing the exterior and the interior of the cells, while their hydrophobic tails are against each other, constituting the internal part of the membrane. Membranes are fluid, which means that the composing molecules can move through them.
Lipids can easily change places with other neighboring lipids by lateral diffusion in the same layer. This is passive diffusion, which means that it does not need energy to happen.
Lipids can also diffuse transversally to the other layer but not as easily as lateral diffusion. Jumps between monolayers are infrequent as the lipidic polar heads meet the fatty acid barrier.
There are also other lipidic movements as rotational diffusion that imply the rotation of the molecule.
how can so much DNA could fit inside a bacterial cell.
Answer:
The reason so much DNA could fit inside a bacterial is because the DNA are double-stranded, looped, coiled, and foleed into the cell. The DNA is tightly fitted around the nucleus.
Explanation:
Have a great summer :)
mot nucleoxom co cau truc gom
You are requested to implement a simple virus management system. "A virus is a submicroscopic infectious agent that replicates only inside the fiving cells of an organism. Viruses infect all life forms, from animals and plants to microorganisms, including bacteria and archaea. "Viruses can be classified into various categories according to the Baltimore classitication (see link for more details). Any virus can be identified by many fields including an official name, date when it was first discovered, who discovered... 1- (1 mark) Describe the virus and research Lab data types, Virus and ResearchLab, using Java classes. Make sure to use Java inheritance, an interface, and an abstract class. 2. (4 marks) We want to implement a simple application that manages the viruses stored in research Labs. You are asked to develop a Java application that uses. an array to store all information regarding the viruses maintained in each research Lab and using the newly created data types: Virus and ResearchLab defined in 1). You should provide a menu with the following options: Please use the partial Java code provided with this assignment which prints the menu. You MUST do this lab in groups of a maximum two students. This Programming Assignment counts for 5 marks. You should upload your java program and screen shot of the test on blackboard on time. Any late submission will be penalized (−0.25/ day . Warning: Plagiarism will be reported to the Academic integrity Office CSC301-Data Structures and Algorithms (Fall 2022)
To implement a virus management system, create Virus and ResearchLab classes in Java, using inheritance and an interface. Use an array to store virus information and provide menu options for managing the viruses.
To implement a virus management system, we can create two Java classes: "Virus" and "ResearchLab". The "Virus" class will represent a virus and will have fields such as the official name, discovery date, and discoverer's name. The "ResearchLab" class will represent a research lab and will contain an array to store information about the viruses stored in that lab. To ensure code reusability and structure, we can create an abstract class called "Organism" that both "Virus" and "ResearchLab" classes will inherit from. This abstract class can define common properties and methods related to organisms.
We can create an interface called "Infectious" that specifies the behavior of infectious organisms. The "Virus" class can implement this interface to indicate that it is an infectious agent. By using these classes, we can develop a Java application that manages viruses stored in research labs. The application can provide a menu with options such as adding a new virus, removing a virus, displaying all viruses in a lab, etc. The menu can be implemented using the partial Java code provided, and the functionality can be implemented using the defined classes and data types.
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why amylose doesn't form a gel when hot water is added? can someone explain
How does the bar graph (or line graph) show the pattern you found?
Answer:
It shows number increasing or descreasing due to circumstances.
Explanation:
have a good day :)
When a population. is declining, what two factors are likely outpacing what
other two factors?
Answer:
Not sure sorry
Explanation:
When do cells duplicate their DNA?
How do cells make accurate copies of DNA?
How do cells make accurate copies of DNA?
Answer:
dont know raadi solve your self
guys help with this plzz
brainlest even if u try ur best :
Vasoconstriction and Vasodilation
__________ carries heat from the core around the body.
As blood flows through the ______________ close to the surface of the ______, heat is lost to the body’s surroundings.
The __________________ which supply the capillaries in the skin have smooth _________ in their walls. This means that the arterioles can dilate or constrict to ____________ the flow of blood into the capillaries.
Explanation:
is it only vasoconstriction and vasodilation as the answers or are there more?
When Mason was a todder, he had to have many blood tests which required him to have mutiple needle pokes of the people that drew his blood as a toddler wore whit ab coats when they drew the blood Later, when he went with his
mother to the pharmacy he was tightened by the pharmacists weanng a whies lab coat. What is this an example of
O cognitive mapping
O observational leaming
O classical conditioning
O operant conditioning
When Mason was a todder, he had to have many blood tests which required him to have multiple needle pokes of the people that drew his blood as a toddler wore whit ab coats when they drew the blood Later, when he went with his mother to the pharmacy he was tightened by the pharmacists weaning a whites lab coat is an example of classical conditioning.
Classical conditioning is a type of learning in which a previously neutral stimulus acquires the ability to produce a response that was originally produced by another stimulus. In this case, the neutral stimulus is the white lab coat. Initially, the lab coat had no special significance to Mason.
However, after being exposed to medical personnel wearing white lab coats during his blood tests, Mason learned to associate the white lab coat with the pain caused by the needle pokes. As a result, when Mason saw the pharmacist wearing a white lab coat, he experienced fear and anxiety.
Classical conditioning is a type of learning that occurs through repeated association of two or more different stimuli. The result of classical conditioning is that the neutral stimulus eventually comes to elicit the response originally produced by the unconditioned stimulus.
The unconditioned stimulus is the stimulus that produces the natural, unlearned response. In this example, the unconditioned stimulus is the pain caused by the needle pokes during the blood tests. The unconditioned response is the pain and discomfort experienced by Mason in response to the needle pokes.
Over time, the previously neutral stimulus (the white lab coat) becomes associated with the unconditioned stimulus (the pain from the needle pokes). As a result, the white lab coat becomes a conditioned stimulus that elicits a conditioned response (fear and anxiety) in Mason. This is an example of classical conditioning.
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help plssss
What unicellular protists have shells?
Answer:
Foraminifera (forams for short) are characterized as unicellular protists that have shells
What molecules are needed for cellular respiration to happen?.
which of the following is not a pre-zygotic isolating mechanism? select one: a. temporal isolation b. habitat isolation c. hybrid inviability d. gametic incompatibility
The option that is not a pre-zygotic isolating mechanism is hybrid inviability.
Pre-zygotic isolating mechanisms are barriers that prevent mating or fertilization between different species. Temporal isolation (a), habitat isolation (b), and gametic incompatibility (d) are all pre-zygotic mechanisms. Hybrid inviability (c) is a post-zygotic isolating mechanism, as it occurs after fertilization and involves the failure of a hybrid offspring to survive or reproduce.
Among the given options, hybrid inviability is not a pre-zygotic isolating mechanism.
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The top of a dog's head is
A) anterior.
B) ventral.
C) caudal.
D) dorsal.
E) posterior.
The top of a dog's head is D) dorsal. The top of a dog's head is best described as "dorsal." The term "dorsal" refers to the upper side or back of an animal.
In anatomical terms, it is the opposite of ventral, which refers to the underside or belly. When referring to the head, the dorsal side corresponds to the upper portion, including the skull and top of the head.
In the case of a dog, its dorsal side can be observed by visualizing the shape and structure of the head, where the skull is located. This is the area that would be touched or petted when someone strokes the dog's head.
The ventral side, on the other hand, would be the underside of the dog's head, including the lower jaw and neck.
Therefore, the correct answer is D) dorsal.
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what’s the answer to this question?
RR is hom--ogenous red
Rr is heter---ogenous red
R is dominant
r is recessive
What is the Puneet square used for?A Punnett square is a graphical representation used in genetics to predict the probability of an offspring having a particular genotype (genetic makeup) based on the genotypes of the parents. The Punnett square is named after Reginald Punnett, the British geneticist who first described it.
The Punnett square is a simple and effective way to visualize the possible outcomes of a genetic cross between two individuals, where the columns represent one parent and the rows represent the other parent. The cells of the Punnett square contain the possible offspring genotypes that result from the combination of alleles (versions of a gene) from each parent.
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