Who is the scientist for whom the dideoxy sequencing method is named?

Answers

Answer 1

Frederick Sanger is the scientist for whom the dideoxy sequencing method is named.

The Sanger technique of DNA sequencing relies on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication and uses electrophoresis. It was created by Frederick Sanger and colleagues in 1977, and for the next 40 years it was the most extensively utilized sequencing technique. Applied Biosystems first made it available for purchase in 1986. More recently, dideoxy sequencing techniques have supplanted higher volume Sanger sequencing, particularly for large-scale, automated genome analysis. However, for smaller-scale initiatives and for validating the outcomes of deep sequencing, the Sanger approach is still widely used. A single-stranded DNA template, a DNA primer, a DNA polymerase, regular deoxynucleotide triphosphates (dNTPs), and modified di-deoxynucleotide triphosphates (ddNTPs), which stop DNA strand elongation, are needed for the traditional chain-termination approach. When a modified ddNTP is added, DNA polymerase stops extending DNA because these chain-terminating nucleotides lack the 3'-OH group necessary to generate a phosphodiester bond between two nucleotides. The ddNTPs can be fluorescently or radioactively labeled to be detected by automated sequencing equipment.

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Related Questions

What other steps could you have taken to measure effects of exercise on the body?

Answers

Answer:

1.check your BMI. 2.get a measuring tape. 3.get a body scale.

Explanation:

Sana maka tulong

Which animal has a bilaterally symmetrical body plan?

A jellyfish.

A lobster.

Coral.

Answers

Lobster is the answer. It can be split evenly down the middle
Lobster is the answer

Both prokaryotes and eukaryotes have small, free-floating organelles. The cell makes these organelles from nucleic acids and amino acids found in food. Without these organelles, the cell could not make proteins. What are these organelles called?

Answers

Answer: ribosomes

Explanation:

Which animal is an inverterbrate?

A.trout
B. cat
C. snake
D. termite​

Answers

Answer:

Termites

Explanation:

A termite is classified as an insect, and insects are inverterbrate.

Termites because termites are invertebrates

like other eukaryotes, arbuscular mycorrhizal fungi, such as rhizophagus irregularis, are classified into phylogenies using particular approaches. classification of prokaryotes often involves analysis of the 16s ribosomal subunit. what approach is used to develop phylogenies for eukaryotes such as r. irregularis?

Answers

The life activities of microbial cells, including growth, are independent. Arbuscular mycorrhizal fungi, including Rhizophagus irregularis, are categorized into phylogenies using specific methods, just like other eukaryotes. Analysis of the 16S ribosomal subunit is frequently used in the classification of prokaryotes.

What groups of prokaryotes are there?

The two prokaryote kingdoms, Bacteria and Archaea, split apart early in the history of life. There are many different types of bacteria, including pathogens that cause disease as well as beneficial photosynthesizers and symbionts

How are prokaryotes and eukaryotes categorized?

The difference between prokaryotes and eukaryotes is based on the traits of their cells. For instance, whereas eukaryotic cells have both a nucleus and other membrane-bound structures known as organelles, prokaryotic cells do not.

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most absorption and secretion occurs in this part of the nephron.

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Most absorption and secretion occurs in the proximal convoluted tubule of the nephron.


1. Filtration: Blood enters the glomerulus within the Bowman's capsule, where waste products, ions, and water are filtered out of the blood.
2. Reabsorption: The filtered fluid then passes through the proximal convoluted tubule, where most absorption and secretion occurs. Essential nutrients, ions, and water are reabsorbed back into the bloodstream.
3. Loop of Henle: The fluid continues through the loop of Henle, where further adjustments to the concentration of ions and water are made.
4. Distal convoluted tubule: More fine-tuning of the fluid's composition takes place in the distal convoluted tubule.
5. Collecting duct: Finally, the fluid, now transformed into urine, passes through the collecting duct and is excreted from the body.

In summary, most absorption and secretion in the nephron occur in the proximal convoluted tubule.

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Fill in the blanks. The principle behind stacking buckshot pellets is that the____the angle of surface contact between two pellets, the ____the tendency those pellets will impart energy in that direction.

Answers

The principle behind stacking buckshot pellets is that the greater the angle of surface contact between two pellets, the stronger the tendency those pellets will impart energy in that direction.

What is pellets?

Pellets are small, compressed or condensed masses of material, typically made from a variety of substances including wood, metal, plastic, and chemicals. They can be used for a range of applications, such as fuel, ammunition, or as feed for animals.

What is surface contact?

Surface contact refers to the physical contact between two objects or surfaces that are in close proximity to each other. It is the area where the surfaces touch each other and can have an effect on friction and other physical properties.

According to the given information:

The principle behind stacking buckshot pellets is that the smaller the angle of surface contact between two pellets, the greater the tendency those pellets will impart energy in that direction. This is because when the pellets are stacked closely together, they create a more solid and compact mass which allows for more force to be concentrated in a specific direction upon impact. This technique is often used in shotguns for hunting and self-defense purposes.
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Drag each tile to the correct location. classify the events according to the gas they primarily contribute to the atmosphere. cattle digest food. animals perform respiration. forest fires burn. plants perform photosynthesis.

Answers

Cattle digest food - methane and carbon dioxide

Animals perform respiration - carbon dioxide

Forest fires burn - carbon dioxide, carbon monoxide, and methane

Plants perform photosynthesis = oxygen

Events and the gas they contribute

Cattles are able to digest cellulose, fibers, and their likes through the activities of certain bacteria. The process happens through the enteric fermentation of these materials. Thus, carbon dioxide is produced as well as methane.

During respiration, carbohydrates in the form of glucose are broken down to extract the lock-up energy for biological processes. In addition to water, carbon dioxide is also produced as a by-product.

Burning forests leads to the emission of gases like methane, carbon dioxide, and carbon monoxide.

During photosynthesis, inorganic materials are used to synthesize carbohydrates with the by-product being oxygen gas.

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Events according to the gas they primarily contributed to the atmosphere:

Oxygen - Plants perform photosynthesis

Methane - Cattle digest food

Carbon dioxide - Forest fires burn / Animals perform respiration

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which of the following best describes what we mean by gravitational lensing? which of the following best describes what we mean by gravitational lensing? a massive object scatters away the light of more distant objects. telescope lenses are distorted by gravity. a massive object bends light beams that are passing nearby. gravity causes matter that would otherwise be dark to become visi

Answers

The best description of gravitational lensing among the options you provided is: "A massive object bends light beams that are passing nearby."

Gravitational lensing is a phenomenon in which the path of light from a distant object is curved by the gravitational field of a massive object (such as a galaxy or a black hole) that lies between the source of light and the observer.

This bending of light creates a distortion or magnification of the image of the distant object, similar to how a lens in a telescope can bend and focus light to form an image. The effect is caused by the gravitational force acting as a "lens" in space, hence the term "gravitational lensing."

Gravitational lensing provides a unique way to study the properties of massive objects and the structure of the universe, allowing astronomers to probe distant and otherwise inaccessible regions of space.

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vasopressin is a hormone produced in the hypothalamus that regulates the concentration gradients of body fluids. vasopressin is 5.94% s by mass, and each vasopressin molecule contains 2 sulfur atoms. calculate the molar mass of vasopressin

Answers

The molar mass of vasopressin is 1082 g/mol.

To calculate the molar mass of vasopressin, we need to know the atomic masses of the elements that make up the molecule.

First, let's calculate the mass of sulfur in vasopressin. Since each molecule contains 2 sulfur atoms, the mass of sulfur in vasopressin can be calculated as follows:

mass of sulfur = 2 * mass of 1 sulfur atom = 2 * 32 g/mol = 64 g/mol

Next, let's find the total mass of vasopressin. According to the information given, vasopressin is 5.94% sulfur by mass, so the total mass can be calculated as follows:

mass of vasopressin = (mass of sulfur) / (percent sulfur by mass) = (64 g/mol) / (5.94%) = 1082 g/mol

Therefore, the molar mass of vasopressin is 1082 g/mol.

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bariatric surgery is a medical weight loss intervention. it is used to treat people with extreme obesity (bmi greater than or equal to 40) who have not been able to lose weight with other weight management strategies. complete each sentence with the appropriate term.

Answers

Bariatric surgery is a type of surgical intervention used to treat obesity. Bariatric surgery is an option for people with a body mass index (BMI) of 40 or greater who have not been able to lose weight through other methods.

Bariatric surgery works by reducing the size of the stomach or bypassing part of the small intestine, leading to a reduction in food intake and calorie absorption.

Bariatric surgery can lead to significant weight loss, with some patients losing as much as 50% of their excess body weight.

Bariatric surgery is not without risks and potential complications, including infection, bleeding, and nutritional deficiencies.

Long-term success with bariatric surgery depends on making lifestyle changes, including eating a healthy diet and getting regular exercise.

Bariatric surgery is typically covered by insurance when it is deemed medically necessary for the treatment of severe obesity.

Bariatric surgery is not a quick fix for obesity and requires significant commitment to lifestyle changes in order to achieve and maintain weight loss.  

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Given the DNA sequence TAC, what is the tRNA anticodon?

Answers

Answer:

ATG

Explanation:

T (thymine) pairs with the base A (adenine) and C (cytosine) pairs with the base G (guanine)

Answer:

The correct is UAC.

Explanation:

I will be back to explain. I do not remember at the moment. Also I took a test and the person that said ATG is wrong.

What is artificial selection??
A.) a natural process

B.) A process during which human intentionally breed organisms with desirable traits.

C.) A process during which humans speed up the process of reproduction in organisms.

D.) A process during which humans genetically modify an organisms DNA.​

Answers

artificial selection is a process during which humans intentionally breed organisms with desirable traits. The answer is B.

Answer:

i believe the answer is b

Explanation:

Match the agency with the type of event over which it takes jurisdiction.

1. Bureau of Land Management
2. Food and Drug Administration
3. United States Department of Agriculture

A wildfire is destroying national forests in California.
A farmer in Kansas wants to obtain energy from a wind farm on federal land.
An outbreak of mad cow disease is reported.

Answers

Answer:

A wildfire is destroying national forests in California.-3

A farmer in Kansas wants to obtain energy from a wind farm on federal land.-1

An outbreak of mad cow disease is reported.-2

Explanation:

Hope this helps <3

~Grey

Answer:

3 A wildfire destroying national forests in California

2 an outbreak of mad cow disease is reported

1 a farmer in Kansas want to obtain energy from a wind farm on federal land

Explanation: I got it right on a quiz that I did in the 11th grade your welcome and plz give me the Brainiest tanks

which structure is unique to living organisms​

Answers

Answer:

i would say the cell itself is unique

Explanation:

Answer:

cell because it is the smallest unit of our life and it is the building block of living organisms

How does a geneticist see the world differently than the common man?

Answers

Geneticists are the scientists and they see the world differently than that of the common man because they apply scientific approach to life.

Who are Geneticists?

A geneticist is a biologist or the physician who studies genetics branch of science, the science of genes, heredity, and variation of organisms through time. A geneticist can be employed as a scientist or as a lecturer.

A scientist who has the special training in study of genes and heredity including the passing of genetic information from parents to their children. A medical geneticist is a doctor who specializes in the diagnosing and treating genetic disorders or conditions.

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Explain how humans have changed Earth over the last century.

Answers

Answer:

On Earth, human activities are changing the natural greenhouse. Over the last century the burning of fossil fuels like coal and oil has increased the concentration of atmospheric carbon dioxide (CO 2). This happens because the coal or oil burning process combines carbon with oxygen in the air to make CO 2. To a lesser extent, the clearing of land for agriculture, industry, and other human activities has increased concentrations of greenhouse gases.

The glycemic index of carrots is quite high. Why is the glycemic index sometimes misleading? a) It's relative to serving size b) It includes digestible fiber c) It only counts for sugars d) It's relative to the amount of carbohydrate

Answers

The glycemic index (GI) measures how quickly foods containing carbohydrates raise blood sugar levels. One reason is that the GI is relative to the serving size of the food being consumed. The correct option is (a).

Eating a larger portion of a food with a high GI will result in a larger increase in blood sugar levels, whereas a smaller portion of the same food will have a smaller effect.One reason is that the GI is relative to the serving size of the food being consumed. Eating a larger portion of a food with a high GI will result in a larger increase in blood sugar levels, whereas a smaller portion of the same food will have a smaller effect.

Another reason is that the GI does not take into account other components of food that can affect blood sugar levels, such as fiber. Fiber slows down the digestion and absorption of carbohydrates, resulting in a lower GI.Additionally, the GI only measures the impact of sugars and starches on blood sugar levels, but other components of foods, such as fat and protein, can also affect blood sugar levels.

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To best protect the biodiversity of the world when purchasing a pet you should. A) purchase only captive bred species. 56) One challenge to the ...

Answers

To best protect the biodiversity of the world when purchasing a pet is that you should A) purchase only captive bred species.

Purchasing captive bred species helps to ensure that wild populations are not depleted and that the natural balance of ecosystems is maintained.

This approach also discourages illegal wildlife trade and reduces the risk of introducing non-native species into local environments.


In summary, to protect global biodiversity when buying a pet, it is important to choose captive bred species, as this supports the conservation of wild populations and ecosystems.

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What best describes the transport process occuring in the model?

What best describes the transport process occuring in the model?

Answers

Facilitated diffusion

Color Blindness is a recessive, sex-linked trait in humans.A boy is colorblind. His father is colorblind. His mother is not. Which parent gave the boy the colorblind gene?

Answers

The mother is the parent that gave the boy the colorblind gene.

What is color blindness?

In humans, color blindness is a recessive, sex-linked trait that is carried on the X chromosome.

Since males only inherit one X chromosome from their mother and one Y chromosome from their father, they are more likely to express sex-linked recessive traits if they inherit the affected allele from their mother.

In this scenario, the boy's father is colorblind, which means he must have inherited the colorblind gene from his mother since his father could not have passed it on to him (his father could only pass on the Y chromosome to him).

The mother is not colorblind, which means she must have inherited at least one normal allele for color vision. Since the boy is colorblind, he must have inherited the colorblind allele from his mother, who must be a carrier for the trait.

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Having hair on the back of the hand is a dominant trait, and not having the
hair is a recessive trait. Which set of parents will have only children without
hair on the backs of their hands?
O A. A mother with hh and a father with Hh
B. A mother with hh and a father with hh
O C. A mother with Hh and a father with Hh
O D. A mother with HH and a father with Hh

Answers

Answer:

B

Explanation:

This is because these parents solely have no hair on the back of their hands, and don't have the trait to possibly give their child hair on the child's hands.

In a DNA molecule, the base pairs provide genetic information, and the sugar-phosphate backbone does not, because _______________________ .

Answers

In a DNA molecule, the base pairs provide genetic information, and the sugar-phosphate backbone does not, because the genetic information is encoded in the sequence of the nitrogenous bases, not in the sugar-phosphate backbone.

The sugar-phosphate backbone of DNA is composed of alternating sugar (deoxyribose) and phosphate molecules. It forms the structural framework of the DNA molecule and provides stability to the double helix structure. The phosphate groups within the backbone create a negative charge, helping to repel each other and maintain the helical shape.

The nitrogenous bases, on the other hand, are attached to the sugar molecules and project inward toward the center of the DNA molecule. The four nitrogenous bases in DNA are adenine (A), thymine (T), cytosine (C), and guanine (G).

The specific sequence of these bases along the DNA molecule is what carries the genetic information. The base pairing rules dictate that A pairs with T, and C pairs with G, forming complementary base pairs that stabilize the double helix structure.

Thus, it is the specific arrangement and sequence of the base pairs that contain the genetic information, determining the genetic code and the instructions for protein synthesis, while the sugar-phosphate backbone provides structural support but does not carry the same level of genetic information.

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1. On average, how do the proportions of typical and mutant bacteria change in the population over time? ​

Answers

Answer:

bacteria

Explanation:

Organisms that show similar patterns of growth while they are in the embryonic stage have what type of homology?

Answers

Answer:

Developmental Homology.

Explanation:

Answer:

Development Homology

Explanation:

Comparing the embryos of different animals can show relationships that can't be seen when comparing the fully formed animals.Evidence of an evolutionary common ancestor is seen in the similarity of embryos in markedly different species. Darwin used the science of embryology to support his conclusions. Embryos and the development of embryos of various species within a class are similar even if their adult forms look nothing alike.

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5. Membrane receptors are specialized proteins that take part in communication
between the cell and the outside world. The membrane protein receptor can be
subdivided into three parts or domains. The portion of the protein in the E zone is
extracellular (outside of the cell). The P zone is the plasma membrane and the
zone is intracellular (inside of the cell)
P
Moteur
A glucagon hormone is a molecule that stimulates a reaction inside liver cells.
Based on the diagram, where do you think a glucagon hormone most likely binds
to send its signal to the cell?

5. Membrane receptors are specialized proteins that take part in communicationbetween the cell and the

Answers

Answer:

The E zone portion of the protein

Explanation:

The glucagon hormone which is a signal molecules that triggers a reaction in the liver cells is most likely to bind to the E zone extracellular portion of the G-protein-coupled receptor. This then changes the conformation of the receptor activating the heteromeric G proteins.

Answer:

The E zone portion of the protein

Explanation:

Explain how the processes of cellular respiration, photosynthesis, and carbon cycle are related to each other.

Answers

Cellular respiration, photosynthesis, and the carbon cycle are all interrelated and interdependent biological processes. Cellular respiration converts organic compounds into ATP that can be used to fuel metabolic activities, whereas photosynthesis is the process by which green plants and algae convert light energy into chemical energy, in the form of organic compounds.

Cellular respiration produces carbon dioxide and water, which are released into the atmosphere. In contrast, photosynthesis absorbs carbon dioxide and water, as well as sunlight, and uses them to create glucose and other organic compounds.

Thus, cellular respiration and photosynthesis are linked because the products of one process are the reactants of the other.The carbon cycle, on the other hand, involves the transfer of carbon between living organisms, the atmosphere, and the geosphere. In this process, carbon is taken up by green plants and other photosynthetic organisms, where it is used to synthesize organic compounds.

This carbon is then released back into the atmosphere through cellular respiration or is passed on to other organisms. Some carbon is also buried in the ground as fossil fuels. Thus, the carbon cycle is closely tied to both cellular respiration and photosynthesis.

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What are the pros and cons of Gene Therapy?

Answers

Pros of gene therapy -
1.Gene therapy is a source of hope
2.The effects of therapy are long lasting and timeless
3. Gene therapy can eradicate disease and improve the quality of life

The cons of gene therapy
1. There is no guarantee and it can go wrong
2. Gene therapy is a costly treatment option
3. There is a potential for developing resistance
4. Ethical concerns

Two plants cross-fertilize. One plant has round yellow seeds with the genotype RRYY; the other parent plant has green and wrinkled seeds rryy. What gametes can each parent plant produce

Answers

Each parent plant can produce two types of gametes. The first plant, with round yellow seeds, can produce gametes with the genotype RY and Ry. The second plant, with wrinkled green seeds, can produce gametes with the genotype rY and ry.

Through cross-fertilization, these gametes can combine to form offspring with the hybrid genotype RrYy. This process enables the two parent plants to share their genetic material in order to create a new, diverse population of plants.

During cross-fertilization, the two parents exchange genetic information when their gametes combine. The two parent plants each have their own set of alleles, which are the alternative forms of a gene. The alleles from each parent plant can be combined, producing a unique combination in the offspring.

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please helpppppppppppppp

please helpppppppppppppp

Answers

Answer:

alike- plants absorb the light energy they covert it into chemical energy for their own nutrition.

different- energy stored in the bonds of atoms and molecules

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