proteins are directly transferred from the gel to a membrane sheet of charged nylon call nitrocellulose

Answers

Answer 1

Nucleic acids are transported from gels to membranes for further analytical research employing blotting techniques after gel electrophoresis. Southern blotting is used to transfer DNA from agarose gels to membranes and analyze the composition of the gels.

The transfer buffer is drawn through the agarose gel by capillary action when the hybridization membrane is placed between the gel and any absorbent material. For this, the agarose gel is put on a wick made of filter paper. The buffer flow draws the DNA molecules, which are then fixed on the artificial membrane. Northern blotting is used for RNA analysis; the absorbent material differs between the two techniques. Western blotting is the process used to move proteins from acrylamide gels to membranes. In order to create a dot on a membrane, a mixture containing the chemical responsible for that appearance must be applied directly to the membrane or paper substrate. This differs from standard blotting methods in that the materials are not gel electrophoresis. B is the proper response. Protein electrophoresis bands from a polyacrylamide gel were used in the experiment.

The complete question is:

__________ proteins are directly transferred from the gel to a membrane sheet of charged nylon called nitrocellulose.

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Related Questions

Calculate the population density of 900 sheep in a plot of land that is 3.00 km by 2.00km

Answers

The population density of the 900 sheep in the given plot of land is 150 sheep per square kilometer.

The population density of 900 sheep in a plot of land that is 3.00 km by 2.00 km can be calculated by dividing the total number of sheep by the area of the land.

First, we need to calculate the area of the land. The area can be found by multiplying the length and width of the plot of land:

Area = length × width = 3.00 km × 2.00 km = 6.00 km²

Next, we divide the total number of sheep by the area to calculate the population density:

Population Density = Total number of sheep / Area = 900 sheep / 6.00 km²

Performing the calculation, we find:

Population Density = 150 sheep/km²

Therefore, the population density of the 900 sheep in the given plot of land is 150 sheep per square kilometer.

Population density is a measure of the number of individuals (in this case, sheep) per unit area. By dividing the total number of sheep by the area of the land, we obtain the population density in terms of sheep per square kilometer. In this case, the population density is 150 sheep/km², indicating that there are, on average, 150 sheep within each square kilometer of the land.

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Amir is asked to read this data chart on the Earth’s layers and temperature. Which layer represents the crust?

Data table containing the following four column headings. Layer number, Temperature Range in Celsius, Average Density in Gram per cubic centimeter, and Composition. Four rows are seen with the following readings. Layer 1, 4400-6100, 11.1, liquid nickel and iron. Layer 2, 200-400, 3.0, solid rock. Layer 3, 5000-7000, 12.8, solid nickel and iron. Layer 4, 500-4000, 4.5, molten rock.

A.
Layer 1

B.
Layer 2

C.
Layer 3

D.
Layer 4

Answers

Answer:

b.layer2

Explanation:“Crust” describes the outermost shell of a terrestrial planet. Our planet's thin, 40-kilometer (25-mile) deep crust—just 1% of Earth's mass—contains all known life in the universe. Earth has three layers: the crust, the mantle, and the core. The crust is made of solid rocks and minerals.

hope its help

What molecules are created by the organism during photosynthesis? Where will these products go in your system?

Answers

Answer:

carbohydrate molecules

Read each of the sentences that describe a phase of meiosis. Place each sentence into the correct box.

Homologous chromosomes pair to form a tetrad.

Crossing-over may occur between the non-sister chromatids.

Homologous chromosomes line up at the center of the cell.

Homologous pairs separate and move towards opposite ends of the cell.

A nuclear membrane forms and cytokinesis follows.

The haploid number of duplicated chromosomes line up at the metaphase plate.

Sister chromatids separate and become daughter chromosomes that move to the poles.

Four haploid daughter cells are formed that are not genetically identical.

Prophase 1

Metaphase

Anaphase and Telophase !

Meiosis 11

Answers

Answer:

prophase 1(Homologous chromosomes pair to form a tetrad.

prophase 1(Crossing-over may occur between the non-sister chromatids).

Metaphase 1 (Homologous chromosomes line up at the center of the cell.)

Anaphase 1 (Homologous pairs separate and move towards opposite ends of the cell)

Telophase 1 (A nuclear membrane forms and cytokinesis follows).

Metaphase 2 ( The haploid number of duplicated chromosomes line up at the metaphase plate.

Anaphase 2 (Sister chromatids separate and become daughter chromosomes that move to the poles)

Meiosis 2: Four haploid daughter cells are formed that are not genetically identical.

Explanation:

Meiosis is a type of cell division that gives rise to gametes ( offsprings). This type of cell division occurs only in the reproductive organs. A diploid cell has two sets of chromosomes: one from the female parent and the other one from the male parent. When the diploid cell undergoes meiosis, the chromosome replicate once and the nucleus and cell duplicate twice giving rise to four haploid gamete cells.

The phases of meiosis occurs in two step divisions. Homologous pairs separate during the first round cell division which occurs in MEIOSIS 1 while the sister chromatids separate in the second nuclear division called MEIOSIS 2.

In each round of division, cells go through four stages: PROPHASE, METAPHASE, ANAPHASE, and TELOPHASE. The specific events that occur in these stages are already listed in the above answer.

Generally, at the start of meiosis, each member of a homologous pair of chromosomes, which are made up of two chromatids, moves to lie side by side. While they are thus paired, genetic material is exchanged between the chromatids. This is known as CROSSING OVER.

When the nucleus divides for the first time, the chromosomes in a given pair( not the chromatids) separate and move to opposite ends of the cell. This results in only half the number of chromosomes going to each daughter cell.

During the second nuclear division, the chromatids separate and move to opposite ends of each daughter cell giving rise to four gamete cells, each with a haploid number of chromosomes.

Meiosis is the process through which haploid gametes are formed from a diploid germ cell. It takes place in two phases of four steps each: prophase, metaphase, anaphase and telophase.  

---------------------------------

Through Meiosis, a diploid cell (2n) produces four haploid daughter cells (n).  

After DNA replication there are two meiotic phases.  

The first one is a reductive phase, in which homologous chromosomes separate.  

In the second phase, the cell suffers a new, not reductive division.  

1. In the first phase, Meiosis I:  

Prophase I: Chromosomes condensate and become visible. Occurs crossing-over between homologous chromosomes. Crossin-over makes the daughter cells to be genetically different from the original one.

Metaphase I: Homologous chromosomes randomly align in the equatorial plane.  

Anaphase I: In this phase occurs the division and independent separation of homologous pairs. Each chromosome migrates to different poles. This separation generates different chromosomal combinations in the daughter cells.  

Telophase I: Chromosomes of homologous pairs are already in the corresponding poles, and the nuclear membrane forms again in each pole.  

2. In the second phase, Meiosis II:  

Prophase II: Chromosomes condensate again and become visible.  

Metaphase II: Chromosomes join the spindle apparatus and migrate to the equatorial plane, where they randomly line up. Sister chromatids are holden together until they reach the Anaphase.  

Anaphase II: Centromeres divide, chromatids get separated, and each of them goes forward an opposite cellular pole.  

Telophase II: Once in the poles, the chromosomes become lax again, and the nuclear membrane forms again.  

Cytokinesis occurs at the end of Meiosis II. The final products are four haploid cells different from the original one.  

According to this information, the correct order would be as follows

Prophase 1:  Homologous chromosomes pair to form a tetrad.

Prophase 1:  Crossing-over may occur between the non-sister chromatids.

Metaphase 1: Homologous chromosomes line up at the center of the cell.

Anaphase 1: Homologous pairs separate and move towards opposite ends of the cell.

Telophase 1: A nuclear membrane forms and cytokinesis follows.

Metaphase 2: The haploid number of duplicated chromosomes line up at the metaphase plate.

Anaphase 2: Sister chromatids separate and become daughter chromosomes that move to the poles.

Meiosis 2: Four haploid daughter cells are formed that are not genetically identical.

---------------------

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What is a population bottleneck?

Answers

Answer:

A population bottleneck or genetic bottleneck is a sharp reduction in the size of a population due to environmental events such as famines, earthquakes, floods, fires, disease, and droughts or human activities such as specicide and human population planning.

Explanation:

Explanation:

A population bottleneck or genetic bottleneck is a sharp reduction in the size of a population due to environmental events such as famines, earthquakes, floods, fires, disease, and droughts or human activities such as specicide and human population planning.follow me

ormed by waves eroding less - resistant rocks along the shore

Answers

Costal Erosion is the form of erosion that is caused when waves of seas hit the rocks on the shore.

What is Erosion ?

The removal of soil, rock, or dissolved material from one area on the Earth's crust and subsequent transport to another region for deposition are known as erosional processes. Erosion differs from weathering, which is a static process.

Earthen materials are worn away during erosion, a geological process in which they are moved by wind or water. Weathering, a related process that does not involve movement, dissolves or breaks down rock.

When materials like boulders and stones carried by waves strike and knock against one other, they are said to be attritionally worn away. Sand and rounded beach pebbles are created via the wear and tear of these materials. One of the most obvious signs of coastal erosion is the formation of sea cliffs.

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specialied cells work together to form____ that make up_____

Answers

Answer:

specialied cells work together to form a tissue that make up all structure and content of your body

Explanation:

correct me if I'm wrong

Sana makatulong

By the time a cell has reached metaphase, all the following have occurred except...

Answers

Answer:

C. Sister chromatids separate

Explanation:

During interphase, which is composed of the G1 phase, S phase and G2 phase, the cell grows and replicates its DNA and some of its organelles. During the S phase, the genetic material is duplicated into two sister chromatids, and centrosomes, which are nucleated microtubules that interact with the chromosomes to form the mitotic spindle, are also replicated. Metaphase is the third phase of mitosis, where sister chromatids align in the middle of the cell before their separation into two daughter cells.

Which statement summarizes the relationship between chromosomes,DNA, and genes

Answers

Answer:

The accurate answer to this is:

D.Genes use DNA code to manufacture proteins for chromosomes.

Explanation:

Genes use DNA code to manufacture proteins for chromosomes.

What are chromosomes?

The thread-like chromosomes that convey the genomic information from cell to cell are formed of protein and a single DNA molecule.

Chromosomes are found in the nucleus of cells in both plants and animals, including humans. There are 46 chromosomes in all, including 22 pairs of numbered chromosomes (autosomes) and one pair of sex chromosomes (XX or XY).

Children receive half of their chromosomes from their mother and half from their father because each pair comprises two chromosomes, one from each parent.

Therefore, Genes use DNA code to manufacture proteins for chromosomes.

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Shoring can only be used with:
A. Stable rock.
B. Cohesive soil.
C. Fine, sandy soil.
D. Non-cohesive soil.

Answers

Answer: B (Cohesive soil)

Explanation:

Shoring is a method used to support the sides of an excavation or trench, typically used to prevent soil from collapsing and protect workers from falling. Shoring is typically used in cohesive soils, which are soils that have good internal strength and can hold together well. Cohesive soils include clay, silt, and clay loam.

Stable rock can be excavated without the need of shoring, A and D options are not suitable for shoring. In fine, sandy soil (C option) it's not likely to hold together well and the structure of shoring may not be effective.

which bio molecules are indicative of atypical kidney function?

Answers

The bio molecules that serves as the indicative of a typical kidney function are Albuminuria and Proteinuria.

What is are kidney function?

Kidneys  can be described as the  very important organ in the body that remove wastes and extra fluid from your body.

It should be noted that kidneys  help to do away with acid that is produced by the cells  hpowever when there is Proteinuria in the urine it implies that the there is kidney damage. More over the kidney failure can as well be seen when there is Albuminuria in excess in the urine which is not good for the body health.

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A dialyzing machine has a membrane that is permeable to water, urea, uric acid, ammonia, salts, and
glucose. The membrane is impermeable to protein molecules. In relation to the blood flowing
through the dialysis machine, the dialyzing solution must have:

a. the same concentrations of urea and salts

b. lower concentrations of glucose and protein

c. lower concentrations of urea, uric acid, and ammonia and higher or equal concentrations of glucose

d. higher concentrations of urea, uric acid, and ammonia and lower or equal concentrations of glucose

Answers

Answer:

C. lower concentrations of urea, uric acid, and ammonia and higher or equal concentrations of glucose

Explanation:

Hope it helps!!!

The concentration of salts in blood reamins relatively steady regardless of a person's diet. This situation best illustrates


homeostasis


autotrophy


evolution


life's organizational hierarchy

Answers

homeostasis

homeostasis is constant

What concept does the author develop in paragraphs 14-16 of the article?
The tomatoes containing the weed gene are more flavorful than traditional
tomatoes, but they will be rejected by consumers because of their appearance.
B
Because of the controversy surrounding genetically engineered tomatoes, the public
will not have the opportunity to taste them.
Despite their increased sugar levels, the genetically engineered tomatoes actually
lack improved flavor.
The vegetable seed industry is lobbying to persuade the Department of Agriculture
to permit people to eat experimental produce.

Answers

Because of the controversy surrounding genetically engineered

tomatoes, the public will not have the opportunity to taste them

What does the paragraph develop?

We can see when we look at the article that has been shown in the PDF document that has been attached to the question, there is the discussion around the use of the genetically modified food.

This is food that was produced by some sort of genetic recombination. The idea is whether this kind of food could be seen to be safe or not. In effect, people have not been allowed to eat the genetically modified food for fear of safety and this is developed in paragraphs 14-16 of the article.

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What two words best describe bacterial reproduction? Why?

Answers

Answer: Bacteria reproduce through a process called binary fission. During binary fission, the chromosome copies itself, forming two genetically identical copies. Then, the cell enlarges and divides into two new daughter cells. The two daughter cells are identical to the parent cell.

Explanation:

which characteristic is the same for all electromagnetic waves

Answers

Answer:

its frequency

Explanation:

their frequencies remain unchanged but their wavelength changes when the wave travels from one medium to another.

uses of biotechnology in washing powder making

Answers

Answer:

Here are some uses of biotechnology in the production of washing powders:

Enzymes: Enzymes are commonly used in washing powders to enhance the cleaning efficiency and remove various types of stains.  Proteases, amylases, lipases, and cellulases are examples of enzymes used in washing powders. These enzymes break down protein-based, starch-based, lipid-based, and cellulose-based stains, respectively, into smaller fragments, making them easier to remove during the washing process.

Bio-surfactants: Surfactants are essential components of washing powders as they help to remove dirt and oil from fabrics. Biotechnology allows for the development of bio-surfactants, which are surfactants derived from biological sources, such as bacteria or yeast. These bio-surfactants are more environmentally friendly than traditional surfactants as they are biodegradable and have lower ecological impact.

Microbial Strains: Biotechnology can be used to isolate, characterize, and optimize microbial strains that produce enzymes or bio-surfactants. Microorganisms, such as bacteria and fungi, can be engineered or selected for their ability to produce specific compounds that enhance the cleaning performance of washing powders.

Genetic Modification: Genetic modification techniques can be employed to enhance the properties of microorganisms used in the production of enzymes or bio-surfactants. This allows for the creation of microorganisms with improved characteristics, such as increased enzyme activity or enhanced stability under varying conditions.

Fermentation Processes: Biotechnology facilitates the use of fermentation processes to produce enzymes and bio-surfactants at a large scale. Fermentation involves the controlled growth of microorganisms in bioreactors, where they produce enzymes or bio-surfactants through metabolic processes. This enables the efficient and cost-effective production of these ingredients for use in washing powders.

Explanation:

hello help me with this question thanks in advance

don't spam​

hello help me with this question thanks in advancedon't spam

Answers

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Renewable resources are those resources which are inexhaustible in nature . It means these resourses are present in unlimited quantity and are environment friendly.

Geothermal energy is one of the example of renewable resources.

In geothermal energy, The word ' geo' stands for earth. So ,It basically means the energy that is extracted from the earth.

Here are some uses for the utilisation of renewable geothermal energy.

Geothermal energy helps in generating electricity.

The geothermal power plant extract the heat from geothermal reservoirs. Then, This extracted heat is transfer to the heat engine .Which are responsible for running the heat engine smoothly and generation of electricity.

Geothermal energy helps in heating greenhouse .

The heat that is released from the underground reservoirs are used to reduce green house gases because Geothermal energy needs very less CO2 . This heat is transfer in green house and it results into direct heating of green houses.

It also act as a tourist spot

There are so many people who wish to enjoy their leisure time peacefully. In winters, People like to visit the areas where hot spots are found .Which is a example of geothermal energy.

Geothermal energy helps in heating and cooling buildings

The warm water is taken from underground reservoirs and then converted into hear energy. Then this heat energy is transfer in the building by using geothemal pumps helps in generating heat in building in winter. This same process is also follows for cooling of building.

It is also used as industrial purpose

Geothermal energy is also used in industries for cooking, pastuerizing milk etc.

the diploid number of chromosomes in the cell of a domesticated dog is 78. which of the following options include.___after fertilziaton.

Answers

39 chromosomes involve at the cell poles as a result. They decreases from 2n (diploid) to n (haploid). They then go through meiosis II, results in 4 haploid daughter cells, with half several chromosomes as the parent cell.

What is meiosis?

A single cell has been splits twice during the meiotic process, leaving four cells with half of its original genetic material. These cells sperm in men and eggs in women are our sex cells.

When DNA is broken, the G2 checkpoint prevents cells from initiating mitosis, giving the chance for healing and limiting the reproduction of damaged cells. Understanding the molecular origins of cancer requires a focus on the G2 checkpoint since it helps to the creation of genomic stability.

Therefore, They then go through meiosis II, results in 4 haploid daughter cells, with half several chromosomes as the parent cell.

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You are studying an alien bacteria-like organism isolated from the clouds of Venus. You decide to repeat the Meselson-Stahl experiment to find out how it replicates its DNA. This time you plan to grow the cells on light 14N medium for many generations first. Then, you plan to transfer them to heavy 15N medium and allow them to grow for 2 additional generations (2 rounds of DNA replication). If the alien replicates its DNA via the conservative model of DNA replication, what is the expected distribution of DNA in the density gradient after two rounds of replication

Answers

Answer:

it is expected to observe two bands: one band of light density and one band of heavy density

Explanation:

DNA replication is semiconservative because when DNA is replicated both resulting DNA molecules are composed of one original strand and one newly synthesized strand. If DNA replication would be conservative, then the original DNA molecule would serve as a template to produce a new DNA molecule, thereby each cell division would produce one daughter cell with a new DNA molecule and one daughter cell with the original DNA molecule of the parental cell. In this case, the transfer of bacteria-like organisms into a heavy 15N medium would produce two types of DNA cells after replication: cells with original DNA of light density (14N) and cells with new DNA of heavy density (15N), which would be distributed in two bands after centrifugation.

An organism makes another organism like
itself.
An organism goes through changes and stages
- to become an adult.
A long molecule contains the genetic
- instructions for the organization and metabolism
of an organism.
Changes in the genetic information are
- inheritable and are an important source of
variation in the diversity of life.

Answers

Answer:

I do not understand.Please explain

How do the layers of the atmosphere help to understand better the dynamics of the atmosphere and its relationship to the hydrosphere and lithosphere?

Answers

There are various layers that make up the atmosphere, and each has distinct properties and functions. Both weather generation and energy transmission take place in the troposphere, which is closest to the Earth's surface.

The stratosphere shields the Earth from damaging UV radiation along with the ozone layer. Most auroras happen in the mesosphere, where meteors burn up. Modern technology is dependent on the thermosphere, where the majority of the Earth's satellites orbit and where communication signals are sent.

The dynamics of the atmosphere and its interactions with the hydrosphere and lithosphere are better understood thanks to these layers. Foreseeing and minimising environmental problems like climate change and preserving our world, it is essential to understand the significance of each layer.

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The graph below shows a normal distribution for adult body size in a population of a particular species of fish.

Suppose commercial fishers begin using nets that selectively catch larger fish of this species. They continue using these kinds of nets for many years.

(a) Describe how the size distribution of adult fish in the population would most likely change over time.

(b) Explain, in detail, how natural selection would produce the change you described in part.

(c) Even if the use of nets that selectively catch larger fish were stopped, some biologists believe that the size distribution in the fish population would not return to normal. Explain why.

The graph below shows a normal distribution for adult body size in a population of a particular species

Answers

As the net used by fishermen selectively catches the fish which have a larger body size, the peak of the graph will shift towards the left as the population of small body-sized fish will increase.

The water body contains adult fishes in small as well as large body size and the fishermen start using nets which selectively catch the fishes with the larger body size and they keep using these nets for many years. This will basically result in the higher population of the small sized fish in the water body and the peak of the group will therefore shift towards the left.

The natural selection will also favor the adult fish with the small body size as they have more chances of survival in the given environment over the large sized adult fishes.

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Some organisms have a large geographic range. For example, the Norway rat lives on every continent except Antarctica.

A population of Norway rats is the number of rats in one particular area. Do you think it’s scientifically possible for a population of Norway rats in North America to carry different genetic mutations than a population of Norway rats in Africa? Do you think it’s possible that they carry some of the same mutations? What types of information can scientists learn by studying the genomes of these two populations of rats?

Answers

Norway rats in North America carry different genetic mutations than Norway rats in Africa because of different environmental conditions.

Do you think the Norway rats in North America carry different genetic mutations than Norway rats in Africa?

Yes, it is scientifically possible for a population of Norway rats in North America to carry different genetic mutations than a population of Norway rats in Africa because the environmental conditions of both regions are very much different from one another. So the mutation occurs in the body of rats in order to make some adaptations according to the environmental conditions which help them to survive.

So we can conclude that Norway rats in North America carry different genetic mutations than Norway rats in Africa because of different environmental conditions.

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What happens to the energy that is stored in cells? Check all that apply.

It is released slowly.
It is released quickly.
It is released in a single reaction.
It is released in a series of reactions.
It is used by cells for repair and growth.

Answers

Answer:

it is realesed slowly I think sorry if its not the answer I haven't been in bio in 2 years

Which substance is a product of glycolysis?

Answers

Answer:

Outcomes of Glycolysis

Glycolysis produces 2 ATP, 2 NADH, and 2 pyruvate molecules: Glycolysis, or the aerobic catabolic breakdown of glucose, produces energy in the form of ATP, NADH, and pyruvate, which itself enters the citric acid cycle to produce more energy.

Explanation:

2 ATP, 2 NADH, and 2 pyruvate molecules:

List some of the biotic and abiotic factors that exist in the eco system. (Picture below)

List some of the biotic and abiotic factors that exist in the eco system. (Picture below)

Answers

EXPLANATION/ANSWER:

Biotic factors: things that are living

• grass

,

• impala

,

• ticks

,

• seed-eating bird

,

• locust

,

• scorpion

,

• hawk

,

• tick bird

,

• snake

,

• baboon

,

• leopard

,

• hyena

Abiotic factors: things that are non-living that affect the living

• soil

,

• sunlight

,

• temperature

,

• water

WILL MARL BRAINLIEST!!!!
The islands bordering the deep-sea trenches
A. result from a series of quiet, continuous basaltic eruptions.
B. are accumulations of sediments on the margins of the trenches.
C.are formed from the activities of coral and other organisms.
D. are explosive volcanoes that emit lavas.

Answers

Answer:

i think its d

Explanation:

Which statement describes earthquakes which team explain one way that minerals form

Answers

Earthquakes are natural phenomena characterized by the sudden release of energy in the Earth's crust, resulting in seismic waves that can cause the ground to shake.

During an earthquake, the rocks along the fault experience immense pressure and deformation.

This intense pressure can cause minerals within the rocks to undergo various processes that contribute to their formation.

One way minerals can form as a result of earthquakes is through metamorphism.

Metamorphism is the process by which existing minerals within rocks undergo changes in response to high temperatures, pressures, and deformation.

During an earthquake, the rocks near the fault zone are subjected to extreme pressure and temperature conditions.

The intense stress causes the minerals to recrystallize, rearrange their atomic structures, and form new minerals.

This process is known as dynamic metamorphism or cataclasis.

The newly formed minerals in fault zones can include varieties of quartz, feldspar, mica, and other minerals commonly found in metamorphic rocks.

The specific minerals that form depend on the composition of the original rocks and the conditions present during the earthquake.

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Using the human species as an example, explain what is meant by a variation of traits.​

Answers

Explanation:

a variation of traits could be a variation of personality traits? everybody is different? what I do know Is that variation can be inherited or environmental which means that you get it from your ancestors through DNA or you get it from the environment around you and your choices eg a tattoo

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