Combination of the template DNA, primers, Taq Polymerase, dNTPs, and buffer creates an environment for the selective amplification of a specific DNA fragment by PCR.
PCR (polymerase chain reaction) is a widely used technique in molecular biology that allows the amplification of a specific DNA fragment. The following are the reagents needed for a successful PCR experiment:
DNA template: The target DNA that needs to be amplified serves as the template for PCR. Primers: Short synthetic oligonucleotides that are complementary to the specific DNA sequence flanking the target region. They are designed to anneal to the template DNA and serve as a starting point for the polymerase chain reaction.
Taq Polymerase: A thermostable DNA polymerase enzyme that can withstand high temperatures and can synthesize a new strand of DNA using the template DNA and the primers. Taq Polymerase is used because it has a high processivity and a low error rate.
Deoxynucleotide triphosphates (dNTPs): These are the building blocks of DNA that are used to synthesize the new strand. They are added to the reaction mixture at a concentration of 200 μM each.
Buffer: A solution that provides optimal conditions for the Taq Polymerase activity. It typically contains Tris-HCl (pH 8.3-8.5), KCl, and MgCl2. The role of each reagent is as follows: DNA template: It provides the specific DNA sequence that needs to be amplified.
Primers: They anneal to the template DNA and provide a starting point for the polymerase to synthesize a new strand of DNA. Taq Polymerase: It catalyzes the addition of nucleotides to the growing DNA strand.
dNTPs: They are incorporated into the growing DNA strand to create a new complementary strand. Buffer: It maintains the optimal pH and salt concentration for Taq Polymerase activity and ensures efficient amplification.
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I need help with chemistry question?
Answer:
2Al + 3Na2SO4 -----> Al2(SO4)3 + 6 Na
Explanation:
Product should be Al2(SO4)3
Because Al contains +3 charge
And SO4 contains -2 charge
To balance this postive and negative charge we multiple +3 with 2 and -2 with 3
NEED HELP ASAP PLEASE
Identify the type of bonding, covalent, ionic, or metallic, between
the atoms in an oxygen molecule.
Answer:
here hope it helps
Explanation:
covalent bonds
If pressure is kept constant and the temperature of a gas is doubled, what will happen
to the volume?
A. It will double.
B. It will remain constant
C. It will decrease to 25% of its original volume.
D. It will decrease by half.
I believe it's (A). It will double.
Explanation:
Charles law states that the volume of an ideal gas is directly proportional to the absolute temperature at constant pressure. (Charles's law).
The pressure, volume, and temperature are the main factors of the ideal gas. At constant pressure when the temperature is doubled, then volume also gets doubled. Thus, option A is correct.
What is an ideal gas law?The ideal gas law has been known as the law that relates the pressure, volume, moles, and temperature of the gas particles. The relationship between the volume and the temperature of the gas was given by Charle's law.
According to Charle's law, the volume and the temperature have been known to have direct relation when the factor of pressure is said to be constant. If they exhibit the direct relation then doubling the temperature will double the volume of the gas.
Therefore, option A. the doubling of temperature doubles the volume is correct.
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Write a balanced net ionic equation for the following reaction. Show your work. (3 points) upper B a upper C l subscript 2 baseline left-parenthesis a q right-parenthesis plus Upper H subscript 2 baseline upper S upper O subscript 4 baseline left-parenthesis a q right-parenthesis right-arrow upper B a upper S upper O subscript 4 baseline left-parenthesis s right-parenthesis plus upper H upper C l left-parenthesis a q right-parenthesis
Na₂ So₄(aq) + Ba (No₃)₂ (aq) → 2NaNo₃ (aq) + Baso₄ (s)
The complete ionic equation is,
2 Na⁺ (aq) + so₄²⁻ (aq) +. Ba²⁺ (aq) → 2 Na⁺(aq) + 2 No₃⁻ (aq) + Baso₄ (s) +2No₃⁻ (aq)
Canceling the same ions on both sides we get the net ionic equation as,
Ba²⁺ (aq) + so₄²⁻ (aq) → Baso₄ (s).
A net ionic equation is a chemical equation that describes only the elements, compounds, and ions directly involved in a chemical reaction. A net ionic equation is a chemical equation of a reaction that lists only the species involved in the reaction.
The net ionic equation is commonly used in acid-base neutralization reactions, double displacement reactions, and redox reactions. Break down all soluble ionic compounds into their respective ions and write the ionic equations. Each ion should be labeled with its charge and (aq) to indicate that it is in solution. A factor is used to indicate the number of each ion present.
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A chemistry student produces 1.45 grams of silver during an experiment by reacting
2.45 grams of silver nitrate with excess copper wire. The species of copper that is formed from this single replacement reaction is copper (Il) nitrate.
A. Calculate the students theoretical yield for this experiment.
B. Determine the percent yield for the student's experiment.
C. How would you rate the student's results, most excellent or poor?
D. Identify the reactant that would be left over upon the reaction reaching completion.
A single substitution reaction a strip or zinc metal is added to a copper(II) nitrate solution. Copper will be pushed out of the solution by zinc. As a result, zinc nitrate, Zn(NO3)2, and copper metal, Cu, produced.
What is a substitution reaction reaction example?An illustration of a single substitution reaction is the reaction of potassium (K) and water (H2O). In the process, hydrogen gas (H2) is released and potassium hydroxide (KOH), a colourless solid chemical, formed. 2K + 2H2O 2KOH + H is the reaction's equation.
What other names are given to copper II nitrate?This page explains the Copper II Nitrate formula, sometimes referred to as Cupric nitrate or Copper Dinitrate formula. This inorganic substance exists as a solid with blue crystals. Cu(NO3)2 is the chemical formula for Copper II Nitrate.
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A sample of an element has two naturally occurring isotopes. One isotope has a mass of 24.02 amu and makes up 49.4% of the sample. The other isotope has a mass of 28.02 amu. What is the average atomic mass for this element
One isotope has a mass of 24.02 amu and accounts for 49.4% of the total mass of the sample. The mass of the other isotope is 28.02 amu. The average atomic mass for this element is 25.99 amu.
To calculate the average atomic mass of the element, we need to use the relative abundance and mass of each isotope.
Let's call the mass of the first isotope x. We know that this isotope makes up 49.4% of the sample, so the relative abundance of the second isotope (with mass 28.02 amu) is 100% - 49.4% = 50.6%.
Now we can set up an equation to solve for x:
(0.494)x + (0.506)(28.02) = average atomic mass
Plugging in the values:
0.494)(24.02) + (0.506)(28.02) = average atomic mass
11.87188 + 14.16712 = average atomic mass
26.039 amu = average atomic mass
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las diferencias fundamentales del entramado urbano antes y despues de 1750
Answer:?
?Explanation:
What is the range of accuracy for food temperature?.
Answer:
+ 2 degrees Fahrenheit
Explanation:
a 1.16 mol sample of an element has a density of 13.55 g/cm3. if the sample occupies a volume of 17.17 cm3, what is the molar mass of the element?
To determine the molar mass of the element, we will use the formula:$$\mathrm{Molar\ mass}=\frac{\mathrm{Density}}{\mathrm{Moles}/\mathrm{Volume}}$$Given:$$\mathrm{Density}=13.55\:\mathrm{g/cm^3}$$$$\mathrm{Moles}=1.16\:\mathrm{mol}$$$$\mathrm{Volume}=17.17\:\mathrm{cm^3}$$.
Given that, density = 13.55 g/cm3molar mass = ?mass of sample = density x volume$$\therefore \ \text{mass of sample} = \mathrm{density} \times \mathrm{volume}$$$$\Rightarrow\mathrm{mass} =13.55 \:\mathrm{g/cm^3} \times 17.17 \:\mathrm{cm^3}=232.7315\:\mathrm{g}$$Now, Molar mass is given by,$$\mathrm{Molar\ mass}=\frac{\mathrm{Density}}{\mathrm{Moles}/\mathrm{Volume}}$$$$\Rightarrow\mathrm{Molar\ mass}=\frac{13.55\:\mathrm{g/cm^3}}{1.16\:\mathrm{mol}/17.17\:\mathrm{cm^3}}=103.1\:\mathrm{g/mol}$$Therefore, the molar mass of the element is 103.1 g/mol.
The given information is about the element's sample. The formula for molar mass is used to find out the unknown molar mass of the given element.
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NADPH inhibits the ____ pathway
NADPH inhibits the pentose phosphate pathway
NADPH is a product of the pentose phosphate pathway, which generates NADPH and ribose-5-phosphate.
NADPH is a coenzyme that plays a vital role in many metabolic processes, including the pentose phosphate pathway.
Therefore, it is incorrect to say that NADPH inhibits the pentose phosphate pathway. Instead, NADPH is a key participant in this pathway, where it is produced and utilized to synthesize nucleotides, amino acids, and fatty acids, and to protect cells against oxidative stress
When there is an excess of NADPH, it can inhibit the pentose phosphate pathway through feedback inhibition, thus reducing the production of NADPH. This is important to maintain a balance of NADPH and other metabolites in the cell.
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What makes the periodic table arrangement so unique and useful?
Which amino acid is always the first one to be incorporated into a newly made protein? this is because of the identity of the only start codon.
Methionine is always the first one to be incorporated into a newly made protein followed by identification of the only start codon on mRNA.
Amino acids are transferred by initiator tRNAs to the mRNA strand by the process of translation. Each tRNA has an anticodon, which is a group of three nucleotides that pairs with an appropriate mRNA codon to form a stable bond. The amino acid that the codon specifies is carried by the opposite end of the tRNA. AUG is one codon that serves as a "start" signal to initiate translation (it also specifies the amino acid methionine).
All newly created proteins have methionine as the first amino acid at their N-terminal end, the end of a protein that is synthesised initially, because the initiator tRNA always carries this amino acid (in bacteria, a modified version of methionine called formylmethionine is employed).
Transfer RNAs, or tRNAs, are molecules that read an mRNA's codons sequentially (from the 5' end to the 3' end) during translation.
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Osmosis is the process that H2O moves from where osmolality is low to where osmolality is high across a membrane that is impermeable to solutes. Osmolality is the number of solute particles per kg water. The osmolality of a cell is typically 300 mOsm. An isotonic solution would have the same osmolality as the cell, a hypertonic solution would have higher osmolality than the cell, and a hypotonic solution would have lower osmolality than the cell.
Effect of isotonic solution on cell shape:
Work in pairs, obtain a slide and coverslip. Add a drop of bovine blood to the slide, smear with a toothpick, and cover with a coverslip.
Examine under microscope (40x objective). Red blood cells are very pale. Closing the iris diaphragm can increase the contrast. Note the shape of red blood cells.
Add a drop of 0.9% NaCl or 5% dextrose to the edge of coverslip. As the fluid moves in under the coverslip, cells close to this edge are now soaked in this solution. Did these red blood cells change their shapes? Explain why.
Dispose the slide into the Glass box.
Effect of hypertonic solution on cell shape:
Obtain a new slide and coverslip. Repeat the above steps 1-2.
Add a drop of 25% NaCl to the edge of coverslip. As the fluid moves in under the coverslip, cells close to this edge are now soaked in this solution. Did these red blood cells change their shapes? Explain why.
Dispose the slide into the Glass box.
Effect of hypotonic solution on cell shape:
Obtain a new slide and coverslip. Repeat the above steps1.
Add a drop of distilled water to the edge of coverslip. As water moves in under the coverslip, cells close to this edge are now soaked in water. Did these red blood cells change their shapes? Explain why.
Dispose the slide into the Glass box.
In the provided activity, you are observing the effects of different solutions on the shape of red blood cells. Let's go through each effect and explanation:
Effect of isotonic solution on cell shape:
When an isotonic solution, such as 0.9% NaCl or 5% dextrose, is added to the edge of the coverslip, the red blood cells will not change their shape significantly. An isotonic solution has the same osmolality as the cell, meaning the concentration of solutes in the solution is similar to that inside the cell. As a result, there is no net movement of water across the cell membrane, and the cells maintain their original shape.
Effect of hypertonic solution on cell shape:
When a hypertonic solution, such as 25% NaCl, is added to the edge of the coverslip, the red blood cells will undergo a change in shape. A hypertonic solution has a higher osmolality than the cell, meaning the concentration of solutes in the solution is higher than that inside the cell. In this case, water will move out of the red blood cells through osmosis, from an area of lower osmolality (inside the cells) to an area of higher osmolality (the hypertonic solution). The loss of water causes the cells to shrink and become crenated or wrinkled.
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6.36 g of glucose were dissolved in 500 ml of water. Find the molality of glucose in the solution.how is this calculated
Answer:
Molality of glucose is 7×10⁻¹⁰ mol/Kg
Explanation:
\(Molality = \frac{Moles of solute}{Kg of Solvent}\)
you need to do 2 things before use this formula:
1. Transform 6.36 g of glucose into moles
2. Transform 500 mL of water into grams
1. To transform 6.36 g of glucose into moles, we need to know the molar mass of glucose. The molecular formula of glucose is C₆H₁₂O₆
Molar Mass of:
O = 16
H = 1
C = 12
So, to calculate the molar mass of glucose:
(6x12)+(12x1)+(16x6) = 72 + 12 + 96 = 180 g/mol
Now:
6.36 g of glucose --- x moles
180 g of glucose --- 1 mol
x = 6.36/180
x = 0.035 moles
2. First, transform 500 mL into L = 0.5 liters.
To transform 500 mL of water into grams we need to know the density of the water. It is 997 Kg/m³.
liters to m³:
1 liter --- 0.001 m³
0.5 --- x
x = 5×10⁻⁴ m³
997 Kg --- 1 m³
5×10⁻⁴ --- x
x = 5×10⁻⁷ Kg
Now: 0.035 moles/5×10⁻⁷ Kg =
Molality of glucose is 7×10⁻¹⁰ mol/Kg
Why is productivity greatest near the ocean’s surface
Answer:
Sunlight penetrates the top layers of the ocean, so photosynthesis occurs there.
Explanation:
Oxygen and chlorine gas are mixed in a container with partial pressures of 0.53 atm and 0.639 atm, respectively. What is the total pressure inside the container (in atm)?
Answer:
1.17 atm
Explanation:
Calculation to determine the total pressure inside the container (in atm)
Using this formula
Total pressure = Partial pressure 1 + Partial pressure 2
Where,
Partial pressure 1=0.53 atm
Partial pressure 2=0.639 atm
Let plug in the formula
Total pressure = 0.53 atm + 0.639 atm
Total pressure = 1.17 atm
Therefore the total pressure inside the container (in atm) is 1.17 atm
ASAAAP HURRRY PLS!!!
Where in aerobic respiration is the largest amount of energy produced?
A)
At the beginning of the cycle, when glucose is oxidized to pyruvate.
B)
At the end of the electron transport chain, when oxygen is reduced.
C)
At the end of the electron-transport chain, when oxygen is oxidized.
D)
At the beginning of the electron transport chain, when oxygen is oxidized.
If an object weighs 2,354 N on Earth, what is the objects mass?
Answer:
4.0 kg
Explanation:
M = W/g = 39.2/9.8 = 4.0 kg
sorry if its wrong-
20) When ice melts, what happens to the water molecules?
Answer:
start moving faster
Explanation:
i would guess because they slow down our freeze when they get cold
A hot metal plate at 150°C has been placed in air at room temperature. Which event would most likely take place over the next few minutes? Molecules in both the metal and the surrounding air will start moving at lower speeds. Molecules in both the metal and the surrounding air will start moving at higher speeds. The air molecules that are surrounding the metal will slow down, and the molecules in the metal will speed up. The air molecules that are surrounding the metal will speed up, and the molecules in the metal will slow down.
Answer:
last option is correct.
Air molecules surrounding the metal will speed up and metal molecules will slow down
Explanation:
the metal cools when placed in room temperature air which causes surrounding air to heat up.
the speed of molecules slow down for any material which cools and molecules speed increases when any material heats up
The number is (400). Type the number of significant figures.
one use for an electromagnet?
Answer:
It is used in induction of electric current through electromagnetic induction.
Atmos of given elements will have the same mass true or false .
True because,
Atoms of different elements have different masses because different atoms have a different number of subatomic particles i.e neutrons, protons and electrons.
Atomic mass of an element cannot be same as the mass of a single atom of that element because by investigation of mass spectrometry it was found that many naturally occurring elements exist in the form of two or more isotopes. Isotopes are the atoms of the same elements having the same atomic number containing the same number of protons but the different number of neutrons and because of this, it possesses different mass number. So, the observed atomic mass of the atom of an element is the average atomic mass of the elements. Average of masses of a different isotope of the same element is taken and then the atomic mass of the element is decided.
A formula to calculate average atomic mass:-
Example:-
Lithium exists in nature in the form of two isotopes Li−6 and Li−7 with atomic masses 6.0151u and 7.0160u and the percentages 7.59 and 92.41 respectively.
Solution:- Average atomic mass of Li
[Atomic mass of Li−6× percentage + Atomic mass of Li−7× percentage]/100
=
100
6.015u×7.59+7.0160u×92.41
=6.939u
Hence, the atomic mass of an element is not the same as the mass of a single atom of that element; Atomic mass is average of isotopic mass o atoms.
Answer:
TRUE BRUHHHH maybe
Explanation:
TRUE BRUHHHHHHHGGGHHHGHGHGHGHHHHHHHHHHHHHHHHHHHHHHH
Balancing Chemical equations
20.
Answer:
Count each type of atom in reactants and products. ...
Place coefficients, as needed, in front of the symbols or formulas to increase the number of atoms or molecules of the substances. ...
Repeat steps 1 and 2 until the equation is balanced
Explanation:
Step 1: Coefficients Versus Subscripts. When approaching a chemical equation, it is important that you understand the difference between coefficients and subscripts. ...
Step 2: Sum the Atoms. ...
Step 3: Balance the First Element. ...
Step 4: Repeat for the Other Elements. ...
Step 5: Tips.
Answer:
2C6H6 + 15O2 = 12CO2 + 6H2O
Explanation:
This is a combustion reaction meaning a substance (in this case Benzene or C6H6) reacts with oxygen gas (O2) and releases energy in the form of light and heat.
so Benzene (C6H6) and Dioxygen (O2) are reactants meaning they are on the left side of the equation
Carbon Dioxide (CO2) and Water (H2O) are the products meaning they are on the right side of the equation
To balance an equation you need an equal amount of each element/compound/polyatomic on each side of the equation
so 2C6H6 + 15O2 = 12CO2 + 6H2O:
(2•6) = 12 C = (12•C) = 12 C ~ 12C = 12C
(2•6) = 12 H = (2•6) = 12 H ~ 12H = 12H
(15•2) = 30 O2 = [(12•2) • (6•1)] = 30 O2
~ 30 O2 = 30 O2
so the equation is now balanced
feel free to ask if you have any other questions
hope this helps :)
What volume of O2 at STP is required to oxidize 8.0 L of NO at STP to NO2? What volume of NO2 is produced at STP?
The balanced equation of the reaction is:
\(O_2 + 2 NO\) ⇒ \(2 NO_2\)
Every gas occupied 24 L in one mole in r.t.p. according to the equation:
\(24 \times n = V\)
8/24=0.333 mol
2 moles of NO makes 2 moles of \(NO_2\\\) so
0.333*24=8L
PLEASE HELP ASAP for 75 points
A 5.00 L flask is charged with 63.45 grams of I₂ and 2.5 atm of F₂ at 25°C. The flask is heated to 100°C until one of the reagents is completely consumed. What will be the total pressure (in atm) of the final products in the flask at 100°C?
Based on the calculations, the total pressure of the final products is equal to 1.76 atm.
How to calculate the total pressure (in atm)?From the information provided about this chemical reaction, we can logically deduce the following parameters:
Volume, V = 5.00 L.Mass, m of I₂ = 63.45 grams.Pressure, P of F₂ = 2.5 atm.Initial temperature, t₁ = 25°C.Final temperature, t₂ = 100°C.Next, we would write the properly balanced chemical equation for this chemical reaction:
I₂ + 5F₂ ⇒ 2IF₅
Also, we would determine the number of moles of each atom of I₂ and F₂:
\(Number \;of \;moles = \frac{mass}{molar\;mass}\)
Substituting the given parameters into the formula, we have;
Number of moles = 63.45/253.8
Number of moles = 0.25 moles.
Assuming I₂ were limiting, we would need:
5 × 0.25 = 1.25 moles of F₂.
For fluorine gas, we have:
PV = mRT/MM
Mass, m = PVMM/RT
Mass, m = 2.5(5.00)(38)/(0.0821 × 298)
Mass, m = 475/24.4658
Mass, m = 19.42 grams.
Number of moles = 19.42/38
Number of moles = 0.51 moles.
The total number of moles = 0.25 + 0.51 = 0.76 mol.
For the mole fraction of I₂, we have:
Mf = 0.25/0.76
Mole fraction = 0.33.
For the mole fraction of F₂, we have:
Mole fraction = 1 - 0.33 = 0.67.
Next, we would determine the total pressure of the two elements by applying Dalton's law:
Total pressure = 0.33 × 0.27 + 0.67 × 2.5
Total pressure = 1.76 atm.
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10. A community currently uses coal to produce electricity. What would be an
advantage of using nuclear energy instead? *
a. Uranium is safer than coal
b. Nuclear energy produces no waste
c. Uranium deposits are more abundant than coal
d. Nuclear energy does not produce greenhouse gases
Answer:
c
Explanation:
I'm pretty sure it's C. If it is not then it might be d
How many significant figures are there in
9.710 x 10-4
Answer:
91.3
2 Significant figures
Answer:
The values have 2 significant numbers ╮( ̄ω ̄;)╭
why does the hydrogen gas need to flow continuously for a while before starting the heating process?
In the laboratory, hydrogen gas is used as fuel for various purposes, including heating. In order to start the heating process, it is necessary to allow the hydrogen gas to flow continuously for a while. This is because there may be air or other gases present in the hydrogen gas pipeline that can affect the heating process.
When the hydrogen gas is allowed to flow continuously for a while, the air or other gases are purged from the pipeline, which improves the quality of the hydrogen gas. This ensures that there is no interference with the heating process, which could otherwise lead to inaccurate results.The continuous flow of hydrogen gas is essential because if it is not allowed to flow for a while, air or other gases can cause damage to the burner or other equipment used for heating. The air or other gases can cause an explosion, which can result in severe injury or death.In conclusion, the hydrogen gas needs to flow continuously for a while before starting the heating process to remove any air or other gases from the pipeline. This improves the quality of the hydrogen gas, ensures accurate results, and prevents damage to the equipment. It is important to follow safety protocols when using hydrogen gas to prevent any accidents.For such more question on heating process
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am i right??? this is graded and no links plz!!!!
Answer:
Yes! You are correct
Explanation:
The higher the frequency of light, the higher its energy. .