You are working in a bacterial genetics laboratory. You are studying the biosynthetic pathway for the amino acid arginine in e. Coli and have two mutant strains: arge, and argg that were generated by transposon mutagenesis. You need to perform an experiment with these two arginine biosynthesis mutants, and so you first streak them from your stock culture onto la so that they have access to arginine. Once the strains have grown on the la plate, you streak individual colonies of both mutants onto m9, m9 ornithine, and m9 l-argininosuccinate plates that you purchased from a reputable supplier. As expected, the arge mutant cannot grow on m9 unless ornithine is supplemented. Unexpectedly, the argg mutant can grow on all three plates, whether l-argininosuccinate is supplemented or not. What is the most plausible explanation for these unexpected results?

Answers

Answer 1

The most plausible explanation for the unexpected results is that the argg mutation occurs downstream of the arge mutation in the arginine biosynthetic pathway. The arge mutation has a defect in the step converting ornithine to citrulline, which can only be restored by supplementing ornithine to the growth media.

However, the argg mutant can grow on all three plates without the need for L-argininosuccinate supplementation. Arginine biosynthesis in E. coli is a multistep pathway. It begins with the enzyme N-acetylglutamate synthase (encoded by the argA gene), which catalyzes the formation of N-acetylglutamate.

In the next step, N-acetylglutamate kinase (ArgB) phosphorylates N-acetylglutamate to form N-acetylglutamyl phosphate. N-acetylglutamyl phosphate is then converted to ornithine by ornithine transcarbamylase (argF) and ornithine is further converted to citrulline by argininosuccinate synthase (argG) followed by argininosuccinate lyase (argH) to form arginine.

Therefore, it can be concluded that the arge mutation occurs at the step converting ornithine to citrulline while the argg mutation occurs at the step converting argininosuccinate to arginine. The arge mutation prevents the conversion of ornithine to citrulline while the argg mutation does not affect the conversion of argininosuccinate to arginine. Thus, it can grow on all three plates without the need for L-argininosuccinate supplementation.

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Related Questions

PLSSS HELP IF YOU TURLY KNOW THISS

PLSSS HELP IF YOU TURLY KNOW THISS

Answers

Answer:

C. Soil

Explanation:

I'm pretty smart

a) why do you think that the concentration at which microtubules begin to form (the critical concentration) is different in the two experiments?

Answers

The difference in the critical concentration at which microtubules begin to form in the two experiments could be attributed to variations in experimental conditions, protein sample purity, stabilizing or destabilizing factors, or differences in the experimental setup.

The reason the concentration at which microtubules begin to form (the critical concentration) is different in the two experiments could be due to several factors. Some possible factors include:

1. Variations in experimental conditions: Differences in temperature, pH, or buffer composition could affect the critical concentration.

2. Purity of the protein sample: Different levels of purity in the tubulin protein samples used in the experiments might influence the critical concentration.

3. Presence of stabilizing or destabilizing factors: Various proteins or molecules can interact with microtubules and affect their stability. If the two experiments have different concentrations or types of these factors, it may cause differences in the critical concentration.

4. Differences in the experimental setup: Different methods of measurement or data analysis might result in discrepancies in the observed critical concentration.

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Chemicals released into the space between nerve cells that bind to receptors are calledQuestion options:A) action potentials.B) neurochemicals.C) neurotransmitters.D) platelets.

Answers

The correct answer is C) neurotransmitters. Neurotransmitters are neurochemicals that work between neurons' spaces. As the name describes, they transmit information between nerve cells.

After a long soccer practice, Jack felt his pulse increase. As he cooled down, his pulse began to slow. Since he’d been studying the circulatory system in science class, he knew why his pulse slowed. Jack knows that the blood vessels carry oxygen to the body and pick up carbon dioxide. Drag the words to complete the sentence.

Answers

Explanation: so during his practice hours  to fulfil his body's oxygen requirement  a person's pulse increases and hence meet up the body's oxygen demand.  

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Jasmine went to the tide pool. She took pictures of some animals. How can you tell which tide zone the animals live in?

Answers

Answer:

food and population

Explanation:

Different animals eat different things for example a sea urchin eats seaweed so if she took a picture of a urchin you would find it in the one that has the most vegetation. the urchins try to space out so look how many animals are in the picture. I hope that helps ;)  

Answer:

food and population :)

Explanation:

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What is the similarity between a Pine tree and a daisy 4th grade

Answers

The similarities between a pine tree and a daisy are that they both contain plant cells and therefore rely on photosynthesis to sustain themselves.

What do these organisms have in common?

The first similarity is their classification as plants. Both organisms contain plant cells, along with plant-specific organelle that allows them to complete the functions they rely on.

One such function is photosynthesis, a process by which plants turn sunlight into nutrients needed to survive. The similarities between a pine tree and a daisy are that they both contain plant cells and therefore rely on photosynthesis to sustain themselves.

Therefore, The similarities between a pine tree and a daisy are that they both contain plant cells and therefore rely on photosynthesis to sustain themselves.

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The first stage in the production of atp via the oxidation of glucose molecules is referred to as:________

Answers

The first stage in the production of ATP via the oxidation of glucose molecules is referred to as glycolysis.

Glycolysis is a metabolic pathway that occurs in the cytoplasm of cells and is the initial step in the breakdown of glucose to produce energy in the form of adenosine triphosphate (ATP). It is a central pathway in cellular respiration and occurs in both aerobic (oxygen-present) and anaerobic (oxygen-absent) conditions.

Glucose Activation: Glycolysis begins with the activation of glucose, a six-carbon sugar molecule. Through a series of enzymatic reactions, glucose is phosphorylated twice, resulting in the formation of fructose-1,6-bisphosphate. Cleavage: Fructose-1,6-bisphosphate is then cleaved into two three-carbon molecules called glyceraldehyde-3-phosphate (G3P). This step is facilitated by the enzyme aldolase.

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Reeds, sedges, and cattails are an indication of which type of native vegetation?

A Forest

B Prairie

C Marsh

D Transition

Answers

Answer: c) Marsh

Explanation:

Please help me! and please don't just take the points
True or False: Genes and chromosomes are found in every cell.
True or False: ALL genes that are inherited show up in the offspring.
True or False: Characteristics you see on an organism will ALWAYS be the same as the parent.

Answers

Answer:

1.every cell in the human body contains chromosomes. Genes are found in ALMOST every cell of the body.

2.True

3.false

Explanation:

brief summary of conservation of forests & wildlife

least 10 page

Answers

With the advent of industrialization, several trees have been cut at an alarming rate for raw materials and various other purposes. This felling of trees can be regulated by selective cutting, clear-cutting and shelterwood cutting.

Forest fires are one of the common causes of loss of forests. Sometimes the forest land is set on fire to make the land available for commercial purposes. Once cleared, there can be no vegetation. Natural forest fires are also responsible for the destruction of huge forest covers. Latest fire fighting techniques should be adopted to conserve the forest. However, forest fires are an important part of the ecosystem and it helps replenish nutrients in the soil from dead and decaying matter.

More trees should be planted to increase the forest cover. Trees should be selected according to the geographical conditions of a particular region and proper care should be taken during the growth of trees.

Prevention of exploitation of forestry and forest products is necessary for the conservation of forest.

The existing forests should be protected from diseases by spraying chemicals, antibiotics or development of pest-resistant strains of trees.

The S-P interval of seismic waves recorded at a seismometer is 8 minutes. The equation for determining this distance to the
epicenter is
S-P interval=0.001613 x distance to epicenter
Approximately how far away is the earthquake's epicenter from the seismometer?
less than 1 km
8.km
O 500 km
5,000 km

Answers

According to the equation of the seismic waves, the earthquake's epicenter from the seismometer is approximately 5000km.

What is a seismic wave?

A seismic wave can be described as a vibration generated by an earthquake, explosion, or similar energetic source and propagated within the Earth or along its surface.

From the question, we have that

S-P interval = 0.001613 x distance

therefore distance =  s-p interval/0.001613

8/0.001613 = distance to epicenter

distance to epicenter =8÷0.001613

=4959.702

approximately 5000km.

Seismographs are devices that record the amplitude and frequency of seismic waves and yield information about the Earth and its subsurface structure.

There are several different kinds of seismic waves, and they all move in different ways.

The two main types of seismic waves are body waves and surface waves. Body waves can travel through the Earth's inner layers, but surface waves can only move along the surface of the planet like ripples on water. Earthquakes send out seismic energy as both body and surface waves.

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1. What is the best most obvious observation from this photo that this is a pond or lake
2. What evidence is there of the abiotic factor of sunlight in this ecosystem?
3. What producer supports the food chains in this ecosystem?
4. What would this ecosystem look like if nutrias were present?
5. What type of human activity could negatively impact this ecosystem? how?

1. What is the best most obvious observation from this photo that this is a pond or lake2. What evidence

Answers

Answer:

how a freshwater ecosystem, such as a pond, can change into a terrestrial ecosystem.​

Explanation:

PLEASE HELP ANSWER FAST WILL MARK BRAINLIEST AND GIVE 20 POINTS
Susana heats up a sample of red crystals. While the sample is being heated, a gas is released, and a blue powder is left after the heating. Is the sample of red crystals an element or a compound? Explain your answer

Answers

Answer:

its a compound

Explanation:

because it was broken down into a gas and blue powder, elements cant be broken into simpler substances.

what are the main conditions the cell checks for before passing the s/g1 checkpoint? the dna is intact; growth hormone is present in the environment. dna replication is complete and the dna is intact. dna replication has begun and the cell has plenty of space. the dna is intact; the cell has plenty of nutrients and space. the cell has plenty of nutrients and space, and growth hormone is present in the environment.

Answers

he dna is intact; the cell has plenty of nutrients and spaceare the main conditions the cell checks for before passing the s/g1 checkpoint

At the S checkpoint, what cell conditions are monitored?

The correct replication of DNA during the S phase is one of the important activities controlled by the cell cycle checkpoint monitoring system. Even when all cell cycle regulators are completely functional, a tiny proportion of replication mistakes (mutations) are passed on to daughter cells.

The cell must pass the G1 checkpoint before moving on to the next phase of interphase. This requires the presence of cyclin proteins as well as the correct biological circumstances. If the cell survives the checkpoint, it will enter the S phase, which is when DNA replication occurs.

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All the following are usually recessive traits in humans EXCEPT: Your answer: Dark hair Colorblindness Straight hair Left-handedness

Answers

Answer:

dark hair

Explanation:

What are the products of the chemical reaction of cellular respiration?.

Answers

Answer:

H2O, ATP, and CO2

Explanation:

Oxygen and glucose are converted to water, carbon dioxide and ATP. ATP  is energy for animal cells.

What specializations might allow one tissue to produce a hormone, and another to facilitate nutrient exchange? is it possible for the same organ to serve both these functions?.

Answers

Differential gene expression patterns allow one tissue to produce a hormone, and another to facilitate nutrient exchange. The same organ can serve both functions because it can synthesize different proteins.

Diferential gene expression

Gene expression refers to the differential process by which different genes are expressed (transcribed and then translated) in different cell types.

Gene expression enables different functions due to the production of different types of proteins which may have both enzymatic and structural functions.

A specialized cell type or organ can synthesize different proteins and also generate other molecules (either enzymes or structural proteins) due to the process of cell differentiation.

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Self-contained, living structures that join together to form tissues are called.

Answers

Answer:

Cells

Explanation:

Identify the steps of the lysogenict

Answers

Answer:

cycle:

1. Attachment: A bacteriophage attaches to the surface of a bacterial cell.

2. Entry: The viral DNA is injected into the bacterium and integrates into the host chromosome.

3. Prophage: The integrated viral DNA is known as a prophage and is replicated along with the bacterial chromosome.

4. Replication: The prophage replicates along with the bacterial DNA during cell division.

5. Induction: A trigger, such as stress or UV radiation, causes the prophage to leave the bacterial chromosome and enter the lytic cycle.

6. Lytic cycle: The viral DNA begins replicating and producing new phages, which lyse the host cell and release new viruses.

7. Release: The newly produced phages are released into the environment to find other bacterial cells to infect.

Note: The lysogenic cycle can sometimes be followed by the lytic cycle, in which the virus uses the host cell to produce new viruses, ultimately leading to lysis of the host cell.

What contains the greatest concentration of lactic acid?

Answers

Answer: Your answer should be:

C. muscle cells during vigorous exercise

Explanation:

Lactic acid is produced in your muscles and builds up during intense exercise.

predict what the results would be if mrna were radioactively labeled instead of polypeptides. give reasoning to support your prediction.

Answers

If mRNA were radioactively labeled instead of polypeptides, the radioactivity would be detected in the cytoplasm, indicating the location of mRNA synthesis, transportation, and localization within the cell.

If mRNA were radioactively labeled instead of polypeptides, the results would show that the radioactive label is primarily located in the cytoplasm of the cell, specifically in the ribosomes, which are the site of protein synthesis. This is because the mRNA molecule serves as the template for the synthesis of the polypeptide chain.

As the ribosome reads the mRNA, it translates the genetic code into an amino acid sequence, resulting in the production of a polypeptide chain. Therefore, radioactively labeled mRNA would reveal the location of active protein synthesis in the cell.

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An organism's body weight is a balance between: the energy carried in the food we eat and the energy burned in digesting that food. the energy burned during growth, reproduction, and movement and the energy carried in the food we eat. the energy an organism spends on growth and the energy an organism invests in reproduction. the energy stored as lipids within the body and the energy burned during all activities. the energy carried in the food we eat and the energy burned by our basal metabolic rate.

Answers

Answer:

the energy burned during growth, reproduction, and movement and the energy carried in the food we eat.

Explanation:

A good health can be defined as the state of well-being in which all of the components of an individual's health are in balance. These state of well-being comprises of six (6) important components and they are; physical, spiritual, emotional, environmental, social and mental or intellectual. In order to have a good health, physicians and medical professionals advise individuals to engage in physical activity such as an aerobic exercise.

An aerobic exercise refers to all forms of physical activity that an individual engages in, in order to increase the heart rate (from low to high intensity) in response to the level of oxygen required by any activated or working muscle in the human body.

Some examples of aerobic exercise (cardiofitness activity) includes cycling, swimming, jogging, walking, skiing etc.

Generally, fitness experts usually advises everyone to judiciously engage in cardiofitness activities or exercises so as to improve well-being, body mass index (BMI), wellness or heart health.

Hence, the body weight of an organism is simply a balance between the energy burned during growth, reproduction, and movement and the energy carried in the food we eat.

This ultimately implies that, an organism's body weight is the difference between the energy that is taken in and the energy expended or burn throughout body activities.

the skin of a shark is covered with small, spiny projections called:

Answers

Dermal scales, also known as dermal denticles or placoid scales, are small, spiny projections that cover the skin of a shark. Here option A is the correct answer.

These unique structures provide several advantages to sharks. Each dermal scale consists of a hardened, tooth-like structure embedded in the shark's skin, with the pointed end protruding outward. The scales are composed of dentine, a tough and mineralized tissue, and are covered by a layer of enamel-like material.

The presence of dermal scales enhances the shark's hydrodynamics by reducing drag as water flows over the skin. The scales create a rough surface, which helps in reducing turbulence and increasing swimming efficiency.

They also provide protection against abrasion and parasites. The shape and arrangement of dermal scales vary among different shark species, reflecting their specific adaptations and lifestyles.

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Complete question:

What are the small, spiny projections that cover the skin of a shark called?

a) Dermal scales

b) Dorsal fins

c) Gill slits

d) Shark teeth

if a student analyzes the sounds and syllables in a word, it is easier for the student to store the word in semantic memory.
A. True
B. False

Answers

Answer:

False

Explanation:

If a student analyzes the sounds and syllables in a word, it is easier for the student to store the word in semantic memory.

The statement is false. The correct option is B.

Analyzing the sounds and syllables in a word primarily involves phonological processing, which is related to the phonetic and phonological aspects of language.

Semantic memory, on the other hand, is responsible for storing the meanings and concepts associated with words and knowledge about the world.

While phonological processing can aid in the initial encoding and retrieval of words, it is not directly linked to the storage of words in semantic memory.

Semantic memory is more closely associated with the understanding and comprehension of word meanings.

Thus, the correct option is B.

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pls hurry im in a test !!
Which option describes the grade of egg most appropriate for the following scenario?

Debbie, a housemother in a fraternity house, cooks breakfast on Sunday mornings for the 25 residents, with omelets made to order.


B
A
AA
Jumbo

Answers

The grade that best fits the situation is jumbo eggs. Jumbo eggs, which are the largest grade of eggs and will supply the most egg for an omelette, account for this.

Jumbo eggs also frequently have the most yolk, which makes them perfect for omelettes. They are also simpler to manage while cooking for a big group of people because of their larger size.

It's important to remember that the final decision about the size of the eggs in an omelette is one of personal preference. Some people might find it simpler to manage and prefer the flavour or texture of smaller or medium-sized eggs for their omelettes. Jumbo eggs would be the most sensible option, though, if the aim is to produce a big omelette that will feed a crowd.

Additionally, the proportion of yolk to egg white varies depending on egg size, which may have an impact on the taste and texture of the omelette.

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You discover a new species of insect. You learn that its gamete contain 4 chromosomes each and contain 20 pg of DNA. Given this information, what can you conclude about this organism's somatic cells? a They will contain 4 sister chromatids. b They will contain 40 pg of DNA during GO c They will contain 2 chromosomes during prophase of mitosis. d They will be haploid (2n)

Answers

 options a, b, and c are not accurate conclusions based on the given information.

The correct answer is d) They will be haploid (2n). Gametes are haploid cells, meaning they contain half the number of chromosomes as somatic cells. Since the insect's gamete contains 4 chromosomes, the somatic cells would have a diploid number of 8 chromosomes (2n = 8). The amount of DNA in the gamete is not necessarily indicative of the amount of DNA in the somatic cells, as somatic cells can undergo DNA replication and have varying amounts of DNA depending on the stage of the cell cycle.

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The paris agreement intends to direct money toward limiting climate change
and lowering greenhouse gas levels. which groups are likely to benefit from
the paris agreement? select the three correct responses.
o a. solar power companies
o b. hydroelectric dam engineers
c. petroleum companies
d. hydraulic fracking operators
e. geothermal reservoir drillers
please help.

Answers

Answer:a,b,

Explanation:

The groups which are likely to benefit from the Paris agreement are solar power companies, and hydroelectric dam engineers. Thus, option A and B are correct.

What is Paris Agreement?

The Paris Agreement deals with production of those types of energy which is clean and which don't emit (or emit very less) green house gasses, the root cause of climate change and global warming.The term climate change has to do with the alteration of weather patterns owing to the release of harmful gases commonly called greenhouse gases.

The main goal of the Paris Agreement is to strengthen the global response to the threat posed by climate change by limiting the rise in global temperature this century to well below 2 degrees Celsius above pre-industrial levels and to pursue efforts to further limit the temperature increase to 1.5 degrees Celsius.

Therefore, The groups which are likely to benefit from the Paris agreement are solar power companies, and hydroelectric dam engineers. Thus, option A and B are correct.

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A haploid cell that combines with another haploid cell during fertilizationa) ATP (adenosine triphosphate)b) chromosomec) crossing overd) gametee) genef) germ cellg) meiosish) mitosisi) translationj) transcription

Answers

The term used to describe a haploid cell that combines with another haploid cell during fertilization is "gamete." Here option D is the correct answer.

The term "haploid cell" refers to a cell that contains half the normal number of chromosomes in the genome. This type of cell is often involved in reproduction, as it combines with another haploid cell to form a zygote, which is the first cell of a new organism.

During fertilization, the haploid cells, called gametes, come together and their chromosomes combine to form a diploid cell, which has the full complement of chromosomes.

During fertilization, two haploid cells, usually an egg and sperm, combine to form a diploid zygote which has the full complement of chromosomes in the organism. In other words, a haploid cell is a cell that contains only one set of chromosomes while a diploid cell has two sets of chromosomes.

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1. It is the state of matter that is very compressible a. liquid b. solid c. gas d. Plasma NEED HELP GUYS

Answers

Answer:

C

Explanation:

In general, Gases are the most compressible and solids are the least compressible.

Answer:

In general, Gases are the most compressible and solids are the least compressible.

Explanation:

The main reason for this is the intermolecular forces which determine the physical state of a substance by altering the intermolecular spaces. hope this helps you :)

according to edward o. wilson, biodiversity . select one or more: a. cannot be conserved b. is a nonrenewable resource because extinction is irreversible

Answers

Edward O. Wilson is an American biologist and researcher who coined the term "biodiversity" in 1988. Biodiversity is defined as the variety of life on Earth at all levels of organization. According to Edward O. Wilson, biodiversity is a nonrenewable resource because extinction is irreversible.

In recent decades, there has been a growing concern about the rapid loss of biodiversity due to human activities such as habitat destruction, climate change, overexploitation of resources, pollution, and the introduction of invasive species. These activities have caused a significant decline in the number of species on the planet, with many facing the threat of extinction.

The loss of biodiversity has significant consequences for the health and well-being of ecosystems, as well as for human societies. Ecosystems provide essential services such as clean air and water, nutrient cycling, pollination, and pest control, all of which contribute to the health and productivity of human societies.

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HELP THIS IS DUE TODAY!! Why is the Renaissance seen as a rebirth?Why was the Renaissance important? what is the meaning of the poem there but for the grace by wislawa szymborska PLS HELP ASAP WILL GIVE BRAINLIEST! You've observed the following returns on Pine Computer's stock over the past five years: 10 percent, 11 percent, 18 percent, 19 percent, and 10 percent. a. What was the average real return on the companys stock? (Do not round intermediate calculations and enter your answer as a percent rounded to 2 decimal places, e.g., 32.16.)b. What was the average nominal risk premium on the companys stock? (Do not round intermediate calculations and enter your answer as a percent rounded to 1 decimal place, e.g., 32.1.) When jimi hendrix returned to america to perform at the monterrey pop festival, he returned as something else. What did he not return as?. An increase in government expenditure shifts the ..... curve to the Ltfen birini sein: a. IS/right b. LM/right C. LM/left d. Is /left Whole Foods has grown from a local small store to a large corporation. As a large company, Whole Foods has been able to make managerial decisions as a small store through the use of Multiple Choice decentralization. executive management. centralized authority. vertical hierarchy. corporate culture. Need answer as soon as possible, will give brainly if it's right Based on the activities that the following children are performing, place them in order from earlier to later stages of Piaget's theory of cognitive development. I need help with this question plsssThx Justin is buying a cell phone that had a wholesale price of$495. The cell phone was marked up 25%.What is the selling price of the cell phone? Jose sold half of his comic books and then bought 25 more. He now has 45. How many did he start out with? 4. Which stage of the Hero's Journey does Othello NOT complete?O The DepartureO InitiationO The Return why might people live in some areas and not others 16. Complete the following identity: A. tan 5x B. tan 2x + tan 8x C. 2 tan 5x tan 3x D. tan 5x cot 3x sin 2x + sin 8y cos 2x + cos 8y ? a circle rug measures 8ft across. what is the area of the rug [10 pts] how do you have to set up the control signals to transfer a numeric value from the main memory (ram) to the accumulator register on the brainless microprocessor? A bag contains colored tiles. 33 tiles are red 66 tiles are green 33 tiles are blueA tile will be randomly selected from the bag. What is the probability in decimal form that the tile selected will be green? HELP ME!!!!!! Read the excerpt from the story, "Never Say Never."Last week, his lifetime of confidence collided with catastrophe, and his confidence vanished.How does this statement help develop the plot?It foreshadows that Carlos will quit the talent show.It explains why Carlos had trouble singing at the mall.It introduces a flashback that explains Carlos's conflict.It helps to explain why Carlos won't go back to the mall. The bees swirledaround their hivebecause they werebusy at work. Metaphor or simile.