Why you must degas the solvents before they can be used as HPLC mobile phases? How to degas solvents?

Answers

Answer 1

Solvents used as HPLC mobile phases must be degassed before use to avoid introducing gas bubbles into the chromatographic system. Gas bubbles can cause fluctuations in pressure, flow rate, and peak shapes, ultimately leading to inaccurate and irreproducible results.

There are a few methods for degassing solvents, such as sonication, sparging with helium or nitrogen gas, or using a vacuum pump. Sonication involves placing the solvent in a container and using high-frequency sound waves to remove any dissolved gases. Sparging involves passing an inert gas through the solvent, which displaces any dissolved gases. Vacuum degassing involves placing the solvent under reduced pressure, which allows dissolved gases to escape. Regardless of the method used, it is important to ensure that the solvents are fully degassed before use to avoid any issues during HPLC analysis.
In conclusion, degassing solvents is an important step in preparing HPLC mobile phases to ensure accurate and reproducible results. The chosen method of degassing will depend on the specific solvent and equipment available.

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Related Questions



How does oil become a political issue?


1. People have to pay more taxes when there is a low supply of oil.

2. Healthcare availability is affected by the oil supply.

3. Some countries control the supply of oil to make more money.

4. The president of the country controls the oil supply.

Answers

Oil becomes a political issue because some countries control the supply of oil to make more money.

Oil becomes a political issue for many reasons such as its scarcity, the power of the oil-rich countries, and the international politics of oil. The answer option that best explains how oil becomes a political issue is that some countries control the supply of oil to make more money.

Oil is a valuable commodity in the world today, and many countries depend on it for their economic growth and development. Some countries that have vast oil reserves use the control of the oil supply as a bargaining chip to make more money. They limit the supply of oil to other countries, thereby creating an artificial scarcity, and as a result, drive up the price of oil. This is a form of political manipulation and can have far-reaching consequences.

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Explain in a three-paragraph essay the mechanics of how a battery works. How does the choice of metals used in a battery affect its performance? what specific metals work best?

Answers

A battery is a device that converts chemical energy into electrical energy through a process known as an electrochemical reaction.

How does a battery work ?

When a battery is connected to a circuit, the electrochemical reaction causes a flow of electrons from the anode to the cathode, generating an electric current that can power a device.

The metal chosen for the anode must be capable of losing electrons easily, while the metal chosen for the cathode must be capable of accepting electrons. The choice of metals can also affect the voltage and capacity of the battery, as well as its overall efficiency.

In general, the metals used in a battery should have a large difference in their electronegativity values, which determines how easily an atom can attract electrons. Common metals used in batteries include zinc, lithium, nickel, and cadmium.

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I'm not sure what to do here...

I'm not sure what to do here...

Answers

Answer:

Explanation:

As relative molecular mass of molecule increases its rate of diffusion decreases . Mr of HCl is 1+35.5=36.5 which is greater than Mr of NH3 which is only 14+3=17. As HCl is heavier it'll diffuse at a lower rate so travel less distance whilst NH3 will cross a greater distance as it diffuses faster so white solid appears closer to HCl that is approximately B.

Fill the blank.
If two organisms look very similar during their early stage of development, this is evidence that the organisms ___.

A. are not related
B. evolved from different ancestors
C. have exactly the same Dna
D. evolved from a common ancestor

Answers

D.
Hope this helps:)

Joshua observed the changes in a cake when it was baked in an oven.what best explains that he observed a chemical change?

Answers

What best explains that he observed a chemical change? He noticed the steam from the moisture rise in the oven

Answer:

He noticed the flour and other ingredients lose their unique properties.

Explanation:

I took the test and got it right

Jodie is studying the sleeping habits of various types of animals. She wants to know how long each type of animal sleeps during the day.

Jodie puts each of the animals in a cage with food, water, and light. However, she cannot stay to watch the animals all day.

Which of the following tools would be most helpful to Jodie's experiment?
A.
a tape recorder
B.
a video camera
C.
a metric ruler
D.
a triple beam balance

Answers

A. You want to check the sleeping schedule of the animals including the time.

An atom's electron configuration ends with 3p4. if another atom has seven more electrons, what would be the continuation of the electron configuration? 3p63d34s2 3p43d54s2 3p64s23d3 3p44s23d5

Answers

The continuation of the electron configuration is 3p6 4s2 3d3.

Electronic configuration ends up with = 3p4

So, here principle quantum number(n) =3

Atom which ends up with 3p4 configuration = S

Sulphur(s) = [Ne] 3s2 3p4

So atomic number of Sulphur (s) =16

After adding 7e in it -

Atomic number = 16+7 =23

Now atomic no. =23 (after adding 7 more electrons)

So electronic configuration with atomic no. =23

Atomic number (23) - [Ne] 3s2 3p6 4s2 3d3

Now electronic configuration will ends up with= 3d3

Electrons belong to the primary technology of the lepton particle's own family and are typically thought to be fundamental particles because they have no recognized additives  substructure.

For most realistic purposes, an electron is a structureless particle with an intrinsic angular momentum or spin. simply two numbers — the electron's mass and its electric price — gasoline the equations that describe its behavior. From this 'sensible electron' version, physicists constructed present-day microelectronics.

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How do you determine if you have a covalent bond?

Answers

Answer:

If the electronegativity difference (usually called ΔEN) is less than 0.5, then the bond is nonpolar covalent. If the ΔEN is between 0.5 and 1.6, the bond is considered polar covalent. If the ΔEN is greater than 2.0, then the bond is ionic.

Explanation:

Hope this helped :)

the main group elements on the periodic table are only in which orbitals?

Answers

Answer:  Abstract. The main group elements are classified as belonging to the s- and p-blocks in the periodic table. They range from gases such as fluorine and oxygen through nonmetals (e.g., boron, phosphorus, and sulfur) and semimetals (metalloids; e.g., germanium) to very reactive metals such as sodium and potassium.

Explanation:

a student was given a dye sample believed to contain several components. in an attempt to determine the identity of those components, she decided to analyze the mixture using tlc. the student used both 100% hexanes and 50% hexanes in ethyl acetate as eluting solvents and spotted known dyes for comparison. the following tlc chromatographs were obtained. which is the best solvent choice for this analysis?

Answers

The best solvent choice for this analysis will be 50% hexanes in ethyl acetate.

Ethyl acetate having a molecular weight of 174.28, is observable in chromatography paper.

In the chemical examination, chromatography could be a research facility strategy for the partition of a blend into its components.

The blend is broken down into a liquid dissolvable (gas or fluid) called the versatile stage, which carries it through a framework (a column, a capillary tube, a plate, or a sheet) on which a fabric called the stationary stage is settled.

Since the distinctive constituents of the blend tend to have diverse affinities for the stationary stage and are held for distinctive lengths of time depending on their intuition with their surface destinations causing them to partition.

The partition is based on the differential dividing between the portable and the stationary stages. Unpretentious contrasts in a compound's parcel coefficient result in differential maintenance on the stationary stage and hence influence the division.

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What is a cell GJFFVf xfhdfvcdd

Answers

Answer:

Yeah

Explanation:

In biology, the smallest unit that can live on its own and that makes up all living organisms and the tissues of the body’s a cell. A cell has three main parts: the cell membrane, the nucleus, and the cytoplasm. ... The nucleus is a structure inside the cell that contains the nucleolus and most of the cell's DNA.

Hope that helps

one liter of cacl2 solution contains 2.5 moles of cacl2. what is the molarity of th solution

Answers

The molarity of the CaCl₂ solution, which contains 2.5 moles of CaCl₂ in one liter, is 2.5 mol/L.

Molarity is a measure of the concentration of a solute in a solution, expressed as the number of moles of solute per liter of solution (mol/L). In this case, the given information states that one liter of the CaCl₂ solution contains 2.5 moles of CaCl₂.

To calculate the molarity, we divide the number of moles of solute (CaCl₂) by the volume of the solution in liters (1 L):

Molarity = Number of moles of solute / Volume of solution (in liters)

Molarity = 2.5 moles / 1 L

Molarity = 2.5 mol/L

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Which statement best explains the type of bond that will form between two elements from group 6A in the model?

A. The two elements will form a covalent bond because both elements will share a single electron in order to have full outer shells

B. The two elements will form a covalent bond because both elements will share a pair of electrons in order to have full outer shells.

C. The two elements will form an ionic bond because one of the elements will donate one electron to the other element in order to have full outer shells.

D. The two elements will form an ionic bind because one of the elements will donate two electrons to the other elements in order to have full outer shells.

*Will give brainliest*​

Which statement best explains the type of bond that will form between two elements from group 6A in the

Answers

Answer:

D is the answer

Like my answer

There are two types of chemical compound one is covalent compound and other is ionic compound, covalent compound formed by sharing of electron and ionic compound formed by complete transfer of electron. Therefore, the correct option is option D.

What is chemical Compound?

Chemical Compound is a combination of molecule, Molecule forms by combination of element and element forms by combination of atoms in fixed proportion.

An ionic compound is a metal and nonmetal combined compound.  Ionic compound are very hard. They have high melting and boiling point because of strong ion bond.

The two elements will form an ionic bind because one of the elements will donate two electrons to the other elements in order to have full outer shells.

Therefore, the correct option is option D.

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In reaction 1, if we had used 4 grams of NaOH instead of 2 grams, would the heat gained by the solution [#6] have been larger, smaller or the same? Explain Briefly!

Answers

If we had used 4 grams of NaOH instead of 2 grams, the heat gained by the solution would have been higher.

What is the enthalpy?

The term enthalpy has to do with the heat that could be emitted or evolved in a given reaction. We know that in a neutralization reaction, there is an evolution of heat and as such the reaction  vessel would feel warm at the end of the reaction.

Also, the enthalpy would depend on the amount of the limiting reactant. In this case, the limiting reactant would be the sodium hydroxide and as such the enthalpy would affected by a change in the amount of the sodium hydroxide.

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Nitrogen is inactive gas, why?​

Answers

because it doesn't react easily with other elements it requires high heat and other things

Both suspensions and colloids are heterogeneous mixtures. Define and characterize a suspension, listing similarities and differences to a colloid. Give several examples of suspensions.

Answers

Suspensions are similar to colloids in that they are both heterogenous mixtures and are not true solutions.

Suspensions differ from colloids in that the particle sizes are larger and settle when left to stand.

Some examples of suspensions are smoke and dust particles in the air.

What are suspensions and colloids?

Suspensions are a heterogeneous mixture of substances in which one substance is dispersed or distributed throughout a second phase. The particles of a suspension when left to stand will settles over time.

Some examples of suspension are dust particles dispersed in air, a gas.

Other suspensions may be solids dispersed in liquids such as in milk of magnesia.

Colloids or false solutions are a heterogeneous mixture of substances that are dispersed throughout a second medium and in which the particles do not settle when left to stand. The articles of a colloid can not be seen with the unaided eye.

Examples of colloids are gels, emulsions, and sols.

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What are electromagnetic waves?
I need answer pls

Answers

In physics, electromagnetic radiation refers to the waves of the electromagnetic field, propagating through space, carrying electromagnetic radiant energy. It includes radio waves, microwaves, infrared, light, ultraviolet, X-rays, and gamma rays

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Predict the immediate effect of the following changes on the observed cell voltage, E.
H2(g) + PbCl2(s) <–> Pb(s) + 2HCl (aq)
Delta H standard rxn > 0
Match
a) Dissolve NaOH into solution
b) Increase the temperature
c) Increase the amount of PbCl2
d) Dilute the solution by adding H2O
i) Increased E
ii) Decreased E
iii) No change in E

Answers

Dissolve NaOH into the Solution will - Increased E

Increase the temperature - Increased E

Increase the amount of \(PbCl_{2}\) - No change in E

Dilute the solution by adding \(H_{2}O\) - Increased E              

E= Voltage or the electromotive force generated in the cell

Dissolving NaOH will increase the conductivity and number of ions in the cell and Increases E .  Adding water also increases the presence of hydrogen and hydroxide ions in the solution and Increases E.

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How much is 1550 torr in atm?

(round to the hundredths place. The second decimal place)

Answers

1550 torr is equal to 2.04 atm, which is the standard unit of pressure and is used as an abbreviation for 'atmosphere'.

One atmosphere is the standard atmospheric pressure at sea level, and one atmosphere is used to indicate the pressure of gases or liquids in a system. In other words, a pressure of 1 atm refers to the pressure that the Earth's atmosphere exerts at sea level on the planet's surface.

To convert torr to atm, we can use the following conversion factor:

1 atm = 760 torr

Dividing both sides by 760, we get:

1 torr = 1/760 atm

Multiplying both sides by 1550, we get:

1550 torr = (1550/760) atm

1550 torr = 2.04 atm (rounded to the hundredths place)

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Long scratch marks that are left behind as evidence of glaciers are called
A. striations
B. cirques
C. fjords
D. roche moutonnees

Answers

Answer:

it is A striations

Explanation:

..........

Answer:

Striation

Explanation:

can someone please help me.

Answers

Answer:

Sure, with what?

Ps- Just to increase word count

Iodine monochloride (ICl) has a higher boiling point than bromine (Br2) partly because iodine monochloride is a(n)

Answers

Answer: polar molecule.

Explanation:

The boiling point is the temperature at which the vapor pressure of a liquid equals the external pressure surrounding the liquid. The boiling point is dependent on the type of forces present.

Iodine monochloride (ICl) is a polar molecule due to the difference in electronegativities of iodine and chlorine. Thus the molecules are bonded by strong dipole dipole forces. Thus a higher temperature is needed to generate enough vapor pressure.

Bromine \((Br_2)\) is a non polar molecule as there is no electronegativity difference between two bromine atoms. The molecules are bonded by weak vanderwaal forces and thus has low boiling point.

What are the two types of carbohydrates and food sources?.

Answers

Answer: simple and complex.

Explanation:These are also called simple sugars. They're found in refined sugars, like the white sugar you see in a sugar bowl. If you have a lollipop, you're eating simple carbs.

What does the word "kinetic" indicate about
particles in matter? W
A. large particles
B. moving particles
C. tiny particles
D. stationary particles

Answers

It’s B. The kinetic molecular theory states that matter is made up of constantly moving particles.

reactions and
ions.
Did your list include these ideas? Check all that
apply.
d the evolution of
actions are one
affected by

Chemical reactions involve the loss, gain, or
sharing of electrons, but do not involve
changes to the nucleus of an atom.
Nuclear reactions involve nges to the
nucleus and produce a new particle.
Chemical reactions involve low energy
changes.
Chemical reactions never involve a change in
the mass of atoms.
Nuclear reactions often involve a change in
the mass of an atom.
DONE
5 of 12

Answers

actions made by a verb. good luck bye

What kind of reaction is 21,02 → 2H,0 + 0,2
A. Single replacement
B. Double-replacement
C. Decomposition
D. Synthesis

Answers

Answer:

I think B

Explanation:

Double - replacement

It’s B I did this and I got it right not left

Solve you will get brainliest and23 points

Solve you will get brainliest and23 points

Answers

It’s the second one if you need more help tell me
Yes it is the second one

please answer all
1. Which of toluene or nitrobenzene is brominated faster during an electrophilic substitution reaction? Explain your answer and draw the reaction that occurs. Draw the reactions 2. During the measurem

Answers

Toluene is brominated faster during an electrophilic substitution reaction because it is more reactive towards the bromine water solution compared to nitrobenzene.

The reaction occurs as follows: Toluene reacts with bromine water in the presence of a catalyst such as iron (III) bromide to produce an intermediate, bromotoluene. Bromotoluene then reacts with bromine water to produce the final product, dibromotoluene. The electrophilic substitution reaction proceeds through the formation of a carbocation intermediate in the presence of a catalyst such as FeBr3.

The intermediate then undergoes attack by the electrophile, which in this case is bromine water, to produce the final product. Nitrobenzene, on the other hand, is less reactive towards electrophilic substitution reactions due to the presence of the nitro group which has an electron-withdrawing effect. This makes the carbocation intermediate less stable and hence less reactive toward the electrophile.

Therefore, nitrobenzene is brominated slower compared to toluene.

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The energy of the metal d orbitals _________ due to an increase in electrostatic repulsion?.

Answers

The energy of the metal d orbitals distributing a charge of −6 due to an increase in electrostatic repulsion.

What is crystal field theory?

The ability of transition metals to form complexes is a key property. A complex is made up of a core metal atom or ion surrounded by a number of ligands. Crystal field theory governs the interaction of these ligands with the core metal atom or ion.

Crystal field theory was developed in 1929 and treats the interaction between metal ion and ligand as a purely electrostatic phenomena, with the ligands seen as point charges in the region of the central atom's atomic orbitals. Crystal field theory was developed and extended to account for the partly covalent character of bonding between ligands and metal atoms, primarily through the application of molecular orbital theory.

To accurately grasp the crystal field interactions in transition metal complexes, knowledge of the geometrical or spatial distribution of d orbitals is required. In a free gaseous metal ion, the d-orbitals are fivefold degenerate. If a spherically symmetric field of negative ligand filled charge is imposed on a central metal ion, the d-orbitals remain degenerate but the energy of the free ion changes.

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A 8.5L balloon contains 3.5 moles of oxygen gas. If 2.3 moles are released from the balloon what is the final volume of the balloon?

Answers

Answer:

12.9L

Explanation:

V1 = 8.5L

P1 = 3.5

P2 = 2.3

V2 = ?

P1 V1 = P2 V2 ( Boyle's law )

3.5×8.5 = 2.3×V2

Divide both sides by 2.3

3.5×8.5/2.3 = 2.3×V2/2.3

V2 = 29.5/2.3

=12.9L

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