Why is calcium chloride used to make cells competent.

Answers

Answer 1

Calcium chloride is used to make cells competent because it disrupts the cell membrane and makes it permeable to foreign DNA. The positively charged calcium ions neutralize the negative charges on the DNA molecules and reduce the repulsion between them, which allows the DNA to enter the cell.

Transformation is a process by which cells take up DNA from the environment and incorporate it into their genome. To make this possible, the cell membrane needs to be made permeable to foreign DNA so that it can enter the cell. This process is known as making cells competent. Calcium chloride is used to make cells competent because it can disrupt the cell membrane and make it permeable to foreign DNA.

Calcium chloride is a salt that dissociates in water to form positively charged calcium ions (Ca2+) and negatively charged chloride ions (Cl-). When calcium chloride is added to cells, it neutralizes the negatively charged surface of the cell membrane and reduces the repulsion between the DNA molecules and the cell membrane. This allows the DNA to enter the cell more easily.

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Related Questions

which of the factors could lead to a prolonged increase in heart rate, even in the absence of an external signal? select all that apply. an inability of cyclic amp to activate protein kinase a an inability to inactivate protein kinase a an inability of a g protein to hydrolyze gtp an inability of g protein coupled receptors to bind adrenaline an inability to convert atp into cyclic amp

Answers

A prolonged increase in heart rate without an external signal could result from a malfunction in the signal transduction pathway involved in regulating heart rate, which could result from a defect in factors such as cyclic AMP, PKA, G proteins, or ATP conversion. Options A and B both are correct.

A prolonged increase in heart rate without an external signal could result from a malfunction in the signal transduction pathway involved in regulating heart rate. The regulation of heart rate is mediated by the sympathetic and parasympathetic nervous systems, which use signaling molecules like adrenaline and acetylcholine to modulate the activity of the heart. These signals bind to specific receptors on the surface of heart cells, which then activate intracellular signaling pathways to modulate heart rate.

Factors that could lead to a prolonged increase in heart rate include the inability of cyclic AMP to activate protein kinase A (PKA) or the inability to inactivate PKA. Cyclic AMP is a second messenger that is involved in the regulation of heart rate by activating PKA, which then modulates the activity of ion channels that control heart rate.

The inability to hydrolyze GTP by a G protein or the inability of G protein-coupled receptors to bind adrenaline could also lead to a prolonged increase in heart rate. G proteins are signaling molecules that are involved in the activation of intracellular signaling pathways in response to external signals, including adrenaline. If these proteins malfunction, it could result in a prolonged increase in heart rate.

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Complete question:

Which of the following factors could lead to a prolonged increase in heart rate, even in the absence of an external signal? Select all that apply.

A. Inability of cyclic AMP to activate protein kinase A

B. Inability to inactivate protein kinase A

C. Inability of a G protein to hydrolyze GTP

D. Inability of G protein-coupled receptors to bind adrenaline

E. Inability to convert ATP into cyclic AMP

To most humans, the chemical PTC tastes very bitter. The ability to taste it is a dominant trait. People who can’t taste PTC exhibit the recessive trait. Which genotype represents people who can’t taste the chemical?

Answers

The ability to taste the chemical PTC (phenylthiocarbamide) is determined by a single gene, TAS2R38, on chromosome 7. This gene codes for a taste receptor on the tongue that is sensitive to PTC.

The dominant allele (T) produces a functional receptor that can detect the bitter taste of PTC, while the recessive allele (t) produces a non-functional receptor that cannot detect the taste of PTC.

Therefore, individuals who can't taste the chemical PTC exhibit the recessive trait and have a homozygous recessive genotype (tt), as they have two copies of the non-functional allele. In contrast, individuals who can taste PTC can have either a homozygous dominant genotype (TT) or a heterozygous genotype (Tt).

This trait has been studied extensively in genetics research due to its simple inheritance pattern and high frequency of variation in human populations. It is estimated that approximately 25-30% of people worldwide are unable to taste PTC due to their homozygous recessive genotype.

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describe amendments 3 in 3 sentences

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A constitutional amendment to the documents of a government, organization, other type of entity. A constitution is often amended by inserting the necessary wording straight into the relevant parts.

What does amendment mean?

The provisions of a contract or other instrument may be amended or supplemented. An amendment is a new addition or change that largely maintains the integrity of the original document. The most important and frequent basis for constitutional amendments is the limiting of the charter of fundamental rights.

What is an example of a amendment?

A change, addition, or rephrasing of something with the goal of improvement is what is meant by the definition of an amendment. The amendments adopted to the United States Constitution are one example.

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What was the Treaty of Versailles clause?

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(I)The Rhineland's German portion was to be demilitarised, and the Allied Armies took control of this productive region.

(ii)Germany was required to pay the Allies 6 billion in war reparations for losses and damages incurred during the conflict.

The Treaty of Versailles was formally signed on June 28, 1919, bring an end to World War One. According to the conditions of the treaty, Germany was required to pay compensation, disarm, relinquish territory, and give it up all of its foreign possessions.The Rhineland's German portion was to be demilitarised, and the Allied Armies took control of this productive region. The Rhineland was taken by the newly formed Nations League, which also demilitarised it.Germany was required to pay the Allies 6 billion in war reparations for losses and damages incurred during the conflict. The number of soldiers in Germany's army was lowered to 100,000, and military conscription became forbidden. Its fleet of large ships was reduced, and the majority of its arsenal was taken. It is generally agreed that the Treaty of Versailles was really a failure because it subjected Germany to severe punishments and unreasonable demands, such as significant reparations payments & demilitarisation.

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(For this question, yellow is dominant and blue is recessive). What will the plants with YY and Yy look like? PLEASE HELP ASAP!!!
A. Cannot be determined
B. They will be different
C. Both the same

Answers

Answer:

C

Explanation:

Using resonance, select the most acidic protons in the hexane-2,4-dione molecule. The most acidic proton is the one that, upon removal, will yield the most stable conjugate base.

Answers

The correct option is B : The most acidic Protons are 4 or 5 . The four types of hydrogen with numbering are shown in the given figure. In order that a proton is acidic the conjugate base formed by removal of protons must be stable.

In case of given Hexane-2,4-dione molecule , the stability will be due to resonance. If we abstract a proton from the third carbon (between the two carbonyl carbons) it will result in formation of a carbanion, which is stabilized by resonance. The near by oxygen will give stability to the conjugate base by -I effect (negative inductive effect). So protons 4 or 5 gives most stable carbanion hence they are the most acidic protons .

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Full Question ;

Using resonance, select the most acidic protons in the hexane-2,4-dione molecule. The most acidic proton is the one that, upon removal, will yield the most stable conjugate base.

Check all that apply.

[Hexane-2,4-dione molecule with protons numbered as follows according to IUPAC nomenclature: protons on C1 are numbered 1 through 3, protons on C3 are numbered 4 and 5, protons on C5 are numbered 6 and 7, protons on C6 are numbered 8 through 10.]

Check all that apply.

a. Protons 1, 2, or 3

b. Protons 4 or 5

c. Protons 6 or 7

d. Protons 8, 9, or 10

true or false?two-thirds of our rainfall is returned to the air by evaporation and respiration

Answers

About 90% of the water in the atmosphere is produced from evaporation crom water bodys, while the remaining 10% comes from transpiration. These are the form of return of the water to the atmosphere in the water cycle. The rainfall is related to the process of precipitation of the water cycle, being know a the return of water to the Earth.

Therefore, for the information about the water cycle and how it work, the alternative is false because according to the known about the water cycle, rainfall is not direct linked to the return of water to the air, but to the return of water to the Earth.

Bradley improved his hitting after taking batting practice. A. Bradley improved his hitting B. after improvement C. taking batting practice D. practice with Bradley

Answers

A because the others didn't make no sense

Which of the following is an example of an ecosystem?

1. A herd of bison.
2. All the deer, rabbits, and flowers in McLean County.
3. A pride of lions, a herd of water buffalo, the grasses on the Savanna, and the drought
affecting them all.

Answers

A pride of lions, a herd of water buffalo, the grasses on the Savanna, and the drought affecting them all is an example of an ecosystem.

What is ecosystem?Ecosystem, the collection of living things, their physical surroundings, and all of their interactions within a certain spatial context.An ecosystem can be divided into its biotic constituents, which include all of its living members, and its abiotic constituents, which include minerals, the environment, soil, water, sunshine, and all other nonliving elements. The movement of energy through the ecosystem and the cycling of nutrients within the ecosystem are the two main forces that bind these components together. Ecosystems come in a variety of sizes; some can fit inside a single water droplet, while others can cover entire landscapes and countries.Radiant energy from the Sun is the primary source of energy in practically all ecosystems.

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Networks of neurons in the _____ lobe are involved in processing emotions and making plans. Question options: a) occipital b) temporal c) parietal d) frontal

Answers

Networks of neurons in the frontal lobe of the brain are primarily responsible for processing emotions and making plans.

Here correct option is D.

The frontal lobe plays a crucial role in higher cognitive functions, including executive functions such as decision-making, problem-solving, and goal-oriented behavior. It is involved in emotional regulation, social behavior, and personality expression.

Additionally, the frontal lobe houses the prefrontal cortex, which is associated with complex thinking, personality traits, and self-control.

Damage to the frontal lobe can lead to changes in emotion regulation, impaired decision-making abilities, and difficulties in planning and executing tasks.

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you are a marathon runner and need energy for tomorrow's race. how would eating pasta (and pie) help your body produce the energy it needs? Be sure to describe what will happen when you are running the race ( and breathing hard).

Answers

The pasta(and the pie) are full of carbs and sugar and will help your energy levels go up

Which of the following was not a cause of 34 countries being affected by food emergencies at the end of the 20th
century?
A) varying weather patterns
B) food distribution issues
C) inadequate trade agreements
D) decreased aid from other countries

Answers

Answer: I think it’s c

Explanation:

Answer:

D.) Decreased aid from other countries

Explanation:

Got it right on Edge

on average, prokaryotic cells are smaller than eukaryotic cells. what are some advantages to small cell size? what are some advantages to large cell size?

Answers

It allows an easy transport of substances across the plasma membrane. Small cells have a higher surface area to volume ratio, which provide them a greater surface area for the exchange of nutrients and waste materials by spending relatively lesser energy.

the mucosa of the ureter is formed from ______ epithelium.

Answers

The mucosa of the ureter is formed from transitional epithelium.

Transitional epithelium is a specialized type of epithelial tissue that lines organs that undergo significant expansion and contraction, such as the urinary bladder and ureters. It is characterized by cells that can stretch and flatten to accommodate changes in the organ's volume without rupturing the tissue. The cells of transitional epithelium also have a unique ability to resist the diffusion of urine and prevent the reabsorption of harmful substances from the urinary tract. The mucosa of the ureter, which is responsible for the secretion of mucus and the absorption of water and electrolytes, is therefore formed from transitional epithelium.

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Question 6(Multiple Choice Worth 3 points)
(04.02 MC)
Drought is the lack of soil moisture over a period of time. This lack of moisture can be due to which of the following?
O Reduced precipitation

Increased evaporation and/or

transpiration

Higher temperature

All of the above

Answers

Answer:

all of the above

...........

all of the above
…………

What sets adolescents' thinking apart from that of younger children?
A) ability to remember thoughts they have had previously
B) ability to develop a sense of right and wrong
C) ability to think beyond the concrete situation to what might or could be
D) ability to understand the concept of time

Answers

C) ability to think beyond the concrete situation to what might or could be sets adolescents' thinking apart from that of younger children. While younger children tend to think in concrete terms and focus on the present, adolescents are able to think abstractly and consider possibilities and potential outcomes in the future.

This cognitive shift is a key component of their development and allows them to engage in more complex problem-solving and decision-making. The ability to think beyond the concrete situation to what might or could be is known as abstract thinking. It involves the ability to understand and analyze concepts and ideas that are not directly tied to concrete, tangible objects or experiences. Abstract thinking allows individuals to consider hypothetical situations, solve problems using reasoning and logic, and engage in creative thinking.

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single-unit recording data shows us: 1 pointgrading comment:choice a. 1 of 4:how intense a single action potential waschoice b. 2 of 4:how frequently a neuron firedchoice c. 3 of 4:how many single neurons are in a networkchoice d. 4 of 4:all of the above

Answers

The correct option is D, single-unit recording data shows us how intense a single action potential was choice how frequently a neuron fired choice how many single neurons are in a network choice.

A neuron is a specialized cell that is the fundamental building block of the nervous system. It plays a vital role in transmitting and processing information in the form of electrical and chemical signals. Neurons are complex, consisting of three main parts: the cell body (soma), dendrites, and an axon.

The cell body contains the nucleus and other cellular components necessary for the neuron's functioning. Dendrites are branch-like structures that receive incoming signals from other neurons or sensory receptors. The axon is a long, slender projection that carries electrical impulses away from the cell body to transmit signals to other neurons or target cells. Neurons communicate with each other through synapses, which are junctions where signals are transmitted chemically.

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Which of the following statements is/are correct concerning biodiversity?

Biodiversity has a spiritual value


The conservation of biodiversity is crucial to protect our health


Food biodiversity is the central component of quality diet and food safety


Global anthropogenic change is the cause of the unprecedented loss of biodiversity in the planet


Biodiversity refers to diversity of life in all its forms, from the molecular level to the ecosystem level

Answers

The statement which are correct regarding biodiversity are the conservation of biodiversity is crucial to protect our health and Global anthropogenic change is the cause of the unprecedented loss of biodiversity in the planet.Thus, option B and D are correct.

Who contributes to maintain the balance of nature?

Every species on the Earth contributes significantly to the nature's intricate balancing act. Forests are the fragmented as the result of the natural disturbances just like fires or changes in land use like building roads.

As the result of these habitat alterations, biodiversity has significantly impacted since limited habitat fragments can only maintain weak populations that are more susceptible to extinction.

Therefore, The statement which are correct regarding biodiversity are the conservation of biodiversity is crucial to protect our health and Global anthropogenic change is the cause of the unprecedented loss of biodiversity in the planet.Thus, option B and D are correct.

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where is the crater from the asteroid that killed the dinosaurs

Answers

Answer:

it's Chicxulub crater

Explanation:

good luck hope it helps

Why is the cell membrane not as noticeable in a plant cell than in an animal cell?

Answers

Plant cells have a cell wall, plastids, such as chloroplasts which contain the green photosynthetic pigment chlorophyll in green plants, a vacuole which is an organelle(cellular subunit) that contains water allowing the plant to maintain turgidity(turgor pressure allows plants to have rigid structure when the water pushes against the vacoule containing water.) An example of turgidity/turgor pressure is soaking lettuce leaves in water to maintain its crispness. Plants have stomata in their cells, which allow leaves to let water enter and exit the cell. If you have ever felt a mist coming from a tree you are sitting under on a hot summer day, then you have felt water coming from the tree's leaves. This phenomenon is called transpiration.

what is natural selection

Answers

Answer:

the process whereby organisms better adapted to their environment tend to survive and produce more offspring. The theory of its action was first fully expounded by Charles Darwin and is now believed to be the main process that brings about evolution.

Explanation:

what is polygenetics ?​

Answers

Explanation:

is a member of a group of non epics tactic genes that interact additively to influence a phenotypic trait

Answer (three definitions, where two are biology and one is geology):

1) (biology) Of or pertaining to polygenesis; polyphyletic.

2) relating to or determined by polygenes.

If you don’t know what a polygene is: A polygene or multiple gene inheritance is a member of a group of non-epistatic genes that interact additively to influence a phenotypic trait.

3) (geology) One which is composite, or consists of two or more monogenetic ranges, each having had its own history of development.

Hope this helps! :)

Factors that affect the entropy of a system. List all the factors that affect the amount of entropy of a system and describe how each of them does so.

Answers

Entropy is a metric for a system's disorder or randomness. The entropy of a system can be influenced by any factor that alters the arrangement and mobility of its constituent particles.

The entropy of a system can be impacted by a number of things, such as:

1. Temperature: A system's temperature has an impact on its entropy since rising temperatures induce particles to move more quickly and disorderly.

2. Pressure: A decrease in volume caused by an increase in pressure can result in an increase in order and a decrease in entropy.

3. Volume: A system's volume may have an impact on its entropy. As the volume grows, there is more room for particles to move, which causes the disorder.

4. Particle count: A system's entropy can be impacted by its particle count because more particles increase the variety of possible configurations.

5. Molecular complexity: Because more complex molecules have more potential arrangements, the complexity of the molecules within a system can alter its entropy.

6. Mixing: Because additional alternative arrangements are created when different chemicals are mixed together, this process can make a system more entropic.

7. Chemical reactions: By generating new compounds with various configurations, chemical reactions can change the entropy of a system.

8. Phase changes: By altering the arrangement and mobility of particles, phase shifts, such as melting or boiling, can have an impact on the entropy of a system.

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The organization of similar organisms into groups helps scientists understand how living things are related. It
also allows scientists to communicate about all forms of life. Scientists organize the living world using a process
called taxonomy, which is the science of classifying organisms based on shared structures, functions, and
relationships to other organisms. Scientists use a branching system of classification, and over the years, due to
new discoveries, the branching system has changed and grown.
Recently, scientists broadened the branching system of classification. Currently, the broadest group is the
A)
class
B)
domain
kingdom
D)
taxon
taxon

Answers

Answer:

Explanation:

THE ANSWER IS DOMAIN

Question 1. Construct a crossword puzzle of your own using the keywords. (Chapter - 18 Pollution of Air and Water)​

Answers

can you send keywords?

Tumors in an endocrine gland cause unregulated release of the hormone or hormones produced by that gland. It there were a tumor in the endocrine part of the hypothalamus that acts on the anterior pituitary, which of the following would result?

A) Hormone from the third endocrine gland would increase but hormone from the hypothalamus and anterior pituitary would decrease.
B) Only hormones from the anterior pituitary would increase.
C) All hormones in the cascade would increase.
D) None of the hormone levels would be changed because of negative feedback.
E) Hormone from the hypothalamus would decrease, but hormone from the anterior pituitary and third endocrine gland would increase.

Answers

Answer: E)

Explanation:

When a tumor develops in an endocrine gland, it can cause unregulated release of the hormone or hormones produced by that gland. If there were a tumor in the endocrine part of the hypothalamus that acts on the anterior pituitary, the answer would be E) Hormone from the hypothalamus would decrease, but hormone from the anterior pituitary and third endocrine gland would increase.

The hypothalamus produces and releases hormones that act on the anterior pituitary to regulate the release of hormones from other endocrine glands. If a tumor were to develop in the hypothalamus, it could disrupt the normal feedback loop and cause unregulated release of hormones. In this case, the hormone from the hypothalamus would decrease because the tumor would be disrupting its release. However, the hormone from the anterior pituitary and third endocrine gland would increase due to the disruption of the normal feedback loop.

So, in summary, a tumor in the endocrine part of the hypothalamus that acts on the anterior pituitary would result in a decrease in hormone from the hypothalamus, but an increase in hormone from the anterior pituitary and third endocrine gland.

Q1. what light-dependent reactions of photosynthesis occur here?

Q2. what light-independent reactions of photosynthesis occur here?

Answers

Answer:

The light-dependent reactions of photosynthesis take place within the thylakoids. These reactions occur when the pigment chlorophyll, located within the thylakoid membranes, captures energy from the sun (photons) to initiate the breakdown of water molecules.The light-independent reactions of photosynthesis take place within the stroma. It contains enzymes that work with ATP and NADPH to “fix” carbon from carbon dioxide into molecules that can be used to build glucose. The chloroplast's own genetic material (separate from that of the cell) is also stored in the stroma.

Cellular respiration is the cellular process which transfers
chemical energy from the sugar molecule glucose into what
other molecule?
a ADP
b ATP
C NADPH
d FADHA

Answers

B. ATP

hope this helps !

the sarcoplasm of muscle cells contain cylindrical organelles called , which make up 50-80% of its volume.

Answers

The barrel-shaped organelles that makeup 50-80% of the volume of sarcoplasm in muscle cells are called myofibrils. Myofibrils are technical organelles found in muscle cells that control how muscles contract and relax.

They are made out of rehashing units called sarcomeres, which are the fundamental contractile units of muscle.

There are two kinds of protein filaments in each sarcomere: actin filaments which are thin and myosin filaments which are thick. During muscle contraction, these filaments slide past one another, shortening the sarcomere and causing the muscle cell to contract as a whole.

Under a microscope, striated skeletal muscle is created by a high concentration of myofibrils in muscle cells.

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Proteins do not pass through cell membranes because.

Answers

Answer:

Proteins are generally way too big to be able to fit through a cell membrane.

Explanation:

They cannot compress their molecules to pass through.

Other Questions
The battle, sir, is not to the strong alone; it is to the vigilant, the active, the brave. Besides, sir, we have no election. If we were base enough to desire it, it is now too late to retire from the contest. There is no retreat but in submission and slavery! Our chains are forged! Their clanking may be heard on the plains of Boston! The war is inevitableand let it come! I repeat it, sir, let it come.What does Henrys use of exclamatory remarks and repetition reveal about the speaker?A. They emphasize that he is willing to compromise on a solution.B. They explain why he feels that war against the British is necessary. C. They reveal that he has gathered a number of facts to support his argument.D. They show his strong emotional support for going to war against the British. The cost of 3 paninis and 2 drinks is 12 The cost of 7 paninis and 2 drinks is 20. how much does panini and drinks cost for an electromagnetic wave the direction of the vector e x b gives a=5b=12c=10d=21. 2b-a2. d(ab-c)3. 3 + b/d4. 4a/b+4d5. b-c+d ________ is valuable because it can be used as a nonbureaucratic conduit for information flows within a multinational enterprise.A) A liaison networkB) A matrix structureC) An organizational structureD) A knowledge network a tendency to notice and remember instances in which our hunches about future disaster are subsequently followed by harmful events contributes to a sports writer studies the batting average, walk to strikeout ratio, and home runs hit for a certain baseball player for five weeks for an article. what might prevent a species in a mutualistic relationship from reaping the benefits from a partner without providing a benefit in return? PLEASE HELP!! WILL GIVE BRAINLIEST!!! TYSM!!! Complete each sentence with the correct form of the verb using the present tense. En la escuela yo __1__ (estudiar) matematicas, ciencia, historia, y geografia. Y tu, __2__ (estudiar) tu las mismas en tu escuela? Naturalmente yo __3__ (hablar) espanol y mis amigos y yo tambin __4__ (practicar) ingls en la escuela. Generalmente yo __5__ (sacar) buenas notas en las pruebas porque yo __6__ (escuchar) a todas las explicaciones de los profesores. Mis dos amigos, Juan Ramon y Diego __7__ (trabajar) mucho tambin. Which one of the following is one of the Theodore Levitt assumptions supporting a pure global strategy?a. Consumers are willing to pay more for specific product features.b. Customer needs and interests are becoming more dissimilar.c. MNCs can successfully compete globally by aggressively pricing products at the sacrifice of product features.d. If the world markets are treated as heterogeneous, substantial economies of scale are easily achieved. You and your friend are standing back-to-back. Your friend runs 16 feet forward and then 12 feet right. At the same time, you run 12 feet forward and then 9 feet right. You stop and throw a baseball to your friend, who catches it. How far did you throw the baseball? Solve the application problem. Round to the nearest percent.Last month, Jones Used Car Lot sold $150,000.00 worth of cars. This month their car sales reached $450,000.00. What was their percent increase in car sales? Anne Kates house is 20c. She had just made a fresh cup of tea (100c). Five minutes after she made her mad scientist nephew came in,stuck a thermometer in the cup and announced that the tea was now only 70c Write the possible equation with asymptotes Find the value of angle c. Give your answer indegrees ().58150126C=?? The two key complementary components of the Social Security Act were: hey guys I need this fast. and please show step by step because I'm willing to give brainly A two-year, $1,000 (i.e., face value) bond that pays an annual coupon of 8 percent and trades at a yield of 8 percent.Calculate Macaulay duration.Group of answer choices1.9259 years1.9247 years2 years1.7833 years1.9106 years HELPPPP meeeee!!! correct answers only please What can or did you infer about the characters based on their names in The Phantom Tollbooth? Provide specific examp Assume a therapist completes this rating scale for a patient today and then completes this rating scale again for the same patient one week later. If the therapist's ratings were the same both times (e.g., 0 or "Lowest Concern"), this would be an example of high _________.a. convergent validityb. predictive validityc. inter-rater reliabilityd. internal consistencye. test-retest reliability