why do you need to add two primers to each pcr reaction?

Answers

Answer 1

In PCR (Polymerase Chain Reaction), two primers are added to each reaction because they serve as the starting point for DNA amplification.

Primers are short pieces of DNA that are complementary to the target DNA sequence, and they bind to the DNA strands to be amplified, allowing the DNA polymerase enzyme to synthesize new strands of DNA.

By designing primers that are specific to the target DNA sequence, PCR can selectively amplify only the desired DNA fragment. Using two primers in each reaction ensures that both strands of the double-stranded DNA template are amplified, leading to a more efficient and accurate amplification of the target DNA sequence.

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Related Questions

When ethical problems arise, one side is clearly in the right while the other is in the wrong.


A. True
B. False

Answers

Answer:a true

Explanation:

Answer:

False

Explanation:

Ethics are the standard of what is right and wrong, and they are based on our values. Being ethical requires making a moral judgment, and that’s not always easy. Ethical behavior takes courage and has to be practiced. Public officials feel added pressures. The ethical choices we make often occur in the public arena, often under the media’s lens.

To become a better dancer it is best to dance with people of varying abilities, and it also gives everyone a chance to dance.

True
False

Answers

Answer:

The answer is true.

Explanation:

Many people may dance differently depending on their physical appearances. Athletic people may dance faster, or better. While calm people may dance slower, or more elegantly.

Ladonna is overweight and loves fast food. Which is the best diet for Ladonna to adopt?
A.
a diet high in protein and low in fat and sugar
B.
a diet high in calcium and low in fiber
C.
a diet high in fiber and low in fat and sugar

Answers

Based on the information provided, the best diet for Ladonna to adopt would be option C, a diet high in fiber and low in fat and sugar.

A diet high in fiber and low in fat and sugar (option C) can be a good choice for Ladonna because it can help her feel full and satisfied, making it easier to resist fast food cravings. High-fiber foods, such as fruits, vegetables, and whole grains, can also aid in digestion and help regulate blood sugar levels.

A diet high in protein and low in fat and sugar (option A) may be helpful for weight loss, but it does not necessarily address Ladonna's love for fast food, which is often high in fat and sugar. Additionally, a diet too high in protein can strain the kidneys, especially if Ladonna has pre-existing kidney issues.


A diet high in calcium and low in fiber (option B) may also not be the best choice for Ladonna. While calcium is important for bone health, it does not really aid in weight loss, and a diet too low in fiber can lead to constipation and other digestive issues.

What is ''being healthy''?

Answers

Being healthy means being able to continue your normal life without bad conditions.

A 50-year-old man with diabetes has an altered mental status and is unable to tell you when he last ate or took his insulin. Your glucometer keeps malfunctioning and you are unable to determine his blood glucose level. What clinical signs would MOST likely lead you to the correct diagnosis?

Answers

There are several possible reasons for the patient's altered mental status, including hypoglycemia, hyperglycemia, and other metabolic disturbances, as well as central nervous system (CNS) disorders.

Hypoglycemia is the most likely diagnosis, particularly in diabetic patients who are receiving insulin therapy. Hypoglycemia, a condition caused by low blood sugar, is one of the most common metabolic emergencies seen in both diabetic and non-diabetic patients.The most common symptoms of hypoglycemia include confusion, irritability, diaphoresis, headache, and blurred vision. Because the patient in this situation is unable to communicate, these symptoms may not be present. Signs of hypoglycemia are characterized by the body's compensatory mechanisms, which are aimed at restoring blood glucose levels to normal. This would include tachycardia, tremors, pallor, and diaphoresis. Hypotension and tachypnea can occur as the hypoglycemia worsens.In conclusion, Hypoglycemia, which is characterized by signs and symptoms such as tachycardia, tremors, pallor, diaphoresis, hypotension, and tachypnea, is the most common cause of altered mental status in diabetic patients receiving insulin therapy.

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Name and explain the socio-economic implications of
poor health care system in a nation.

Answers

A poor healthcare system in a nation can have significant socio-economic implications. First, it can lead to a reduction in the productivity of the workforce. When people are not healthy, they cannot perform at their best, which can result in lower levels of productivity in the workplace.


Second, a poor healthcare system can have a significant impact on the economy of a nation. The cost of treating diseases and illnesses can be high, and in many cases, people who cannot afford to pay for healthcare must seek alternative treatment options or go without treatment. This can lead to a cycle of poverty, where people are unable to access healthcare services and, as a result, their health deteriorates further.Third, a poor healthcare system can also have a significant impact on the education sector. When children are not healthy, they are likely to miss school, which can result in lower levels of educational attainment. This, in turn, can have long-term implications for the economic and social development of a nation.



In conclusion, a poor healthcare system can have significant socio-economic implications for a nation. It can result in lower levels of productivity, a cycle of poverty, and lower levels of educational attainment. Therefore, it is essential for governments to invest in healthcare systems to ensure that all individuals have access to proper healthcare facilities.

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Ritalin and the pain medication Demerol have a high risk of dependence but may be used for medicinal purposes under a doctor's supervision. These substances are ___ of the CSA list.

1.) in schedule II
2.) in schedule III
3.) in schedule I
4.) not on the list

Answers

Ritalin and the pain medication Demerol have a high risk of dependence but may be used for medicinal purposes under a doctor's supervision. These substances are:

1.) in schedule II

2.) in schedule III

What is the medication about?

According to the Controlled Substances Act (CSA) classification in the US, Ritalin and Demerol are regarded as controlled substances.

Ritalin is a medication that has a stimulating effect and is commonly prescribed for the treatment of Attention Deficit Hyperactivity Disorder (ADHD). On the other hand, Demerol is a drug that is designed to relieve pain ranging from moderate to intense.

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Andy just lost his job. His family is now relocating to live with extended family. Which is an internal pressure that will most likely create conflict?

Answers

Andy will now be forced to adjust to a new environment which involves having to meet new people which can be pressuring

Answer:

D. disagreeing about which school kids should attend

Explanation:

I took the test, if you know what I mean ;)

Carol, age 68, was brought to the emergency department by her husband when she was having difficulty breathing. She had also developed a cough and fever, and her husband noticed that her symptoms seemed to occur suddenly.Based on the information available, you assess Carol for pneumonia.
Which of the following assessment results would indicate Carol may have pneumonia? Select all that apply.

Answers

The other results which may indicate that Carol is suffering from pneumonia are Crackles, Whispering sounds clear and distinct and Increased tactile fremitus.

Pneumonia is a disease in which the inflammation in lungs causes difficulty in breathing due to which fever and headache may be seen in the person. Crackles is a symptom of pneumonia in which the alveoli are opening and closing against fluid rapidly. Whispered sounds and tactile fremitus are more distinct sounds from the lungs when pneumonia is actually detected in harsh condition. Pneumonia is caused due to Streptococcus pneumoniae bacteria. It is most common in children and old people because of the weak immune system.

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How can skilled players encourage those with less ability?

Answers

Answer:

Skilled players can encourage those will less ability by befriending them. This can lead to connects which makes that skilled player a mentor for the those with less ability.

Skilled player encourages with less ability players by building a friendly relationship and guiding them properly on their mistakes as well as weaknesses.

What do you mean by Skilled player?

Skilled players are those who have perfect in their sports and consist of very few or no weaknesses.

Skilled players have to coach the less-ability player and try to give more focusses on their mistakes and resolve them all with regular practice, hard work, and determination. All this is possible when both of these players have be friendly and have no ego in their behavior.

Therefore, Skilled player encourages with less ability players by building a friendly relationship and guiding them properly on their mistakes as well as weaknesses.

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Which kind of device helps people manage their health? digital cookbook wireless hot spot handheld video game digital blood glucose monitor

Answers

digital blood glucose monitor

Module 5: Family as Client: Public Health Clinic. Discuss the Somali Family Strengths and Nursing Interventions.

Answers

The Somali family is characterized by strong family bonds and a deep sense of community.

They value their cultural traditions, and have a strong sense of religious faith. These family strengths can be leveraged in nursing interventions to promote health and well-being.

One nursing intervention that can be used with Somali families is to involve family members in the care process. This can include involving them in decision-making, providing education about health conditions and treatments, and encouraging them to support one another during the recovery process.

Additionally, nurses can work to build trust with Somali families by respecting their cultural traditions and religious beliefs, and by communicating in a respectful and culturally sensitive manner.

Another important nursing intervention is to provide culturally appropriate care. This can include using interpreters to facilitate communication, and providing information about health care services that are available in the community.

Nurses can also work to ensure that Somali families have access to appropriate resources, such as healthy food and safe housing.

Overall, it is important for nurses to recognize the strengths of Somali families and to use these strengths to support and promote health and well-being. By using culturally appropriate interventions, and by building strong relationships with Somali families, nurses can help to improve health outcomes for this population.

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Treatment for burns depends on the ____________ of the burn.

Answers

Answer:

degree

Explanation:

1st degree is like a boil and second is like your skin peeling and 3rd is GO TO THE HOSPITAL

Answer:

I would say significance.

The backbone of a phospholipid is which of the following?
A) Glycerol.
B) Cholesterol.
C) Fatty acid chain.
D) Triacylglycerol.
E) Glucose.

Answers

The backbone of a phospholipid is (a) glycerol.

Glycerol is the backbone of a phospholipid. The polar hydrophilic head, which contains a phosphate group, is connected to the glycerol backbone.

On the other hand, the hydrophobic tail is made up of two fatty acid chains that are connected to the other two carbons of the glycerol backbone.

The phospholipids are made up of a polar head and non-polar tail. They have a head and a tail structure, with the head being polar and the tail being nonpolar. The tail is made up of fatty acids, while the head is made up of a polar substance.

Phospholipids are amphiphilic, which means they have both hydrophilic and hydrophobic characteristics. They have a hydrophilic head and a hydrophobic tail due to their molecular structure. The tail is hydrophobic because it is made up of fatty acids, which are nonpolar and therefore do not dissolve in water. The head, on the other hand, is hydrophilic because it contains a phosphate group and is polar, which means it dissolves in water.

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the best way to deal with stress or a stressful situation is to​

Answers

Even if you can't change your circumstance, you can still reduce the stress you experience as you go about your everyday existence. Certain tasks can foster resilience, make you feel less stressed generally, and help you be less reactive to stresses when they appear. Take Care of Yourself

When you are fatigued, famished, and exhausted, everything feels more stressful. It also implies that you have lesser coping tools available to you. This means you're more apt to react to worry than respond to it. Alternatively, you could let things escalate until they become even more overpowering.

Focus on taking care of your body, and you will be able to manage anger and tension better.Do activities that make you feel good and cared for, such as having a massage, perusing a novel, or treating yourself to a relaxing spa day at home.

Exercise

Exercise is one of those great stress relievers that can help you burn off steam and develop fortitude. Even better, consistent exercise can help you become less receptive to worry. Because of this, as well as the clear health advantages of exercise, this is a tension reliever that should be incorporated into your plan whenever feasible. Meditation can help you stay focused in the face of tension and recover a sense of calm when you are feeling off-kilter. Many meditation methods are effective, so attempt a few and stick with them.preferred method that feels natural. Mindset of Positivity

One thing you can do to make everything in your life feel simpler is to keep a positive mindset. A positive mindset also helps you get along with others better (which can lead to more social support and less conflict) and can help you feel happy even when things around you aren't going so well.

Think of two Pharma professions that helped cause the opioid epidemic: the marketers and the salespeople themselves.Now imagine you're reporting on them.What were the main causes that allowed them to continue to market and sell as they did? How were convinced to do these jobs, despite the possible results? How did the bosses and other leaders at Pharma motivate them?

Answers

The main causes that allowed marketers and salespeople in the pharmaceutical industry to continue marketing and selling opioids despite the potential consequences were aggressive marketing strategies, financial incentives, company culture, and the lack of regulatory oversight.

Pharmaceutical companies engaged in deceptive marketing, downplaying the addictive potential of opioids and promoting them as safe and effective for long-term pain management. Salespeople were enticed by high salaries, bonuses, and other incentives based on sales performance. Company leaders fostered a culture that prioritized sales targets over ethical considerations, while weak regulations and limited monitoring provided minimal consequences. Understanding these factors is crucial for preventing future crises and promoting responsible marketing practices in the pharmaceutical industry.

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Explain a current regulation regarding the existing initiative that has been identified and the context in which it was developed. Why is this regulation important?
Describe the regulatory level that the existing initiative is being addressed at. Consider the potential for multiple levels of regulation.

Answers

A current regulation regarding the existing initiative that has been identified is important because it helps ensure that the initiative is implemented safely, effectively, and in accordance with established standards and guidelines .

For instance, some initiatives may be regulated at the federal level, while others may be regulated at the state or local level .There is a potential for multiple levels of regulation because different jurisdictions may have different standards, guidelines, and regulations governing the same issue or initiative. Therefore, it is important to consider the regulatory level at which the initiative is being addressed and to ensure that it complies with all applicable regulations.

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Which is an acceptable method of selecting an appropriately sized oropharyngeal airway?.

Answers

The acceptable method of selecting an appropriately sized oropharyngeal airway is by measuring from the tip of the patient's earlobe to the tip of the patient's nose.

What is oropharyngeal airway?

The oropharyngeal airway is defined as the device used by a medical personnel to help maintain a patent airway for patients especially when unconscious.

In order to measure the actual size for each patient to know the exact oropharyngeal airway device to use, place the device at the tip of the patient's earlobe to the tip of the patient's nose.

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Samara i nine year old and ha tarted to truggle with getting ready for chool. She tell her mom that he hate the way that he look and doen't like any of her clothe. Her mom ha noticed Samara expreing concern not only about her appearance but alo about failing or not meeting expectation. Thi i likely becaue chool-aged children often experience iue with:

ympathy. Happine. Intelligence. Elf-eteem

Answers

This is perhaps because self-esteem problems are common among school-aged youngsters.

Our self-esteem can be determined by how we view and treat ourselves.

It is predicated on our self-perceptions, which may be challenging to alter. This is also referred to as confidence. Depending on how much you value yourself as a person, you might: Like and respect yourself.

What leads to low self-esteem?

Stress and difficult life events, like a significant sickness or a loss, can have a negative affect on one's self-esteem. Personality could be crucial as well. Some people just seem to think more pessimistically, while others have excessive self-confidence.

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high efficiency particulate air (hepa) filters are required in the exhaust ventilation for which laboratories?

Answers

HEPA filters are required in various types of laboratories to ensure the safety of the lab workers and to maintain the integrity of experiments. Laboratories dealing with hazardous materials, biological agents, and cleanrooms.

High-Efficiency Particulate Air (HEPA) filters are commonly used in various types of laboratories to ensure the safety of the lab workers and to maintain the integrity of the experiments. HEPA filters are designed to remove particulate matter from the air, including dust, pollen, mould, and bacteria. These filters are required in the exhaust ventilation of laboratories that deal with hazardous materials or biological agents.

Some of the laboratories where HEPA filters are required include chemical and pharmaceutical labs, microbiology labs, research labs, and cleanrooms. In chemical and pharmaceutical labs, HEPA filters are used to remove harmful fumes and chemicals from the air, while in microbiology labs, they are used to prevent the spread of airborne microorganisms that can cause infections. Research labs may also require HEPA filters to protect the integrity of experiments by preventing outside particles from contaminating samples.

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emergency restraints or seclusion may be implemented without a physician's order under which condition?

Answers

Emergency restraints or seclusion is a last resort measure that is used in emergency situations when a person poses a risk to themselves or others. This measure is only implemented when it is deemed necessary for the safety and well-being of the individual and those around them.

It is important to understand that it is not a decision that is taken lightly and only used when all other alternatives have been exhausted.

In some cases, emergency restraints or seclusion may be implemented without a physician's order. This is usually done in emergency situations where a person is in immediate danger and there is no time to wait for a physician's order. For example, if a person is experiencing a mental health crisis and is acting in a manner that could cause harm to themselves or others, emergency restraints or seclusion may be used to protect them and those around them.

It is important to note that emergency restraints or seclusion should only be used as a short-term solution and must be followed by a comprehensive evaluation of the individual's situation. This evaluation is performed by a team of mental health professionals who will determine the best course of action for the individual's well-being.

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A mnemonic device is a strategy used to improve memory.truefalse

Answers

Answer:A mnemonic device is a strategy used to improve memory. It is possible for a mix up to occur between fictitious and factual information. Creating a mental image wherein items to be remembered are linked together is an example of the link method.

how can you be safe at home ? mention any two ways​

Answers

Answer:

7 Safety Rules for Kids at Home Alone

Have an emergency plan. Inspect your home thoroughly for any safety risks. ...

Always check in. ...

Make the phone (almost) off-limits. ...

Don't leave the house. ...

Take the same way home. ...

Keep him busy. ...

Practice first aid.

Answer:

Don't neglect safety housekeeping. ...

Maintain adequate home security. ...

John overhears two people talking in the cafeteria. He learns that there is a lice outbreak in the hospital. This is an example of _____.


mass communication
mass communication

nonverbal communication
nonverbal communication

unfocused communication
unfocused communication

focused communication
focused communication

Answers

Mass communication is the answer I think

Circle the one that is not a mental health professional:

counselor

sociologist

psychologist

social worker

psychiatrist

Answers

Answer:

A social worker is not a mental health profession because it does not require knowledge or a degree in psychology to get this job.

Explanation:

Possible effects of this drug include: euphoria, slowed reaction time, relaxed inhibitions, increased
appetite, impaired memory (up to 3-4 weeks after its use) and shortened attention span.
А) РСР
B) ecstacy
C) heroin
D) marijuana

Answers

The answer your looking for is D) marijuana, but in my opinion this is not factual.

The __________ theory aspect of the biological perspective maintains that natural selection has favored certain traits over the course of human history, resulting in specific, current traits. a. twin study b. three-factor model c. person-centered d. evolutionary

Answers

Answer:

The answer is D. Evolutionary.

Explanation:

The evolutionary theory aspect of the biological perspective maintains that natural selection has favored certain traits over the course of human history, resulting in specific, current traits.

Can i use sweetened condensed milk instead of evaporated milk?

Answers

Yes you can it might be a little different tasting but yea you can

Which statements are true about breast cancer
females of any age (and some males) can get breast cancer.
90% percent of breast cancer cases occur in women who are over the age of 60
A mammogram test usually detects breast cancer.
Another way to check for breast cancer is to do a breast self-examination (BSE) each month.
women who do not have a family history of breast cancer disease will not get breast cancer.

Answers

Answer:

females of any age (and some males) can get breast cancer.

A mammogram test usually detects breast cancer.

Another way to check for breast cancer is to do a breast self-examination (BSE) each month.

Explanation:

A mammogram test typically detects breast cancer, as per research, which is a true statement about breast cancer. The correct option is C.

What exactly is a mammogram?

It is a radiographic image of the breast that allows benign or malignant tumors of various sizes to be identified.

For some women, the compression can make a mammogram painful, but for the majority, it is only pretty unpleasant, and the sensation lasts only a few seconds.

Nonetheless, some women may avoid regular mammograms because they are concerned about the discomfort.

Such imaging aids doctors in the early detection of breast cancer, a type of cancer of the reproductive organs.

The primary hazards and other negative consequences of screening mammography include breast compression discomfort, patient recall for additional imaging, and false positive biopsies.

Thus, the correct option is C.

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Which term defines a liver disease that can be transmitted through sexual contact?
O mononucleosis
O chicken pox
O tuberculosis
hepatitis B

Answers

The liver disease that can be transmitted through close contact is Hepatitis B.

Close contact transmitted diseases

There are some disease that can be transmitted only through the exchange of body fluids such as blood or mucus that occurs during close contact. These diseases are collectively called close contact transmitted diseases.

A typical example is the Hepatitis B which is caused by the microorganism called hepatitis virus.

The hepatitis B viral disease permanently scars the liver leading to liver cancer or cirrhosis.

Mononucleosis, chicken pox and tuberculosis are all caused by invading of the body by microorganisms but are not gotten from close contact and they don't affect the liver.

Therefore, the liver disease that can be transmitted through close contact is Hepatitis B

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The following table provides the relevant information for a mutual fund over four quarters. 1st Quarter 2nd Quarter 3rd Quarter 4th Quarter Assets under management at the start of a quarter ($ million) 1 ? ? ? Holding-period return (%) 20 15 -5 10 Total assets before net inflows ? ? ? ? Net inflows ($ million) 0.2 0.1 -0.2 0.3 Assets under management at end of quarter ? ? ? ? (1) (6) Fill in the missing cells (marked as ?) using existing information. (2) (7) Using the holding period returns, compute the arithmetic average returns for the mutual funds over the four quarters. (3) (7) Using the holding period return, compute the geometric average returns for the mutual fund over the four quarters. At the school's holiday craft fair, Juan and his classmates get to make gingerbread houses. Their teacher divides a bag of licorice ropes evenly among the 8 students at the table. Each student gets 4 licorice ropes to decorate the roof of his or her gingerbread house.Which equation can you use to find the number of licorice ropes r that came in the bag? The resolution of radio telescopes suffers greatly from the large wavelengths of the light they are observing. What can be done to a radio telescope to improve its angular resolution?. A student would like to estimate the average number of text messages sent by cell-phone users in a day. Which ofthe following methods would provide a voluntary response sample?A. a survey of adults leaving a movie theaterB. a survey of all students in a statistics classC. a text poll provided during a nightly news broadcastD. a survey of every 10th person leaving a large shopping mall Explain how the structural timeline of the piece helps to build tension, thus heightening the story's comedic ending. If we have a line with points (0,0) (2,4) (4,8) what is the unit rate of this line? I need help with this please The word polyp is a shortened form of what 2 greek combining forms? help with 4, 5, and 6, please! Why is Max allowed to be in the same classes with Kevin? Using real-life examples, explain two differences between communism and democracy. Write at least three to fosentences to contrast the two types of government, and their application in world societies.WRITER find the indefinite integral. (use c for the constant of integration.) (2ti j 3k) dt jackson just received a settlement in a lawsuit that promises two future payments of $450, the first payment at the end of the 8th year, and the second at the end of the 13 year. jackson, being profligate, doesn't want to wait and instead wants instead to spend all of the settlement money today. what is the present value of his settlement assuming the interest rate of 5%? Describe two factors we should consider when evaluating an argument (discussed in Ch. 6 of THiNK: Critical Thinking and Logic Skills for Everyday Life.) Why are they important? a train whistle is heard at 305 hz as the train approaches town. the train cuts its speed in half as it nears the station, and the sound of the whistle is then 288 hz. what is the speed of the train after slowing down? speed of sound is 343 m/s. "hitlers words were like a scourge. when he spoke of the disgrace of germany, i felt ready to spring on any enemyglancing around, i saw that his magnetism was holding these thousands as one.i was a man of 32, weary of disgust and disillusionment, a wanderer seeking a cause.a yearner after the heroic without a hero. the intense will of the man, the passion of his sincerity, seemed to flow from him into me. i experienced a feeling that could be likened only to a religious conversion .i felt sure that no-one who heard hitler that night that he was the man of destinyi had given him my heart." according to the second document, why did kurt ludecke join the nazi party? What advantages did political stability bring to argentina, as opposed to less stable nations in latin america? select three choices. increased imports population growth increased exports new infrastructure european aid PLZ HELPYoung made several mistakes. He thought the third hieroglyph was part of the one for T, whereas it actually stood for the vowel O. The fourth hieroglyph, the lion, meant just L, the fifth meant M, and the last hieroglyph stood simply for S. In other words, the spelling in Egyptian was Ptolmis, not Ptolemaios.But Young got three out of the seven symbols right, which was a better score than any scholar before him had achieved.Young published his findings in an article written for the 1819 supplement to the Encyclopedia Britannica. He continued to work on the problem of the hieroglyphs in the years that followed, but made little headway in deciphering additional names and words. Why? Largely because he was working under a false assumption.Like countless other scholars over the centuries, Young still believed that most of the hieroglyphs must have a symbolic meaning. Only in special cases, such as foreign names, did he think that they were used to represent sounds.Because of this mistaken belief, Young put roadblocks in his own path. However, he had laid a solid groundwork for others in their attempts to decipher the hieroglyphs. And a young Frenchman, Jean-Francois Champollion, was ready to take up the challenge where Young had left off.The Riddle of the Rosetta Stone,James Cross GiblinBased on the passage, write two or three sentences explaining how the author feels about Thomas Young. Support your answer with examples from the reading. Why do you think that the naming of a new Supreme Court Justice is so important and often controversial? in a personal injury case involving a licensed teenage driver, a potential juror says he thinks that all teenagers are terrible drivers because they can't resist texting.