What are three methods for detecting radioactively labeled DNA in an experiment?

Answers

Answer 1

Answer:

Limitations. detect low abundance DNA binding proteins from lysates. test binding site mutations using many probe configurations with the same lysate. test binding affinity through DNA probe mutational analysis. non-radioactive EMSA possible using biotinylated or fluorescently labeled DNA probes.

Explanation:

Answer 2

Answer:

Proteins interact with DNA through electrostatic interactions (salt bridges), dipolar interactions (hydrogen bonding, H-bonds), entropic effects (hydrophobic interactions) and dispersion forces (base stacking). These forces contribute in varying degrees to proteins binding in a sequence-specific or non–sequence-specific manner.

Explanation:


Related Questions

9. Try to find the names of four cell organelles hidden in this maze. (Hint: The hadde
appear horizontally or vertically; forward or backward or even mixed up). Write
lines provided. For example: "NUCLEUS" in the last row, seven backward letters
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Answers

Answer: Please put more info

Explanation:

the table gives the standard free energy values for five of the enzymes of the citric acid cycle. the complete citric acid cycle is shown in the figure.

Answers

The primary source of energy for cells and an essential component of aerobic respiration is the citric acid cycle.

It is referred to as the Krebs or citric acid cycle. The cycle converts the acetyl coenzyme A (acetyl CoA) chemical energy into nicotinamide adenine dinucleotide (NADH) reducing force. The TCA cycle is a component of the broader glucose metabolism, which involves the oxidation of glucose to produce pyruvate, which is then further oxidized and enters the TCA cycle as acetyl-CoA.

The cycle's half of intermediates also serves as the starting point for pathways that lead to significant molecules such as fatty acids, amino acids, or porphyrins. The integrity of the cycle is compromised, and the cycle ceases to operate if any of these intermediates are so diverted. Only if anaplerotic (refilling) processes can replace the diverted intermediate or a subsequent intermediate that leads to oxaloacetate can vital energy production be restarted.

Hence, citric acid cycle require multiple enzyme to trap energy in molecular bond.

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the table gives the standard free energy values for five of the enzymes of the citric acid cycle. the

Coast Redwoods have been known to reach this size and age:

A. 50 feet tall; 10 feet diameter at breast height (DBH), and 200 years old


B. 380 feet tall; 30 feet diameter at breast height (DBH), and over 2,500 years old


C. 10 feet tall; 40 feet DBH, and 300 years old

Answers

Coast Redwoods have been known to reach  380 feet tall; 30 feet diameter at breast height (DBH), and over 2,500 years old.

So. option B is correct.

What are Coast Redwoods?

Coast Redwoods can be referred to as the sole living species of the genus Sequoia in the cypress family Cupressaceae.

The Coast Redwood is an evergreen, long-lived, monoecious tree living 1,200–2,200 years or more.

This species includes the tallest living trees on Earth, reaching up to 115.9 m (380.1 ft) in height (without the roots) and up to 8.9 m (29 ft) in diameter at breast height.

The leaves of the red coast Redwoods vary from being 15–25 mm (5⁄8–1 in) long and flat on young trees and shaded lower branches in older trees. The leaves has a structure of being scale like, 5–10 mm (1⁄4–3⁄8 in) long on shoots in full sun in the upper crown of older trees, with a full range of transition between the two extremes.

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Put the various atmospheres into the order they occurred from earliest (TOP) to latest (BOTTOM)

Put the various atmospheres into the order they occurred from earliest (TOP) to latest (BOTTOM)

Answers

Answer:

1. A thin layer of hydrogen and helium that was blown off by solar wind.

2. heavily water vapor.

3. mostly carbon dioxide and methane.

4. mostly nitrogen and oxygen.

Hi if the answer is wrong am sorry I tried but if it is correct then I'm happy I helped

a species that plays a major role in determining the structure of its ecological community is a) a dominator species b) a predator c) the largest species d) the most abundant species in the community e) a keystone species

Answers

The correct option is E ; A keystone species ,  A keystone species is important in an ecosystem because it is the species whose presence has an impact on all other species in the ecosystem.

A keystone species is always a dominating predator that feeds on the population of prey. If it is eliminated, the prey population would explode, reducing population diversity. Bison, prairie dog, and so forth.

The term "niche" in ecology refers to the role that an organism plays in a group. A species' niche includes both the physical and environmental characteristics

it demands (such as temperature or topography) as well as its interactions with other species (like predation or competition).

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How do humans change the balance of the ecosystem

Answers

Answer:

Humans may destroy natural landscapes as they mine resources and urbanize areas. This is detrimental, as it displaces residing species, reducing available habitats and food sources. This can negatively effect an ecosystem because the introduced species may outcompete native organisms and displace them

Explanation:

Answer:

Humans impact on the natural environment has reached unprecedented levels. Humans are present on all continents: almost allecosystems have been modified by human activities through habitat loss and fragmentation, overpolitation, and invasive species

Explanation:

IS THIS CORRECT!!! HURRY ASAP!!!

IS THIS CORRECT!!! HURRY ASAP!!!

Answers

The correct matches of the phases of the menstrual cycle are given below:

Flow phase: B, E

Follicular phase: F, D,

Luteal phase: A, C

What is the menstrual cycle?

The menstrual cycle refers to the cycle period in which the endometrial lining of the uterus is shed and a new lining is produced. It usually lasts for a period of 28 days.

The phases of the menstrual cycle are as follows:

Flow phase:

The endometrium tears away, blood vessels are ruptured and bleeding occurs.The endometrium begins repair around day 5 and becomes thicker.

Follicular phase:

The anterior pituitary gland begins the production of LH and FSH which stimulates follicle maturity in the ovary.The oocyte matures to an ovum, a large amount of LH causes the follicle to rupture and ovulation occurs.

Luteal phase:

The corpus luteum produces high amounts of progesterone and some estrogen, which keep levels of LH and FSH low.The corpus luteum breaks down, and it no longer produces progesterone and estrogen. This results in a rapid decrease in progesterone and estrogen levels.

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you have isolated and grown a pure culture of a prokaryotic cell type. the first step in identification is a(n)

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By analyzing the results of the Gram stain, you can determine if your prokaryotic cell type is Gram-positive or Gram-negative,

The first step in identification of a prokaryotic cell type is a Gram stain. This is a crucial procedure that helps to classify bacteria based on their cell wall structure. The Gram stain differentiates between Gram-positive and Gram-negative bacteria by using a series of dyes and chemicals.

Here's a step-by-step explanation of the Gram staining process:

1. Prepare a bacterial smear by spreading a small sample of your pure culture onto a microscope slide.
2. Heat-fix the bacterial smear by passing the slide through a flame to help the cells adhere to the slide and kill the bacteria.
3. Apply crystal violet dye to the slide, staining all cells a purple color.
4. Rinse off the excess crystal violet dye with water.
5. Apply iodine, which acts as a mordant, forming a complex with the crystal violet dye.
6. Decolorize the slide using an alcohol or acetone solution, which will remove the dye from Gram-negative cells, leaving them colorless.
7. Rinse the slide with water to stop the decolorization process.
8. Apply a counterstain, such as safranin, to the slide. This will stain the now-colorless Gram-negative cells a pink or red color, while the Gram-positive cells will remain purple.
9. Rinse the slide again and allow it to air dry or gently blot it dry with a paper towel.
10. Examine the slide under a microscope using an oil immersion objective to observe the stained cells.

By analyzing the results of the Gram stain, you can determine if your prokaryotic cell type is Gram-positive or Gram-negative, which is the first step in identification.

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All of the following are negative consequences of enhanced greenhouse gas effect EXCEPT.


1) decreased temperatures

2) increased UV insolation

3) melting glaciers and ice caps

4) increasing sea levels

Answers

All of the following are negative consequences of enhanced greenhouse gas effects except melting glaciers and ice caps as it is a part of a positive feedback loop.

This positive feedback loop, which also actively raises temperatures, especially in the Arctic areas, has a substantial impact on the local ecology.

In comparison to other planets, the earth's temperature is one of its most striking and distinctive features. There are many different biological forms on the globe, and they all survive. The current increase in global warming on Earth is a result of a number of factors, including human activity, industrial waste, and trapped greenhouse gases.

Global warming has caused the ice sheets to begin to melt. As the white snow sheets melt away, the black ocean water, rocks, and flora hidden behind them are more easily seen; this phenomenon is regarded to create a positive feedback loop. Since the ice sheets are white, most of the incident light is reflected when they melt. The local ecology is significantly impacted by this positive feedback loop, which also actively elevates temperatures, particularly in the Arctic regions. Darker ocean water has a tendency to absorb more radiation, which boosts temperatures even more and speeds up global warming.

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Write the lyrics of O Ramji bada dukh dina​

Answers

Sa ga re ga sa sa ga re
Ga sa ga re pa ga re
Re ma pa ni dha, sa ga ma dha pa
Ni re ga pa ma re ma ga sa ga re ni re sa
O Ramji, bada dukh di na
Tere Lakhan ne bada dukh di na - 2
Sudh budh bisraayi meri neend churaayi
Mera mushkil kar diya jeena
Bada dukh di na
Tere Lakhan ne bada dukh di na
O Ramji, bada dukh di na
Voh deepak hai, main jalti hoon - 2
Voh hansta hai, main roti hoon
Mere pyaar ki kadar usse hogi magar
Mujhe zaher padega peena
Bada dukh di na, bada dukh

WHATS THE ANSWER ?????

WHATS THE ANSWER ?????

Answers

Answer:

Antigens are secreted by the endocrine system

Explanation:

The main job of the innate immune system is to fight harmful substances and germs that enter the body, for instance through the skin or digestive system. The adaptive (specific) immune system makes antibodies and uses them to specifically fight certain germs that the body has previously come into contact with

List 3 facts about HIV/AIDS

Answers

Answer:

Fact: HIV and AIDS are problems in the United States. ...

Fact: HIV is not the same as AIDS. ...

Fact: HIV tests are reliable. ...

Fact: HIV cannot be cured. ...

Fact: There is no vaccine to prevent HIV. ...

Fact: People with HIV should start HIV medicine right away.

Explanation:

hope it help!

What could create the need for a secondary succession?
i need the answer

Answers

Answer:

Particularly common types of secondary succession include responses to natural disturbances such as fire, flood, and severe winds, and to human-caused disturbances such as logging and agriculture. In secondary succession, the soils and organisms need to be left unharmed so there is a way for the new material to rebuild.

Answer:

Because you never wait.

Explanation:

Because this is correct.

Identify which of the blood vessels
are blue in color and explain why,

Answers

Answer:

Arteries deliver blood to the body, and veins return blood to the heart. Veins appear blue because of lack of oxygen and the way the light hits our body, and because our eyes play tricks on us.

Explanation:

This is what I have read from different sources and hope it helps you.

What is the relationship between III-1 to III-2

What is the relationship between III-1 to III-2

Answers

Answer:

III 2 IS THE HUSBAND OF III1

help ASAP will give BRAINLIEST
4. Which biomolecule is composed of nucleotides?
Carbohydrates
Nucleic Acid
Protein
Lipids​

Answers

Answer:

Nucleic Acid

Good luck with your schooling :)

Answer= Nucleic Acid

Nucleic Acids are biomolecules containing hydrogen, oxygen, nitrogen, carbon and phosphorous.

you are thinking about the effect of reproduction on relatedness. please order the following sibling sisters by their degree of relatedness from highest to lowest relatedness. the sisters are: (1) fraternal (dizygotic) twins (2) offspring of the same mother but two unrelated fathers (3) offspring of a parthenogenetic mother (4) identical (monozygotic) twins (5) adopted from unrelated parents group of answer choices 1,2,3,4,5. 5,4,3,2,1. 3,1,4,2,5. 3,4,1,2,5. 3,4,1,5,2.

Answers

The correct order of the sisters based on their degree of relatedness from highest to lowest is: 4, 3, 1, 5, 2.

Explanation:
1. Identical (monozygotic) twins (4) have the highest degree of relatedness as they share 100% of their genetic material.
2. Offspring of a parthenogenetic mother (3) come next, as they are genetically identical to their mother.
3. Fraternal (dizygotic) twins (1) share approximately 50% of their genetic material, as they are born from the same mother but are fertilized by two different sperm cells.
4. Adopted sisters (5) have no genetic relation and share no genetic material.
5. Offspring of the same mother but two unrelated fathers (2) have the lowest degree of relatedness, as they share only their mother's genetic material but not their fathers'.

Conclusion:
The correct order of the sisters from highest to lowest relatedness is: 4, 3, 1, 5, 2. Identical twins are the most closely related, followed by offspring of a parthenogenetic mother, fraternal twins, adopted sisters, and offspring of the same mother but unrelated fathers.

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In mice, black fur (W) is dominant over white fur (w), and black eyes are dominant (R) over red eyes (r). The two genes are located on different chromosomes.A male mouse with black fur and black eyes mated with a female mouse with white fur and red eyes. Out of 64 offspring, 30 had black fur and black eyes, and 34 had white fur and black eyes. A male mouse heterozygous for both fur and eye colour mated with a female mouse heterozygous for both traits.Out of 64 offspring, how many mice can be expected to be heterozygous for both fur and eye colour? Select one: a. 16 b. 12 c. 36 d. 1 e. 48 f. 4

Answers

Answer:

To solve this problem, we need to first determine the genotypes of the parent mice and then use a Punnett square to predict the genotypes and phenotypes of their offspring.

The male mouse with black fur and black eyes is WWRR (homozygous dominant for both traits) and the female mouse with white fur and red eyes is wwrr (homozygous recessive for both traits).

When these two mice mate, their offspring will all be WwRr (heterozygous for both traits) because each parent can only pass on one allele for each gene.

Out of the 64 offspring, we know that 30 had black fur and black eyes (WWRR or WWRr), and 34 had white fur and red eyes (wwrr). This means that the remaining offspring (64 - 30 - 34 = 0) must have had black fur and red eyes (Wwrr).

When the heterozygous male (WwRr) mates with the heterozygous female (WwRr), we can use a Punnett square to predict the genotypes and phenotypes of their offspring:

| | W | w |

|---|---|---|

| R | WWRR | WWRr |

| r | WwRR | WwRr |

So, out of the 64 offspring, we can expect 16 (25%) to be heterozygous for both fur and eye color (WwRr). Therefore, the answer is a. 16.

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Explanation:

the structure of a biological membrane is best described by the _______ model.

Answers

The structure of a biological membrane is best described by the Fluid Mosaic Model. The biological membrane is a thin sheet-like structure that separates and protects the cell from the external environment. The membrane plays a crucial role in the cellular processes such as transportation of molecules, cell signaling, and recognition.

Fluid Mosaic ModelThe Fluid Mosaic Model of the biological membrane was first proposed by Singer and Nicolson in 1972. This model is the most widely accepted model of the biological membrane and describes the dynamic nature of the cell membrane. According to the model, the membrane consists of a phospholipid bilayer in which proteins are embedded. The phospholipids in the membrane are amphipathic molecules, meaning they have a hydrophobic and a hydrophilic end. The hydrophobic tails of the phospholipids are arranged facing each other in the center of the membrane while the hydrophilic heads are facing the external and internal environment.

The proteins present in the membrane are responsible for various functions such as transportation, recognition, and cell signaling. The mosaic nature of the model arises from the variety of proteins and lipids present in the membrane. The model also suggests that the membrane is in a fluid state and the lipids and proteins move around freely within the membrane.

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(b) Through the process of transpiration, water is brought into a plant through
its roots and carried to the leaves, from which it evaporates. What structure
brings water into the leaf from the roots?

Answers

The xylem is a special type of cell that brings water from roots to leaves through the stem.

Plant stems include a type of cell called an xylem. These cells create long, thin tubes that connect the roots to the stems and then to the leaves. Their duty is to transport water upward from the roots to every part of the plant. 

Capillary action is the mechanism through which water flows up the xylem. Water molecules enjoy clumping together. Water is drawn up through the tubes by capillary action because extremely small components of water, known as molecules, are attracted to the molecules that make up the tube.

The top water molecules are drawn up the tube, and the water molecules below them are drawn along because they are attracted to the water molecules above them.

When plants have more water in their leaves than they need, they expel it through a process known as transpiration. Water evaporates into the air from small pores in the surfaces of leaves during transpiration. Stomata are little tiny holes.

As water molecules evaporate off plant leaves, they attract water molecules that remain in the plant, assisting in the movement of water up through the stems from the roots. Water is delivered from the bottom to the top of a plant by transpiration and capillary action.

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15. How can a capillary shift from a predominantly filtering role at one time to a predominantly reabsorbing role at another

Answers

The capillary is an important part of the circulatory system, as it shifts between a predominantly filtering role and a predominantly reabsorbing role.

It begins with the capillary filtering blood from the arterial end, allowing small substances to pass through its walls. This process is known as filtration, and it is essential for the removal of waste products and excess fluid from the body.

As the blood continues to move through the capillary, it will begin to reabsorb certain substances. This is known as reabsorption, and it is necessary to ensure that the body has the right balance of essential nutrients and fluids. This process occurs in the venous end of the capillary.

The capillary's ability to shift between a filtering and reabsorbing role is essential for maintaining homeostasis in the body. By filtering and reabsorbing in a balanced and regulated manner, the capillary helps to keep the body functioning properly.

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A mosquito can transmit a viral pathogen into humans. What is the mode of transmission of the virus in this instance

Answers

vector… i think that’s right

in what ways are land organism most dependent on ocean organism

Answers

Living organisms are dependent on the soil in the following ways:

Soil plays an important role in a biogeochemical cycle, which is the main source of recycling nutrients from the atmosphere to soil and then to water.
All the plants (even aquatic), on which almost all the organisms depend directly or indirectly for food, grow in the soil, which provides them with nutrients and water for development.

what happens to a rock when it is weathered?

Answers

erosion........................

Explanation:

what happens to a rock when it is weathered?

Weathering is the breaking down or dissolving of rocks and minerals on Earths surface. Once a rock has been broken down, a process called erosion transports the bits of rock and minerals away. ... Water, ice, acids, salts, plants, animals, and changes in temperature are all agents of weathering.

Use one of the properties of water to explain why, on a very hot and sunny day, outdoor swimming pools are able to stay cool.

Answers

Answer:

what are we supost to do

Explanation:

Answer:

This is due to evaporation. When you get out of the swimming pool, the water is evaporating from your skin. This process is takes a lot of energy, and it takes that energy in the form of heat from your body.

the doctrine of specific nerve energies involves the stimulation of

Answers

The Doctrine's central tenet is that the direct objects of feeling are the actions of sensory nerves, rather than properties external to the body.

The Doctrine of Specific Nerve Energies has had and continues to have a huge influence on perception physiology, psychology, and philosophy. In layman's terms, the Doctrine asserts that we sense the activity of our nerves first and foremost, rather than qualities in the external environment. The initial recognition of a stimulus as a result of physical stimulation of a receptor in a sense organ is referred to as sensation (e.g., eye, ear, etc.). The integration and interpretation of sensory information is referred to as perception.

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The release of one phosphate group from ATP powers the reactants in the cell which phosphate is the one that is released from ATP

Answers

Answer:

ADP

Explanation:

Your question: The release of one phosphate group from ATP powers the reactants in the cell which phosphate is the one that is released from ATP

My answer: ADP

Why is my answer correct?

If a cell needs to spend energy to accomplish a task, the ATP molecule splits off one of its three phosphates, becoming ADP (Adenosine di-phosphate) + phosphate. The energy holding that phosphate molecule is now released and available to do work for the cell.

Hope this helps! :)

how does natural selection led to the adaptation of the cliff swallow population along l-80?

Answers

Answer:

0110011101101111001000000110011001110101011000110110101100100000011110010110111101110101011100100010000001110011011001010110110001100110

Explanation:

which type of rna is involved in protein synthesis

Answers

ribosomal rna i’m pretty sure

List out 3 caste of honey bees with their description​

Answers

Answer:

There are three castes in honey bees:

dronesworkersqueens.

Explanation:

There are three castes in honey bees:

Drones-Male honey bees are drones. The head and thorax of the drone are bigger than the females.Queens-Honey bee queens are the species' reproductive women.Workers-Workers' sweet bees are generally non-reproductive women.
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