what are the 7 steps of design for a molecular cloning experiment (in order)
Answers
The 7 steps of design for a molecular cloning experiment in order are: Selection of vector, Restriction digestion, Ligation, Transformation, Selection, Screening, Verification.
Selection of vector and restriction enzymes: The first step is to choose a suitable vector and restriction enzymes for cloning the DNA fragment of interest.
Restriction digestion: The DNA fragment of interest and the vector are both cut with the same restriction enzyme, which generates compatible ends that can be ligated together.
Ligation: The cut DNA fragment and the vector are mixed together with DNA ligase to form a recombinant DNA molecule.
Transformation: The recombinant DNA molecule is introduced into competent bacterial cells, which take up the DNA and become transformed.
Selection: Bacterial cells that have taken up the recombinant DNA molecule are selected based on the presence of a selectable marker on the vector.
Screening: Clones that contain the correct insert are identified by screening with a probe or by PCR using primers specific to the insert.
Verification: The final step is to confirm that the cloned DNA fragment is the correct sequence and orientation by sequencing the DNA or performing restriction mapping.
These steps ensure successful cloning of the DNA fragment of interest into the vector of choice and confirmation of the correct insertion.
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Related Questions
Mini Brain ProjectDirections: Conduct research on a selected brain area or structure. Areas and structures available for research include: frontal lobe, parietal lobe, temporal lobe, occipital lobe, medulla, pons, cerebellum, reticular formation, thalamus, hypothalamus, limbic system, cerebrum, hippocampus, amygdala, corpus callosum, Broca’s area, OR Wernicke’s area. Conduct research using scholarly Internet sources, library sources, and the textbook. What are its major functions? What techniques are used to view or measure it?What other structures is it near?What other structures help or perform similar functions?
Answers
Parietal lobe is one of the main lobes in the brain which is located at the upper back area in the skull. It process sensory information that it accepted from the outside world that is associated to touch, taste, and temperature. The techniques used to view the parietal lobe are Positron emission tomography (PET) and functional magnetic resonance imaging (fMRI).
Parietal lobe is near the top and center of the cerebral cortex just at the back of the frontal lobe and at the top of the occipital and temporal lobes.
The occipital lobe help the parietal lobe in visual perception and processing including spatil navigation and reasoning.
In Mendel's F2 generation of the purple and white flower crossing, the dominant to recessive ratio was
1:3:1
3:1
1:1
9:7
9:3:3:1
Answers
In Mendel's F2 generation of the purple and white flower crossing, the dominant to recessive ratio was b. 3:1. therefore, option b. 3:1 is correct.
This means that out of every four plants in the F2 generation, three had the dominant trait (purple flowers) and one had the recessive trait (white flowers).
This ratio is the result of Mendel's law of segregation, which states that the two alleles of a gene (in this case, the P and p alleles for flower color) separate from each other during the formation of gametes (pollen and eggs) so that each gamete receives only one allele.
The 3:1 ratio is expected when both parents are heterozygous for the trait, meaning they each carry one dominant allele and one recessive allele.
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what cell type remains after an adaptive immune response and is responsible for maintaining the steady-state level of pathogen-specific antibody in the circulation?
Answers
The cell type that remains after an adaptive immune response and is responsible for maintaining the steady-state level of pathogen-specific antibody in circulation is long-lived plasma cells.
Adaptive immunity is a kind of immunity that is made against infectious diseases.
This type of immunity produces a strong response against pathogens and hence is pathogen-specific. The plasma cells that are longed lived produce antibodies as well as memory cells against the specific microbes.
These plasma cells are longed lived so that they can recognize the specific pathogen whenever it invades the body.
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Brain-damage-produced deficits in language-related ability are generally referred to as. A) aphasia. B) aphagia. C) apraxia. D) dyslexia. E) dementia.
Answers
The brain-damage-produced deficits in language-related ability are generally referred to as aphasia.
Aphasia is a neurological disorder that affects a person's ability to communicate, comprehend, and express language. It is usually caused by damage to the left hemisphere of the brain, where the language centers are located. The severity of aphasia can range from mild language difficulties to complete loss of language ability. There are several types of aphasia, including expressive aphasia, receptive aphasia, and global aphasia, each with its own set of symptoms and language deficits. Aphasia can be treated with various speech therapy techniques, and the prognosis for recovery depends on the severity and location of the brain damage.
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True or false: bioinformatics can be used to find cause-and-effect relationships between an individual's overall genetic makeup and resulting genetic disorders.
Answers
The answer is true. Bioinformatics is described as the use of computational and analytical tools to capture and understand biological data. It is a multidisciplinary field that combines computer science, mathematics, physics, and biology.
Utilizing computers to gather and analyze biological data, particularly in the areas of genetics and genomics, is referred to as bioinformatics. A bioinformatics example is the use of computer analysis on the Human Genome Project, which has catalogued the three billion base pairs of the human DNA system. In other nations, it is one of the industries with the highest salaries. In India, the sector is likewise expanding quickly.
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Describe the anthropogenic particulates that could be found at a coal-burning power plant.
Answers
Anthropogenic particulates found at a coal-burning power plant can include sulfur dioxide (SO2), nitrogen oxides (NOx), volatile organic compounds (VOCs), and particulate matter (PM).
SO2 is an acidic gas that can react with other pollutants in the atmosphere to form small particles. NOx, a pollutant gas created during combustion, contributes to the formation of smog and acid rain. VOCs are emitted as gases from solids or liquids and can cause serious health problems. PM is made up of a mixture of solid particles and liquid droplets that are small enough to be inhaled into the lungs and cause serious health issues. All of these pollutants contribute to the degradation of air quality and are a major concern for communities near coal-burning power plants.
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Each enzyme is responsible for ______ _____________________ that particular reaction that occurs in the cell. The _____________ _________ is where the reactant binds to enzymes. Enzymes can be reused until they become___________________usually changes to pH and temperature.
Answers
Answer:
speeding up /catalysing
active site
inactive /destroyed
Explanation:
A population of wild turkeys lives in a forest ecosystem. The population’s size is near the carrying capacity of its environment.
Which of the following best describes the forest ecosystem’s carrying capacity for the wild turkey population?
Choose 1 answer:
Choose 1 answer:
Answers
1. Shreya's grandmother has pain in her knees. Her neighbor said it could be because of the wearing off of her knees. Do you think she is right? Which part of her knee may have worn off?
Answers
Arthritis or rheumatoid arthritis is a prevalent condition among the elderly.
The knee is one of the largest and most intricate joints in the body. The knee is the joint that links the femur (thigh bone) to the shin bone (tibia). The smaller bone that runs alongside the tibia (fibula) and the kneecap are the extra bones that make up the knee joint (patella).
Osteoarthritis is the most common kind of arthritis that affects the knee. Osteoarthritis is a degenerative disease in which the cartilage in the joint gradually deteriorates. It primarily affects persons in their forties and fifties. Osteoarthritis may be caused by excess stress on the joint such as repeated injury or being overweight.
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Choose all of the chemical changes: Group of answer choices
A burning wood
B melting ice
C making snow cones
D dissolving salt
E cooking eggs
F rusting car fender
Answers
dissolving salt
Explanation:
i do not know
If a cell underwent mitosis, and its daughter cells were immediately exposed to chemicals that damaged the dna, at which stage of the cell cycle checkpoint would you predict the cell would arrest?.
Answers
Before mitosis, the cell undergoes through a previous stage known as interphase, which is composed of the G1, S, and G2 stages. The stage of the cell cycle checkpoint at which the cell would arrest would be the G1 stage of the interphase.
What is the interphase?The whole cell cycle is composed of two main phases: the interphase and the cell division phase. Cell division might be either mitosis or meiosis.
The interphase occurs before cell division and is the stage during which the cell prepares to divide, which means its content must be in the proper proportions (duplicated) to produce daughter cells with the same intracellular content.
Three stages compose the interphase G1, S, and G2.
After the interphase, the cell undergoes division. When the cell ends its division, the produced daughter cells immediately begin the process again, starting with the interphase.
If a cell underwent mitosis, and its daughter cells were immediately exposed to chemicals that damaged the dna, the stage of the cell cycle checkpoint at which the cell would arrest would be the G1 stage of the interphase.
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H Q₁: Define and illustrate the following ther inologies? I 1) The contact angle 1 1 2) pressure angle I 3) Coriolis force(effect) 4) scotch yoke mechanism 5) The Cam angle H 1 H
Answers
The contact angle is the angle formed between a liquid droplet and a solid surface at the point where they come into contact. It is a measure of the wettability of the surface by the liquid.
Pressure angle: The pressure angle is an important parameter used in gear design. It is the angle between the direction of the force exerted on a gear tooth by another gear and a line tangent to the pitch circle of the gear. Coriolis force (effect): The Coriolis force, also known as the Coriolis effect, is an apparent force that acts on a moving object in a rotating reference frame.It is caused by the rotation of the Earth and can be observed in various phenomena, such as the deflection of moving objects (e.g., projectiles or air currents) on the Earth's surface.Scotch yoke mechanism: The scotch yoke mechanism is a type of mechanical linkage used to convert rotary motion into linear motion. It consists of a sliding yoke or slider that is driven by a rotating crank.The pressure angle refers to the rotational position of a camshaft in an engine. A camshaft has lobes or cams that are used to control the opening and closing of valves in the engine.
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describe how each of the following can impact stroke volume.
- damage to the conduction system of the heart
-a blockage in one of the coronary arteries
Answers
Damage to the conduction system of the heart and a blockage in one of the coronary arteries can both impact stroke volume, which is the amount of blood pumped by the heart with each contraction.
Damage to the conduction system of the heart: The conduction system of the heart is responsible for coordinating the electrical signals that regulate the heartbeat. Damage to the conduction system can disrupt the normal electrical signals and result in irregular heartbeats (arrhythmias) or complete heart block, where the electrical signals are completely blocked and the heart cannot contract effectively. This can lead to a decrease in stroke volume as the heart may not pump blood efficiently, resulting in a reduced amount of blood being ejected with each contraction.Blockage in one of the coronary arteries: The coronary arteries are responsible for supplying oxygen-rich blood to the heart muscle itself. When one of these arteries becomes blocked, usually due to a buildup of plaque or a blood clot, it can restrict blood flow to a portion of the heart muscle. This can result in decreased oxygen delivery to that area of the heart, leading to impaired cardiac function and reduced stroke volume. If the blockage is severe and prolonged, it can even result in a heart attack, which can further impact stroke volume due to damage to the heart muscle.Both of these conditions can ultimately decrease stroke volume, which can have a significant impact on overall cardiac function and the ability of the heart to effectively pump blood to meet the body's needs. It is important to seek appropriate medical care and treatment for any conditions that may affect the conduction system of the heart or the coronary arteries to minimize their impact on stroke volume and overall cardiovascular health.
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1. What happens to the rubber band when you pulled? a. The rubber band stretches c. Nothing happens to the rubber band. b. The rubber band suddenly cut out. D. all of the above 2. Using this model identify the type of fault. a. Strike-slip fault. b. Normal Fault c. Reveres Fault d. all of the above 3. This is the location on the surface of the Earth directly above the focus. a. Focus b. Fault c. Epicenter d. All of the above 4. It is where the earthquake starts. a. Focus b. Fault c. Epicenter d. All of the above 5. Which of the following is not related to earthquake? A. Ground shaking B. Fault C. After shocks D. Typhoon 6. Which of the following are safer place during an earthquake? A. Inside the house B. Outside in an open area C. Inside the car D. Under a tre Items 7-10 refer to the diagram below. C B GA
Answers
Answer:
d
Explanation:
Why is soil erosion more common in areas that lack adequate vegetation?
Select one:
O a. Because gravity pulls on the plants and increases erosion.
Ob.
Because plant roots help keep the soil in place.
O c.
Because the lack of adequate vegetation affects the soil pressure and erosion rate.
Od. Because soil erosion is stopped by reduced vegetation.
Answers
Answer:
B: Because plant roots help keep the soil in place.
Brainliest?
Which of the following has to
occur in order for mammals to
create offspring?
A. fertilization
B. self-reproduction
C. mutation
D. self-fertilization
Answers
what is returning water, electrolytes, and nutrients to the bloodstream called?
Answers
The procedure that removes solutes and water from the filtrate and returns them to your bloodstream is known as tubular reabsorption.
The reabsorption of electrolytes, water, and preserving the acid-base balance are all crucial functions of the renal tubules. Reabsorption is the process of returning chemicals, ions, and water from the glomerular filtrate to the blood that the body needs to maintain homeostasis.
The right balance of water and electrolytes is maintained and controlled by the kidneys. High pressure blood enters a glomerulus. Via tiny holes in the glomerulus, a considerable portion of the blood's fluid component is filtered, leaving behind the majority of big molecules like proteins and blood cells.
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There are more primary producers than what
Answers
Answer:
Primary Consumers.
Explanation:
If an organism is multicellular, it probably has
A.) Prokaryotic cells
B.) Eukaryotic cells
C.) Cancer cells
D.) All of the above
Answers
Answer:
B. Eukaryotic cells
Explanation:
Prokaryotic cells are only seen in unicellular organisms as bacteria and cancer cells are just cells where there has been an error in division or a mutation.
If an organism is multicellular, it probably has - option B.) Eukaryotic cells.
Cells are of mainly two types prokaryotic cells and eukaryotic cells. The main difference between eukaryotic and prokaryotic cells is that:
Eukaryotic cells have a nucleus in which genetic material is stored enclosed by a nuclear membrane.All prokaryotic cells only form single-celled (unicellular) organisms without a true nucleus or membrane-bound structures, examples of prokaryotes are bacteria and archaea. Organisms with eukaryotic cells are called eukaryotes that can be either multicellular such as animals, plants, fungi, and protists, or single-celled as well. So, all multicellular organisms are eukaryotes..Thus, If an organism is multicellular, it probably has - option B.) Eukaryotic cells.
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Adult female monkeys from one population breed with male monkeys of a nearby population. The introduction of new alleles into the first population's gene pool is an example of
Answers
Answer:
The introduction of new alleles into the gene pool of the first population, from another population, is an example of gene flow.
Explanation:
In population genetics, gene flow consists of the passage or transfer of gene alleles between populations, a process that is related to the migratory dynamics of a population.
When gene flow occurs, a population can gain new genetic material or lose it, which is reflected in traits or characteristics of that population.
If an adult monkey interbreeds with an adult monkey from another population there will be a gene flow that will probably introduce specific trait information into the gene pool of their respective populations.
a rapid process of reproduction is an advantage but little or no diversity is a disadvantage of?
Answers
Answer:A rapid process of reproduction can be an advantage in certain circumstances, such as in rapidly changing or unpredictable environments, where being able to produce large numbers of offspring quickly can increase the likelihood of at least some of them surviving and passing on their genes.
Explanation: Without genetic diversity, a population may be less able to adapt to changing conditions or resist diseases and parasites, which can ultimately lead to decreased fitness and survival. Therefore, while rapid reproduction can provide some benefits, it's important to maintain genetic diversity within a population to ensure its long-term survival and success.
1. Which is not a characteristic of most plants?
a. possess tissues
C. possess plastids
d. are consumers
b. are eukaryotic
Answers
majority of plants use photosynthesis, its rare that a plan consumes, venus fly trap is one of the only plants that consumes
The text below was part of an article in a teenage magazine:
‘Young people today are far too materialistic.’
Do you agree?
Answers
Answer:
That's um...well how do I say this, terrible. They're saying it's a horrible thing but I think not, everyone does different things and I respect that. So it's best not to judge someone by what they're doing even in a magazine.
What can engineers do to protect ecosystems from invasive species ?
will mark brainliest !!!!
Answers
Answer:
By extensively filtering the water in a lake or a bay they can clear it enough to increase light penetration thus affecting benthic macroalgal communities by facilitating growth. There are numerous examples of animals and plants that function as ecosystem engineers.
In ecosystems engineers by altering their receptive environment, invading species have the potential to play a dominant role as ecosystem engineers and can have an ecosystem-level impact by hindering or facilitating other species, whether invasive or native.
What are ecosystems engineers?Ecosystem engineers are organisms that alter the physical condition of biotic or abiotic materials to affect the availability of resources (other than their own) to other species. By doing this, they alter, preserve, or even create new environments.
They can clear the water in a lake or bay sufficiently to promote light penetration, which will influence benthic macroalgal ecosystems by promoting growth.
Therefore, there are many instances of plants and animals that act as ecosystem engineers.
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Mrs. Franklin loves teaching science and thinks about it all the time. While walking one day, she steps on a warm, fresh piece of used gum. The gum flattens and sticks to her shoe. When she lifts her foot, she sees long strings of gum between her shoe and the sidewalk. She thinks, “What a great demonstration of the properties of metals!”
What properties of metals would Mrs. Franklin most likely teach about with this demonstration?
conductivity and luster
ductility and malleability
malleability and reactivity
conductivity and ductility
Answers
Answer: Ductility & malleability
Explanation: Ductility is the capability of a material to be deformed or molded without breaking and malleability the ability for a material to be reshaped without breaking. Stepping on gum exemplifies both qualities.
Answer:
malleability and reactivity
with very few exceptions, all energy that enters biological systems ultimately comes from the
Answers
describe how muscles of respiration change their activity between inhalation and exhalation
Answers
The muscles of respiration change their activity between inhalation and exhalation by altering the volume of the thoracic cavity.
The muscles of respiration change their activity between inhalation and exhalation by altering the volume of the thoracic cavity. During inhalation, the diaphragm and external intercostal muscles contract, causing the thoracic cavity to expand and the lungs to fill with air. The accessory muscles, such as the sternocleidomastoid and scalene muscles, also assist in this process. During exhalation, the diaphragm and external intercostal muscles relax, and the elastic recoil of the lungs and thoracic cage forces air out of the lungs. The internal intercostal muscles and abdominal muscles may also contract to increase the pressure in the thoracic cavity, further aiding in the exhalation process. The coordination of these muscles ensures efficient ventilation of the lungs and delivery of oxygen to the body's tissues.
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Define photophosphorylation?
Answers
The photophosphorylation is the synthesis of ATP from ADP and phosphate in the plants using the light energy absorbed during the photosynthesis process.
For what purpose do lipids accumulate in connective tissue?
Answers
Answer:
the lipids accumulate in connective tissue is used for complex cleaning purpose
Lipid accumulation product (LAP) is an index, which combines waist circumference (WC) and triglyceride (TG) reflecting lipid accumulation. Lipids are available to the body from three sources. They can be ingested in the diet, stored in the adipose tissue of the body, or synthesized in the liver. Lipid storage (especially sphingolipid and unesterified cholesterol) is found in liver and spleen. In the brain, glycolipid storage predominates, often with some decrease in myelin white matter. The condition is inherited as an autosomal recessive trait.
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Which of the following is not an environmental function of wetlands?
Group of answer choices
absorbing and removing pollutants from water
trapping carbon that would otherwise be released into the air
controlling floods
increasing runoff
Answers
Answer:
increasing runoff
Explanation: