sodium dodecyl sulfate (sds) contains a 12-carbon tail attached to a sulfate group and is used in denaturing gel electrophoresis of proteins. numerous sds molecules will bind to the exposed hydrophobic regions of denatured proteins. how does the use of sds in this experiment allow for the separation of proteins?
Answers
The use of sodium dodecyl sulfate (SDS) in denaturing gel electrophoresis allows for the separation of proteins by standardizing their migration based on size.
SDS is an anionic detergent that binds to denatured proteins, coating them with a negative charge proportional to their length. This negative charge masks the intrinsic charge of the protein, making it linear and negatively charged. As a result, the migration of proteins in the gel is solely determined by their size, with smaller proteins moving faster and farther than larger ones.
SDS-PAGE (polyacrylamide gel electrophoresis) separates the protein mixture into distinct bands according to their molecular weight. Therefore, the use of SDS in the experiment enables the separation of proteins based on their size, facilitating their analysis and identification.
To learn more about gel electrophoresis here
https://brainly.com/question/30051368
#SPJ4
Related Questions
What are themes of the 1st chapter of the War of the Worlds by H. G. Wells?
Answers
Answer:
Social Darwinism and Evolution
Explanation:
This are the themes of the 1st chapter of the War of the Worlds by H. G. Wells.
following habituation to one stimulus, the tendency for a second stimulus to arouse new interest (often used to testwhether infants can discriminate between stimuliDishabituation
Answers
Habituation is a decrease in response to a repeated stimulus. In infants, this can be observed through a decrease in attention or interest in a particular object or sound. Dishabituation refers to the reversal of this process, where a second stimulus is presented after habituation and it elicits a new response or renewed interest.
Dishabituation refers to the process where, after habituation to one stimulus, a second stimulus is introduced which arouses new interest. This concept is often used to test whether infants can discriminate between stimuli.
1. Habituation: Infants are exposed to a stimulus (e.g., a sound or image) repeatedly until their response decreases, indicating that they have become habituated to it.
2. Second stimulus: After habituation, a new stimulus is introduced to the infants. This second stimulus is different from the first one, allowing researchers to assess the infants' ability to discriminate between the two stimuli.
3. Dishabituation: If infants can discriminate between the two stimuli, they will show an increased response (e.g., looking time or attention) to the second stimulus compared to their response to the habituated stimulus. This increased response is known as dishabituation and indicates that the infants are capable of differentiating between the two stimuli.
To know more about Habituation
brainly.com/question/15155736
#SPJ11
When/why matter changes its phase?
Answers
In a prokaryotic cell, all of the following are functions of either fimbriae or pili EXCEPT __________.a. transfer of DNAb. protection from phagocytosisc. formation of biofilmsd. attachment to a surface
Answers
Option b is Correct. Except for phagocytosis defense, both fimbriae and pili serve the same tasks in bacterial cells.
Prokaryotic cells have small, hair-like features called pili on their surface. They can play a part in mobility, but more frequently they are involved in adhesion to surfaces, which promotes infection and is a crucial virulence trait. The oligomeric protein known as pilin makes up the extensions of the cytoplasmic membrane known as fimbriae and pili.
They are flagella-like extensions found on the bacterial cell wall that resemble hairs. They play a role in bacterial movement, surface adhesion, and conjugation. Prokaryotic cells have a specific sort of appendage called a fimbria (plural: fimbriae). Prokaryotes have protrusions that resemble hairs that help them adhere to surfaces in their environment.
Learn more about prokaryotic cell Visit: brainly.com/question/14771066
#SPJ4
what causes a population to reach is carrying capacity
Answers
Answer:
Good well fare taxes and pricing
Explanation: not only that but benefits with health and cost for homes and the environment
IN YOUR OWN WORDS, what are the definitions for these words?
1. Gamete:
2. Zygote:
3.Interkinesis (include when it happens):
4. Kinetochore:
5. Haploid:
Answers
Gamete;
When the zygote resulting from fertilization of an ovum (female sex cell) continues to grow, producing new cells by mitosis. This will continue until it reaches sexual maturity and then produce new cells by meiosis. These new cells, the germ cells gives rise to sex cells or the gametes.
Zygote;
The result of uniting the sex cells i.e the sperm and the egg is a zygote
Interkinesis;
Or interphase II is a period of rest that cells of some species enter during meiosis between meiosis I and meiosis II. During interkinesis, the single spindle of the first meiotic division disassembles and the the micro tubules reassemble, into two new spindle for the second meiotic division.
Kinetochore;
This is the protein complex assembled on the centromeric region of DNA. It provides the major attachment point for the spindle microtubles during mitotic or meiotic division to pull the chromosomes apart.
Haploid;
This is the quality of a cell or organism having a single set of chromosomes. Organisms that produce asexually are haploid organisms
Accepted Value (°C) Measurement in Lab (°C) seawater 100.7 101.8 mercury 359.6 370.0 jet fuel 163.0 165.9 ethanol (alcohol) 78.4 81.8 Which measurement has the highest percent error? seawater mercury jet fuel ethanol
Answers
Answer: ethanol
Explanation:
Explain why it makes sense that sperm and egg cells have 1/2 the amount of chromosomes than a normal cell that is not involved in reproduction.
Answers
Answer:
The sperm and egg have 1/2 the amount because when the sperm fertilizes the egg, it equals out to the regular amount of chromosomes. That is why we have half chromosomes from our dad and half chromosomes from our mom, equaling out to normal amount of chromosomes.
Answer:
It makes sense to me that that Sperm and Egg cels have only half the amount of chromosomes than a normal cell because, you get equal chromosomes from when they come together. so you get equal amount of traits from both sides.
Explanation:
it might be a bad explanation but i hope it helps
True of false Mendel realized that when he crossed two pea plants that were the offspring of a pea plant with yellow peas and a pea plant with green peas 50% of the offspring had yellow peas and 50% of the offspring had greem peas.
Answers
Answer:
False
Explanation:
Mendel realized that the F2 had a phenotypic ratio 3:1, meaning 3/4 = 75% were yellow and 1/4 = 25% green.
Mendel observed that the F1 expressed only one of the alternative variants (in this case, only yellow seeds appeared), while the other variant (green) disappeared. Mendel named dominant the expressed variant. Mendel allowed auto pollination and observed that in the second generation, F2, the other disappeared variant reappeared. Both alternative variants were present in the F2. Mendel named recessive the second alternative variant.
Mendel thought that hereditary traits determined by discrete factors were the possible explication for these phenotypes. These factors should have been present in the F1 in pairs. One of them came from one parental plant, and the other factor came from the other plant. These factors then separated again when sex cells were produced, giving two types of gametes, each with only one factor.
Mendel concluded that each individual (plant) has a pair of factors (alleles), one for each trait (yellow and green) and that the pair separates (segregates) during the formation of the gametes. This conclusion is known as the segregation principle (First Mendels´ Low).
Posture is maintained mainly by ______ fibers because they fatigue _________.
Question 1 options:
1)slow-twitch; slowly
2)white; slowly
3)type II; rapidly
4)fast-twitch; rapidly
5)fast glycolytic; slowly
Answers
Slow-twitch fibers are essential for maintaining posture since they lose strength gradually.
What are the types of fibers?Slow-twitch muscles, sometimes referred to as type I muscles, are designed specifically for endurance exercises and are able to sustain contractions for extended periods of time. They have a lot of mitochondria and myoglobin, which enables them to produce energy aerobically and use oxygen effectively. They are therefore well adapted for tasks like maintaining posture, which demands long-lasting, low-intensity contractions.Fast-twitch fibers, sometimes referred to as type II fibers, on the other hand, are designed for brief bursts of intensive exercises, like sprinting or weightlifting. These fibers can produce a lot of force but get tired easily, making them less suitable for exercises that call for prolonged contractions.learn more about Posture here
https://brainly.com/question/30704304
#SPJ1
magnesium’s major functions in the body include all of the following except: group of answer choices blood clotting. being a cofactor for many enzymes. stabilizing atp. maintenance of bones.
Answers
Magnesium’s major functions in the body include all of the following except blood clotting. Magnesium plays a vital role in many biological processes in the body.
It is essential for maintaining strong bones and healthy muscle function, as well as for the transmission of nerve impulses, maintaining a healthy immune system, and regulating heart rhythm.Magnesium is also a cofactor for many enzymes and plays a crucial role in stabilizing ATP, the energy currency of the body. However, magnesium does not play a role in blood clotting. Blood clotting is a complex process involving different proteins and platelets.
learn more about body
https://brainly.com/question/30621931
#SPJ11
What happens to the O2 and CO2 levels when one sprig of Elodea and one snail are put into a test tube with the lights on after 24 hours?
A: Drastic changes with oxygen increasing and carbon dioxide decreasing.
B: Drastic changes with carbon dioxide increasing and oxygen decreasing.
C: Oxygen and carbon dioxide vary slightly, but stay around their original levels.
D: There is no oxygen left in the test tube.
Answers
Elodea is a type of aquatic plant that performs photosynthesis, which is the process by which plants use sunlight, water, and carbon dioxide to produce oxygen and glucose. During photosynthesis, the plant absorbs carbon dioxide from the air and releases oxygen.
Snails, on the other hand, breathe using a process called respiration, which is the opposite of photosynthesis. During respiration, the snail takes in oxygen and releases carbon dioxide.
When you put a sprig of Elodea and a snail in a test tube with the lights on, the Elodea will perform photosynthesis and the snail will perform respiration. As a result, the carbon dioxide levels in the test tube will increase and the oxygen levels will decrease. The changes may be drastic, depending on the size of the test tube, the amount of Elodea and snail, and the intensity of the light.
A biodiversity hotspot is a region that has lost at least
Answers
Answer:
diversity throughout all living organisms
What is the molecular formula for methyl alcohol?
Answers
Answer:
CH3OH
Explanation:
Consider this image of a gazelle.
Which of the gazelle’s naturally selected traits is shown in this picture to be an evolutionary advantage?
Answers
Answer: I can think of one naturally selected trait in gazelle that is of great evolutionary advantage and can we shown on a picture well -its fur, which blends in the environment well (it has a similar colour). this trait would protect the gazelles from predators.
Answer:
B
Explanation:
its on edge
Identify whether the statement applies to autotrophs heaterotrophs or both autotrophsand heterotrophs
Answers
Answer:
Explanation:
i got it right on my test
The saguaro cactus provides food for the white-winged dove through its large fruit. The bird consumes the fruit, ingests the seeds, and then deposits the seeds in a new location.
Answers
Answer:
The answer is mutualism!!!
Explanation:
Just clicked it and it was correct.
The relationship between the saguaro cactus and the white-winged dove is an example of mutualism.
What is mutualism?The saguaro cactus provides a food source in the form of its large fruit, which the white-winged dove feeds on, while the bird helps to disperse the cactus seeds to new locations through its consumption of the fruit and subsequent depositing of the seeds.
This mutualistic relationship is important for both species and contributes to the ecological health and diversity of their shared habitat. The saguaro cactus relies on the white-winged dove and other animals to disperse its seeds, as it cannot move on its own. The bird benefits from the nutritious food source provided by the cactus fruit and is able to survive and reproduce more successfully as a result. Overall, this mutualistic interaction is an important part of the desert ecosystem where these species are found.
Learn more about mutualism, here:
https://brainly.com/question/13800737
#SPJ6
According to the Hertzsprung-Russell Diagram what is the luminosity of the sun
a) 1
b) 2
c) 3
d)4
Answers
The Sun, which has a luminosity of 1 and a temperature of around 5,400 Kelvin, is located on the main sequence. The correct option is A.
How do you read an H-R diagram?So, the H-R diagram may be used to compare the Sun to any other star on the diagram. Outside of the main sequence, there are two distinct groups of stars that are interesting in and of themselves. Many very brilliant but relatively cold stars may be seen in the diagram's upper right corner.
A Hertzsprung Russell H-R diagram displays what?Astronomical graph in which the actual magnitudes (intrinsic brightness) of stars are plotted against distinct spectral types is known as a Hertzsprung-Russell diagram, or H-R diagram (temperatures).
To know more about Hertzsprung-Russell Diagram visit:
https://brainly.com/question/12484470
#SPJ4
A ______ is often at the opposite side of a plate of a mid ocean ridge
Answers
Answer:
it's A for sure
Explanation:
took the test
The tropical rainforest is the most coplex and species rich biome in the world but it is soils are nutrient poor . What is the reason for this
Answers
Answer:
because nutrients cannot be stored in the soil for a long time
Explanation:
In rainforests, high temperatures and humid conditions lead to the rapid decomposition of the organic matter in the soils, which releases nutrients rapidly. However, rainforest soils are poor in nutrients due to rains that wash dead organic material from them. Moreover, rainwater is filtered naturally into the ground, thereby extracting (leaching) nutrients off the surface.
cerebrospinal fluid is formed by capillary knots called
Answers
Answer:
choroid plexuses
Explanation:
Cerebrospinal fluid is formed by capillary knots called choroid plexuses, which hang into the ventricles of the brain.
Look at the table for energy used in activities. Ella does 2 hours of dancing a week, plays football for 1 hour a week, and goes swimming for 0.5 hours a week. She has a mass of 55kg. How much extra energy will she need a week in order to do these activities?
Answers
Ella requires 3905 kJ of energy to do 2 hours of dancing a week, 1 hour of football a week, and 0.5 hours of swimming a week.
Ella, with a mass of 55 kg, does a series of activities in a week.
Let's calculate how much energy she needs for each activity in total.
DancingEllas does 2 hours of dancing a week, which consumes 18 kJ/kg.h.
2 h × 55 kg × 18 kJ/kg.h = 1980 kJ
FootballElla plays 1 hour of football a week, which consumes 25 kJ/kg.h.
1 h × 55 kg × 25 kJ/kg.h = 1375 kJ
SwimmingElla goes swimming for 0.5 hours a week, which consumes 20 kJ/kg.h.
0.5 h × 55 kg × 20 kJ/kg.h = 550 kJ
Total energy expenditureE = 1980 kJ + 1375 kJ + 550 kJ = 3905 kJ
Ella requires 3905 kJ of energy to do 2 hours of dancing a week, 1 hour of football a week, and 0.5 hours of swimming a week.
Learn more about energy expenditure here: https://brainly.com/question/4127266
cadherins share a property with the protein calmodulin, which is involved in transmembrane transport, as discussed in chapter 11. in either case, both of these proteins:
Answers
Both cadherins and calmodulin are proteins involved in cellular signaling and regulation. They share a property of being transmembrane proteins, meaning they are embedded in the cell membrane and play a role in transport and communication between cells.
Cadherins are important for cell adhesion and maintaining tissue structure, while calmodulin is involved in calcium signaling and regulating the activity of various enzymes and ion channels. Overall, both proteins play crucial roles in maintaining cellular function and communication.
dependent cell-cell adhesion is mediated by the cadherin family of transmembrane proteins. Adhesion is achieved by homophilic interaction of the extracellular domains of cadherins on adjacent cells, with the cytoplasmic regions serving to couple the complex to the cytoskeleton. IQGAP1, a novel RasGAP-related protein that interacts with the cytoskeleton, binds to actin, members of the Rho family, and E-cadherin.
Calmodulin binds to IQGAP1 and regulates its association with Cdc42 and actin. Here we demonstrate competition between calmodulin and E-cadherin for binding to IQGAP1 both in vitro and in a normal cellular milieu. Immunocytochemical analysis in MCF-7 (E-cadherin positive) and MDA-MB-231 (E-cadherin negative) epithelial cells revealed that E-cadherin is required for accumulation of IQGAP1 at cell-cell junctions.
To know more cadherins and calmodulin click this link-
brainly.in/question/42188497
#SPJ11
What is true of table salt?
D.It has simple molecules.
C.It has only one element.
B.It is an ionic compound.
A.It has radicals.
Answers
Answer:
c because it is
Differentiate between subsistence farming and commercial farming .
only 2 points:)
Answers
Answer:
Difference between subsistence farming and commercial farming
Explanation:
Subsistence farming is done basically to feed the farmers family itself. It has little or no profit.
Commercial farming is done with the intent of profit, where cash crops are sown.
hey could someone help me with this immediately! i’ll mark brainiest if u don’t guess or leave a link!!
Answers
Answer:
the fourth one. hope this helps:)
Explanation:
Answer:
Plant 4
Explanation:
Joshua had been giving his plants 1/2 cup two times a week. This is the control group, because it remains unchanged.
For a given species, uniform thickness of a cell membrane requires uniform chain length of its fatty acids. How do you think chain length may be regulated
Answers
For a given species, uniform thickness of a cell membrane requires uniform chain length of its fatty acids. The chain length may be regulated through the process of fatty acid synthesis
The length of the chain can be controlled by the enzymes involved in fatty acid synthesis, which can either elongate or shorten the fatty acid chain. Additionally, the availability of precursors for fatty acid synthesis can also play a role in regulating chain length. For example, if there is an abundance of short-chain fatty acid precursors, the resulting fatty acids may be shorter in length. The regulation of chain length is important for maintaining a uniform thickness of the cell membrane, which is necessary for proper cell function.
Deviations from uniformity can result in changes in membrane fluidity and permeability, which can ultimately lead to cellular dysfunction. Overall, the regulation of chain length in fatty acids is an important aspect of maintaining cellular homeostasis through the process of fatty acid synthesis
To learn more about enzymes here:
https://brainly.com/question/29774898
#SPJ11
Several different amino acids join together to form a protein. How do any two types of amino acids differ from one another?
a. the number of amino groups in the molecule b. the number of carboxyl groups in the molecule C. the chemical structure of the R group in the molecule d. the number of chemical bonds formed by the central carbon atom
Answers
A widow's peak hairline (W) is dominant to straight hairline (w). Cross a heterozygous widow's
peak hairline person with a straight hairline person. What are the genotypic and phenotypic
ratios of their offspring
Answers
Answer:
Use a punnett square! I'll attach an example.
Genotype - Genetic constitution of dominant and recessive traits inherited from parents. BB, Bb, & bb can be used to represent these.
Phenotype - Observable. If a dominant trait is present, it shows as the phenotype. For example, brown hair is dominant to blonde hair. A person with a BB or Bb genotype will have brown hair, but a person with a bb genotype will have blonde hair.
Statistically, they have a 50% chance of a child with a widow's peak, and a 50% chance of a child with a straight hairline.
The genotypic and phenotypic ratios of their offspring are 50% and 50% ratio of the child.
What are the heterozygous ?
The heterozygous are the condition where you have inherited different forms of a particular gene from each of your biological parents. By different shapes we generally mean that there are different parts of the gene where the sequence is different.
Genotype - Genetic charter of dominant and recessive developments inherited from parents. BB, Bb, & bb may be used to symbolize these.Phenotype - Observable. If a dominant trait is present, it indicates because the phenotype. For example, brown hair is dominant to blonde hair. A individual with a BB or Bb genotype may have brown hair, however someone with a bb genotype may have blonde hair.Statistically, they've a 50% risk of a toddler with a widow's peak, and a 50% risk of a toddler with a instantly hairline.
Read more about the offspring:
https://brainly.com/question/16676548
#SPJ2
How are CO2 and O2 used or produced by orcas?
Answers
Answer:
basically every living thing breathes oxygen O2 and exhales carbon dioxide CO2
Explanation:
So orcas use O2 and produce then CO2.