Part 1 (1 point) w See Periodic Table You are an undergraduate research student working in a laboratory that studies breast cancer cells. You are asked by the lab manager to determine whether Protein X is overexpressed in breast cancer cells. Which of the following would be the most appropriate strategy for determining this? Choose one: O Perform SDS-PAGE and Western blot to demonstrate relative abundance of Protein X in normal cells and breast cancer cells. O Use affinity chromatography to separate the normal cell Protein X from the cancer cell Protein X. O Combine the normal and cancer cell samples and perform isoelectric focusing to determine relative abundance of Protein X. O Determine an A280 value for both normal cells and breast cancer cells to determine total protein concentrations.

Answers

Answer 1

The most appropriate strategy for determining whether Protein X is overexpressed in breast cancer cells would be to perform SDS-PAGE and Western blotting to demonstrate the relative abundance of Protein X in normal cells and breast cancer cells.

Protein X is a type of protein. A protein is a complex, high-molecular-weight organic compound made up of amino acids, the building blocks of life. These macromolecules are responsible for a wide range of biological processes in living organisms. When analyzing the overexpression of Protein X in breast cancer cells, SDS-PAGE and Western blotting are the most appropriate methods.

SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a technique used to isolate and analyze proteins. Proteins are separated in a gel matrix by molecular weight using an electric field.

After the proteins have been separated, a Western blot is used to detect a specific protein in the separated mixture. It can be used to determine whether protein X is overexpressed in breast cancer cells.

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Related Questions

Help please!! I'll name you brainliest!!

Help please!! I'll name you brainliest!!

Answers

b the uv one :)))))))))

Each of the following regions/markings is associated with the occipital bone except the

A) superior and inferior nuchal lines.

B) foramen magnum.

C) hypoglossal canals.

D) glabella.

E) external occipital crest.

Answers

Each of the following regions/markings is associated with the occipital bone except the glabella. option D is correct.

The trapezoid-shaped bone called the occipital bone is where the back portion of the brain is located. The occipital bone, one of seven bones that make up your skull, is situated near the lower-back of the cranium.

The occipital bone's crucial function is that it  protects our brain. It safeguards the area of the brain responsible for processing vision. Additionally, it serves as the link between the brain and the spine.

The occipital bones of an individual's skull will fuse together as they get older. Between the ages of 18 and 25, the centre of your skull's sphenoid bone and occipital bone will fuse together. The top of your head's parietal and occipital bones will then fuse together sometime between the ages of 26 and 40.

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Which of the following do all organisms have in common?

Answers

Answer:

What Do All Organisms Have In Common? All living organisms share several key characteristics or functions: order, sensitivity or response to the environment, reproduction, growth and development, regulation, homeostasis, and energy processing. When viewed together, these characteristics serve to define life.

Explanation:

12 The second softest mineral according to the Mohs scale of hardness is the
A Tale
B. Apatite
C Calcite
D. Gypsum​

Answers

The Mohs scale minerals from softest to hardest:

1. Talc (softest)

2. Gypsum

3. Calcite

4. Fluorite

5. Apatite

6. Feldspar

7. Quartz

8. Topaz

9. Corundum

10. Diamond (hardest)



If I am wrong please tell me
the answer would be talic so it would have to be A

1. Which of the following is an example of sensitization:
You blink each time your eye doctor gives you a puff of air in your eyes.
A dog was hit by his previous owner, and now the dog cowers each time his new owner reaches out to pet him.
You no longer feel your shirt touching your skin.
You're walking on the sidewalk and a bicyclist almost hits you, so you jump out of the way.

Answers

The example of sensitization from the given alternatives is that 'A dog was hit by his previous owner, and now the dog cowers each time his new owner reaches out to pet him.'

Sensitization refers to the process in which an organism becomes more sensitive to stimuli, both good and bad. It happens when the body's nervous system becomes oversensitized to a particular stimulus that it begins to respond even to weaker or non-threatening stimuli.

The example given in the first option refers to a simple reflex action that occurs in response to the puff of air that is blown into the eyes during the eye examination. The third option is about sensory adaptation, which occurs when sensory receptors are exposed to stimuli for an extended period, resulting in a reduction in sensitivity. The fourth option refers to the fight or flight response that occurs as a result of a threatening stimulus, in which an individual's body prepares to confront or flee from danger.

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lood moves into and then out of a heart chamber because select one: a. it moves along its pressure gradient, and that gradient depends on contraction and relaxation during the cardiac cycle. b. it is under constant pressure, but its movement is dictated by the control of valve openings and closures. c. all of the choices are correct. d. the veins and arteries constrict and dilate to propel and attract blood.

Answers

Blood moves into and then out of a heart chamber because a. it moves along its pressure gradient, and that gradient depends on contraction and relaxation during the cardiac cycle.

In general , the  pressure gradient usually mediate blood out of the left atrium also produces a  rapid early diastolic flow that soon propagates to the apex. As the diastolic intraventricular pressure gradient helps to pull out the blood to the apex, this is known as the left ventricular suction.

The blood moves in a cardiac cycle through ventricles and atria together by the process of relaxation and expansion also blood flows to the heart during ventricular and atrial diastole. when ventricles are relaxed and expanded the  atrial contraction or systole forces blood under pressure into ventricles during ventricular diastole.

Hence, A is the correct option

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the auditory sensory axons of the___branch of cranial nerve number__terminate in the___cochlear nucleus within the brainstem.

Answers

The cochlear nucleus in the brainstem is the location where auditory sensory axons of a cochlear branch for cranial nerve no.8 terminate.

An sensory axon is what?

The axons of some sensory neurons, like those responsible for touch and temperature, are known as afferent nerve fibers, and the motor neuron travels down them from the medulla oblongata to the periphery as well as along an branch of identical axon from the medulla oblongata to the spinal cord.

Where can you find sensory axons?

Particularly, sensory axons that emerge from epidermal (or muscle) and send information to the nervous system will behave differently than motor axons that control muscle muscle and make it contract. A nerve cell, or nerve cell, has a slender fiber called an axon that it projects from.

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How can mutation lead to diversity and variation?


30 points >:)

Answers

Answer:

The flow of individuals in and out of a population introduces new alleles and increases genetic variation within that population. Mutations are changes to an organism's DNA that create diversity within a population by introducing new alleles

Mark me as the brainlist

PLZ I'M NOT TRYING YO GOT TO SUMMER SCHOOL I WILL BRAINLIST YOU
true or false All arthropods belong to the Class Insecta

true or false All rodents belong to the phylum Chordata.

true or false All amphibians belong to the class reptilia.

true or false All primates are mammals.

true or false A lion belongs to the genus Felis.

true or false All mammals are primates.

true or false All Insects and lobsters are arthropods.​​

PLZ I'M NOT TRYING YO GOT TO SUMMER SCHOOL I WILL BRAINLIST YOUtrue or false All arthropods belong to

Answers

Answer:

1.)true

2.)true

3.)true

4.)true

5.)true

6.)false

7.)false

Explanation:

Answer:

False: All arthropods belong to the Class Insecta

True: All rodents belong to the phylum Chordata.

False: All amphibians belong to the class reptilia.

True: All primates are mammals.

False: A lion belongs to the genus Felis.

False: All mammals are primates.

True: All Insects and lobsters are arthropods.​​

Hope this helps :)

compare sources and sinks of carbon dioxide

Answers

Answer:

A carbon sink is anything that absorbs more carbon from the atmosphere than it releases – for example, plants, the ocean, and soil. In contrast, a carbon source is anything that releases more carbon into the atmosphere than it absorbs – for example, the burning of fossil fuels or volcanic eruptions.

Harry suffers from cystic fibrosis and has severe breathing difficulties. His problems result from

Answers

Harry's cystic fibrosis, which is a genetic disorder that affects the production of mucus in his body.

The excess mucus in his lungs and airways makes it difficult for him to breathe and can lead to frequent infections and inflammation. Harry's respiratory problems can also cause him to experience fatigue, chest pain, and difficulty exercising or engaging in physical activity. To manage his condition, Harry may require daily medications, physical therapy, and other treatments to improve his lung function and quality of life. Harry's cystic fibrosis, which is a genetic disorder that affects the production of mucus in his body.  To manage his condition, Harry may require daily medications, physical therapy, and other treatments to improve his lung function and quality of life.

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True or false the nigrosin used for indirect staining is an acidic stain which binds to negative parts of the cell.

Answers

It is a false statement that he nigrosine used for indirect staining is an acidic stain which binds to the negative parts of the cell.

What is nigrosine?

The dye nigrosine is a type of black dye that is formed when by heating a mixture of nitrobenzene, aniline, and hydrochloric acid in the presence of copper or iron.

This dye is known to be useful in staining of the cells of living organisms. It is a false statement that he nigrosine used for indirect staining is an acidic stain which binds to the negative parts of the cell.

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please helppp asap, have a good rest of your day !

please helppp asap, have a good rest of your day !

Answers

Answer:

hh × Hh

The results showed that roughly 50% of the mice are brown and roughly 50% of the mice are black. So the genotypes of the mice must be hh and Hh. (punnet squares are in the image attached)

Explanation:

hh - brown

Hh - black

please helppp asap, have a good rest of your day !

What is the primary source of protein in your diet?

Answers

Answer:

animal source and plant sorce

Explanation:

a.eggs,meat

b.legumes

Eggs, beans, tofu, meat if you eat it

PLEASE HELP! COLOR BY NUMBER! CORRECT MY ANSWER IF THEY ARE WRONG! THANK YOU!​

PLEASE HELP! COLOR BY NUMBER! CORRECT MY ANSWER IF THEY ARE WRONG! THANK YOU!

Answers

A BBee genotype Labrador will be black in color.

Epistatic genes

Epistatic genes are genes that can affect the expression of other genes, even those on different chromosomes, by either enhancing or suppressing their expression. They can influence the phenotype of an individual by masking or modifying the effects of other genes.

A Labrador with BBee genotype will be black in color.

This is because BB denotes the dominant black color, and ee denotes the recessive yellow color. Since yellow is recessive epistatic, it will mask both brown and black colors. Therefore, the dog will express the dominant black color even though it carries the recessive yellow allele.

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Explain in your own words what a dichotomous key is:

Answers

Answer:to know which animals belong to which classification or which group they are. And to know they features like how many legs and how many fingers like this. Like the animal what makes it special from others animals

Explanation: hope this help

Classify the hypothesis about the dwarf planet Pluto as falsifiable or non-falsifiable

Answers

The hypothesis about the dwarf planet Pluto is falsifiable. This means that the hypothesis can be tested through experiments or observations to determine whether it is true or false.

The hypothesis that Pluto is made of ice, can be tested by observing the planet's surface and analyzing its composition. If the results show that Pluto is not made of ice, then the hypothesis is proven false.
On the other hand, a non-falsifiable hypothesis is one that cannot be tested or proven false. For example, if the hypothesis is that Pluto is the most beautiful planet in the solar system, this cannot be tested or proven false because beauty is a subjective concept and cannot be measured objectively.
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What are all the parts of a plant?

Answers

Answer:

Stem.

Estate.

Leaves.

Flower.

Fruit.

Answer:

What are all the parts of a plant?

SeedsLeavesStemsRootsFruitsFlower petals

Explanation:

You're welcome.

Identify examples of adaptation in organisms
(Do not use the artic fox example)

Answers

Answer:

Frog adaptations

- thin skin which allows them to breathe through their skin

- strong hind legs and feet allow them to jump long distances

- small, thin body structure which allows them to be excellent swimmers

- bulging eyes allow them to be better hunters

Answer:

Camouflage, mimicry, and animals' body parts and coverings are physical adaptations. The way in which an animal behaves is an adaptation, too—a behavioral adaptation . Behaviors that animals are born knowing how to do are instincts . A lion preying upon a zebra and a bird building a nest are examples of instincts.

Explanation:

HELP ASAP
if a molecule passes through a protein but goes down a gradient it is called
a. facilitated diffusion
b. exocytosis
c. active transport
d. endocytosis​

Answers

Answer: I think it would be A. Facilitated diffusion

Explanation: I think it would be a cause some molecules can move down their concentration gradients by crossing the lipid portion of the membrane directly while others have to pass through membrane proteins in a process called facilitated diffusion

If im wrong srry!

Hope This helped!

Answer:

The answer is facilitated diffusion

Explanation:

in cells,some molecules can move down their concentration gradients by crossing the lipid portion of the membrane directly, while others must pass through membrane proteins in a process called facilitated diffusion.

The movement of substances from the bloodstream into the tubular nephron is referred to as:.

Answers

Answer:

tubular reabsorption I think

A brown-eyed man whose mother was blue-eyed married a brown-eyed woman whose
father had blue eyes. Assume brown to be dominant over blue. What is the probability
that the couple will have a blue-eyed child?

Answers

The probability will be 0.25 or 25%

Bacterial smears are fixed with
a. water
b. bunsen burner
c. none of the above
d. heat

Answers

Answer:

the answer is water

Explanation:

because it is

select all the statements that define evaporation (vaporization) and condensation.

Answers

The incorrect statement concerning evaporation is: "Increasing the surface area of the liquid decreases the rate of evaporation." Option A is correct.

In reality, increasing the surface area of the liquid actually increases the rate of evaporation. This is because evaporation occurs at the surface of the liquid, and a larger surface area provides more space for molecules to escape into the vapor phase. Evaporation is a process by which a liquid changes into a gas or vapor state.

It occurs when molecules near the surface of a liquid gain enough energy to overcome intermolecular forces and escape into the surrounding space as vapor. Increasing the temperature of the liquid is one of the factors that can significantly affect the rate of evaporation. As the temperature rises, the average kinetic energy of the molecules increases, resulting in more molecules having sufficient energy to transition from the liquid to the gas phase, option A is correct.

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The question is inappropriate; the complete question is:

Which of the following statements concerning evaporation is incorrect?

A. Increasing the surface area of the liquid decreases the rate of evaporation.

B. Increasing the temperature of the liquid increases the rate of evaporation.

C. Evaporation causes the liquid temperature to decrease.

D. Molecules with energies considerably above average are those that escape from the liquid.

Describe how temperature and salinity affect the solubility of gases in the surface ocean

Answers

Answer:

The solubility of gases such as oxygen and nitrogen depends upon the ocean water's temperature and salinity. As the temperature of the ocean water increases, the solubility of these gases decreases. The solubility of carbon dioxide is greater than the other gases because of its ability to react with ocean water.

How do plant in animal responses to stimulate increase their fitness for specific environments? (I’m on edge).

Answers

Plants and animals have evolved a variety of responses to stimuli that increase their fitness for specific environments. These responses can be broken down into two categories: physiological and behavioral.


Both physiological and behavioral responses can increase an organism's fitness by allowing it to better survive and reproduce in specific environments. For example, if a plant is able to grow more quickly in high light conditions, it may be able to outcompete other plants for resources and reproduce more successfully. Similarly, if an animal is able to find food more easily by migrating, it may be better able to survive and reproduce.


In summary, plants and animals have evolved a variety of responses to stimuli that increase their fitness for specific environments. These responses can be physiological or behavioral and allow organisms to better cope with changes in their environment, which ultimately increases their chances of survival and reproduction.

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While on a hike you find Quant plants with blue flowers and Quant plants with white flowers surrounding a secluded lake. You do the unthinkable and take four Quant plants back to the lab, none of which have flowers yet. Luckily when flowers emerge you notice that you have two plants with blue flowers and two white flower bearing plants. You allow these plants to self with the following results and then complete two crosses. 3. Progeny Number White Blue 89 Pollination Blue plant #1 (self) white plant #1 (self) Blue plant #2 (self) White plant #2 (self) 38 18 59 54 | Blue #1 x White #2 Blue #2 x white #1 62 38 How many genes are involved in Quant flower petal pigmentation? Describe type of dominance is exhibited by the genels) and alleles? What are the genotypes of the four plants you brought back to the lab? what genotypic and phenotypic ratios would you expect if you crossed Blue #1 with white #1?

Answers

There are two genes involved in Quant flower petal pigmentation, and the type of dominance exhibited is incomplete dominance and the expected phenotypic ratio will be 1:1.

What is the phenotypic ratio?

The genotypes of the four plants brought back to the lab are two blue homozygous plants (BB), one white homozygous plant (ww), and one heterozygous plant (Bw). If we crossed Blue #1 with White #1, it is expected to have a genotypic ratio of 1 Blue homozygous (BB) to 1 White homozygous (ww) and a phenotypic ratio of 1 Blue flower (Bw) to 1 White flower (Bw).

The color of the flower in Quant plants can be determined by two genes involved in flower pigmentation. Each gene has two alleles, and each of the alleles shows a co-dominant expression. The genotypes of the four plants that were brought to the lab are not known. A total of four genotypes is possible. These are; White - homozygous recessive (ww), White - heterozygous (Ww), Blue - heterozygous (Bw), and Blue - homozygous dominant (BB).

In the cross of Blue #1 with white #1, the genotypic and phenotypic ratios that would be expected are as follows; Phenotypic ratio: 1: 1, Genotypic ratio: 1 WW: 1 Bw.

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good movie recommends? My teacher said I get to choose the movie for today

Answers

Answer:

titanic you teacher will think you are very mature or you gold do something like hobbs and shaw really good or you could watch  endgame lol

Explanation:

Answer:

Explanation:

Instant family or playing with fire are both Carey amazing movies

10. Which of the following statements does NOT correctly describe a DNA molecule?
A. The two strands of DNA run in opposite directions.
B. Each strand of DNA has a 5' and a 3' end.
C. Both strands are curved in a spiral formation.
D. The nucleotide strand is complementary to the nitrogenous strand.

Answers

The sugar and phosphate group joins together to make the backbone of the DNA molecule and the nitrogenous bases aligned perpendicular to this backbone.

What is DNA and what does it do?The information molecule is DNA. It provides the knowledge required to make proteins, another type of important molecule. The 46 major chromosome sets that make it up every one of your cells have these instructions. The numerous little DNA pieces that make up each chromosome are known as genes.DNA, also known as deoxyribonucleic acid, appears to be a molecule that houses the genetic material required for a creature to grow and function. The double helical, which is composed of two linked threads that loop around each other to resemble a twisted ladder, is the shape of DNA.The base-pairing guidelines are followed by the nucleotides pairs in double-stranded DNA: A with T, G with C, etc. A double-stranded molecule with complementary, antiparallel strands makes up DNA.DNA is a nucleic acid made up of nucleotides which joins to each other to form two strands of DNA. Each strand is made up of nucleotide composed of a 5C deoxy-ribose sugar, a phosphate group and nitrogenous base.

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What are the two unique characteristics shared by all birds

Answers

Explanation:

All birds usually have feathers and wings

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