how long do you need to wait after creating a bacterial lawn before you can put antibiotic disks onto the plate?

Answers

Answer 1

You need to wait 15 minutes after creating a bacterial lawn before you can put antibiotic disks onto the plate.

How is lawn bacteria created in lab?

Bacterial lawns are produced manually by spreading evenly, a high amount of bacteria onto an agar plate using sterile cotton swabs. An automated machine can be also be used, alternatively, such as a spiral plater where the plate is rotated and the sample is spread evenly using an automated dispenser.

If the ethanol catches fire, then the lid can be placed across the top to remove oxygen supply to the fire. While taking disc diffusion testing, it is important to get the antibiotic discs on the plates within 15 min of creating the bacterial lawn.

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Related Questions

??help please
I don't understand what it is
i need it for now ​

??help pleaseI don't understand what it is i need it for now

Answers

It’s releasing heat into the atmosphere because the oceans absorb solar and thermal radiation and release that heat into the atmosphere

Determine the amino acid chain that would result from the sequence AUG-CCC-GGA-UUA-UAG.

Answers

Answer:

methionine-proline-glycine-leucine-stop

Explanation:

Using an amino acids chart, you can line up the 3 bases in each section to find the protein chain that corresponds. The stop at the end marks the end of protein synthesis, which ends the sequence.

The compound comprising carboxylic and amino groups is called amino acids. They are vital for the formation of proteins in the cells.

Sequences are read in the three pairs of codons in the form of triplets to form a form an amino acids sequence.

The amino acid sequence that will be formed from the given sequence AUG-CCC-GGA-UUA-UAG will be:

methionine-proline-glycine-leucine-amber

AUG is the start codon which also codes for the amino acid methionine.

CCC codes for the amino acid proline and its one-letter code will be P.

GGA is glycine and is abbreviated as G.

UUA codes for leucine and is represented by U.

UAG is the amber or the stop codon which is responsible for terminating the sequence.

Therefore, Met-Pro- Gly- Leu- Amber is the amino acid sequence.

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PLEASE HELP!! give legit answer plssss
Key Questions and Terms Notes
What types of muscles are in the body?
What are muscles?
What's the difference between voluntary muscles and involuntary muscles?
What are the different types of muscle tissue?
How do skeletal muscles work?
What five functions does the skeleton perform?
The backbone is made up of 26 small bones called ___________.
What are the characteristics of bones?
What role do joints play?
What are the two types of joints?
What are ligaments?
What is the role of the nervous system?
Which parts of the body make up the central nervous system?
Name the two main categories of the nervous system.
How do the muscular, skeletal, and nervous systems work together to maintain homeostasis?
What do sensory neurons do?
What do motor neurons do?
Nervous System Video:
Word Bank: brain, instincts, interpreted, organs, reflexes, spinal cord, senses Notes
The central nervous system includes the _______ and _______.
Signals from _______ and the body's _______ are received by the brain and the spinal cord.
Once signals are processed and _______ by the central nervous system, a response is sent back to the body.
A central nervous system is also responsible for programming _______ and _______.
Paraplegic Will Walk Again with Exoskeleton at the World Cup Video:
Key Questions and Terms Notes
What animal did scientists study to create the brain–machine interface?
What does the exoskeleton use to power its movements?
What is the importance of this research?

Answers

Answer:

Answer B

Explanation:

Plato

What might be some of the economic and environmental tradeoffs of producing clothes from human-made polymers, such as nylon, and polyester versus natural polymers, such as cotton?

Answers

Answer:

Synthetic clothes are economically less expensive and lightweight compared to common traditional clothes; however, they pose difficulties for recycling

Explanation:

Polymers are compounds made of long repeating molecules, which can be classified according to their origin into natural and synthetic. Synthetic polymers include, among others, nylon (household products), polyvinyl chloride, also known as PVC (clothing), polypropylene (packaging), etc. As compared to traditional clothes made up of cotton, synthetic clothes offer significant advantages related to production costs, i.e., they can be produced in high-scale processes, and automated production is less expensive than traditional processes. However, synthetic polymers consist of long repeat chains of molecules that have very strong atomic bonds, which make difficult their degradation by decomposers (i.e., non-biodegradable in nature). The process of degradation of synthetic clothes can take up many years to break down these compounds. In addition, synthetic clothes are also pollutants because they are lost in the water when they are laundered, remaining for many years in water ecosystems.

The price of cotton is very high, because it is a natural fiber that must be cultivated and then harvested in certain months of the year, which in itself is a disadvantage because it interrupts the continued production of textile merchandise.

The above generates a very strong economic impact, in addition the environmental impacts are what most concern since to keep this crop in good condition, the use of pesticides, fertilizers and other minerals is constantly used that greatly pollute rivers and lakes, affecting Biodiversity. Cotton is the largest consumer of water among agricultural products, which also generates a great depletion of this valuable water resource.

For the above reasons, the use of artificial polymers emerged as a more economical and environmentally friendly alternative, since sports garments are cheaper and more resistant, they can also be kept longer, they are also more resistant to wrinkles, moths and mold.

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how can an understanding of mendelian genetics be used in genetic counseling?

Answers

The understanding of Mendelian genetics can be used in genetic counselling such that the counsellors can inform the couple about the odds or disease that may occur in their offspring. Or the history for a specific disease can be traced from the family tree.

Mendelian genetics refers to the laws of genetics established by Mendel. These are : the law of dominance, the lay of segregation and the law of independent assortment. All the laws describe how the traits are passed on from parents to their offspring.

Genetic counselling is the process of examining the genetic history of an individual. It includes the formation of genetic family tree or pedigree through which the heredity can be studied. It also helps in detecting the defects in future generations.

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7- If a starch-digesting enzyme were added to a sports gel that lists starch as an ingredient, which substance would increase in concentration?

(1) fat (2) amino acids (3) glucose (4) water

Answers

Answer:

Glucose

Explanation:

anyone know the correct choice to the question?

anyone know the correct choice to the question?

Answers

Answer:

person 1 in row 3's genotype AA or Aa

Explanation:

the trait is recessive (given) which means in order to have it, you must obtain both alleles. in the pedigree, shaded shapes indicate people who have the trait, but person 1 in row 3 is not shaded so their genotype must be AA (dominant) or Aa (dominant leading) and their phenotype would not express the trait, because the dominant allele will "take over"

If you move 50 meters in 10 seconds, what is your speed?

If you move 50 meters in 10 seconds, what is your speed?

Answers

Answer:

5 meter / second ..................

which of the following blood types is considered a "universal reciever" A B AB O

Answers

Answer:

o

Explanation:

13. A group of first grade students planted seeds in cups of soil. They placed some cups near the windows to get sunlight and others in a closet where no light was present. After a week, they measured the growth of the plants. What is the independent variable?

A. Time
B. The seed
C. The growth of the plant
D. The amount of light ​

Answers

I might be wrong but I think it’s A
amount of light is independent so its D

What is the highest period of a sound wave that can be detected well by the diaphragm on a stethoscope?

Answers

Regarding your question about the highest period of a sound wave that can be detected well by the diaphragm on a stethoscope, let me provide some information.

A stethoscope is a medical instrument used by healthcare professionals to listen to sounds produced by the body, such as heartbeats, lung sounds, and bowel sounds.

The diaphragm of a stethoscope is the circular, flat part that comes in contact with the patient's body. It is designed to pick up high-frequency sounds. When sound waves enter the diaphragm, they cause it to vibrate, and these vibrations are then transmitted through the stethoscope tube to the healthcare professional's ears.

The highest period of a sound wave refers to the frequency or pitch of the sound. Period is the inverse of frequency, so a higher period means a lower frequency. The diaphragm of a stethoscope is more sensitive to higher-frequency sounds, such as the higher-pitched heart murmurs or lung sounds.

However, there is a limit to the highest frequency that can be detected effectively by the diaphragm. This limit depends on various factors, including the design and quality of the stethoscope and the individual's hearing capabilities. Generally, the diaphragm of a standard stethoscope can detect sounds up to around 2,000 to 3,000 Hz, which corresponds to a period of approximately 0.33 to 0.5 milliseconds.

It's important to note that the diaphragm's ability to detect sound is not solely determined by the highest period but also by other factors such as the amplitude (volume) of the sound wave and the presence of background noise. Additionally, specialized stethoscopes, such as electronic or digital models, may have different frequency response ranges and may be capable of detecting higher frequencies.

In summary, the highest period of a sound wave that can be detected well by the diaphragm on a stethoscope typically falls within the range of 0.33 to 0.5 milliseconds, corresponding to frequencies up to around 2,000 to 3,000 Hz.

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what is similar and different about the three forelimbs? how does this information provide evidence of common ancestry?

Answers

The forelimbs refer to the arms of humans, the wings of a bat, and the fins of a whale. Despite their different functions, these forelimbs share some similarities that suggest they share a common ancestor.

Similarities:

They all have a similar bone structure, with the humerus (arm bones), two forearm bones (radius and ulna), carpal bones (wrist), hand bones (metacarpals), and fingers hand (phalanges). All of them have joints that allow movement in multiple directions.

Difference:

The human arm is adapted to grasp and manipulate objects with the opposite thumbs. The bat's wings are adapted for flight, with a thin membrane of skin extending between the elongated finger bones. The fins of whales, adapted for swimming, are flattened, broad.

The concept of homologous structure supports the idea of ​​a common ancestor, as it suggests that different species evolve from a common ancestor and inherit similar structures that have been modified to meet their needs. their specific needs.

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Which option is an example of something that can promote sustainable development?
Responses

(A.) having a leaky septic tank

(B.) clearing acres of land to build a home

(C.) using solar panels for heat

(D.) having a large yard

Answers

Answer:

(C.) using solar panels for heat

Explanation:

k 12 quiz

Which option is an example of something that can promote sustainable development?Responses(A.) having

Having a large yard is an example of something that can promote sustainable development. Thus, the correct option is D.

What is Sustainable development?

Sustainable development is an organizing principle for the meeting of the human development goals while it is also sustaining the ability of the natural systems to provide the natural resources and ecosystem services on which the human economies and society depends as a whole.

A yard is an open place which has fencing around it, with the grasses and small shrubs around as well. Having a large yard will not only meet the requirements of the human beings however it also be in favor of the environment as there will be no construction over that area due to the yard.

Therefore, the correct option is D.

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I NEED QUICK HELP!!!

Which kind of resource would an agrarian society need to manage particularly
carefully?
O A. Wildlife
O B. Fossil fuels
C. Soil
O D. Forests

Answers

Answer:

Soil

Explanation:

They are farming so in order to farm you need soil

use some knowledge and common sense

Make me brainliest

The answer to your question would be soil.

A student creates a study guide describing each of the five phases of a neuronal action potential. How should she fill in the blanks for the phase indicated above?A. A = Rapid diffusion into cell; B = Equal movement in and out of cell; C = Most positive value
B. A = Slow diffusion out of cell; B = No movement; C = Most positive value
C. A = Equal movement in and out of cell; B = No movement; C = Most negative value
D. A = Rapid diffusion out of cell; B = Equal movement in and out of cell; C = Most negative value

Answers

Action potentials go through four phases: depolarization, overshoot or peak phase, repolarization, and refractory period. The three phases of an action potential are depolarization, overshoot, and repolarization. Hence (a) is the correct option.

In relation to the action potential, the membrane potential has two additional states. First, there is hypopolarization, which comes before depolarization, and then there is hyperpolarization, which comes after repolarization. The three primary phases of a neural action potential are depolarization, repolarization, and hyperpolarization. The threshold voltage of the cell, or the membrane potential at which voltage-gated sodium channels (Nav) open to let an inflow of sodium ions occur, controls how depolarized the cell becomes initially.

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Which area of the backbone is referred to as your tailbone?
thoracic
sacrum
coccygeal
lumbar

Answers

Answer:

The coccygeal bone, at the base of the spine, is commonly referred to as the tailbone. So, the answer is the letter C.

Hope this helps!! :)

Explanation:

100 PTS——-Describe a specific detrimental human impact to a particular ecosystem and propose a solution to reduce the harmful effects over time. Explain how your proposed solution will work to minimize effects in that ecosystem.

Answers

Answer: human trash

Explanation:

Because we leave or trash and it decomposes into harmful air or water

Which of the following statements describes one of the technical reasons why gene therapy is problematic in humans? a. Most cells with an engineered gene do not produce gene product b. Transferred genes may not have appropriately controlled activity. c. Cells with transferred genes are unlikely to replicate. d. mRNA from transferred genes cannot be translated.

Answers

One of the technical reasons why gene therapy is problematic in humans is that transferred genes may not have appropriately controlled activity. The correct option is b.

This means that the engineered gene may not function as intended and may even cause unintended effects in the body. This is due to the complex nature of gene regulation and the difficulty in controlling gene expression once it is introduced into the body.

Other technical challenges in gene therapy include issues with delivery, ensuring that the correct cells are targeted, and avoiding immune reactions against the introduced gene.

Transferred genes may not have appropriately controlled activity. Gene therapy involves introducing exogenous genes into a patient's cells to correct a genetic defect or provide therapeutic benefits. However, controlling the activity of these transferred genes is crucial for ensuring their effectiveness and safety.

One challenge is achieving the desired level and timing of gene expression. The transferred genes may be under the control of a regulatory sequence that is not precisely matched to the target tissue or cell type. This can lead to excessive or insufficient expression of the gene product, resulting in unpredictable and potentially harmful effects.

Inappropriate activity levels can cause toxicity or inadequate therapeutic response.

Additionally, the regulation of gene expression is a complex process that involves interactions with various cellular factors. The introduced genes may not interact properly with the cellular machinery responsible for regulating gene expression, leading to uncontrolled or aberrant activity.

This can disrupt normal cellular functions and potentially trigger adverse reactions.

Addressing these technical challenges requires careful design of gene therapy vectors, selection of appropriate regulatory elements, and extensive preclinical testing to ensure controlled and effective gene expression.

The correct option is b. Transferred genes may not have appropriately controlled activity.

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after 24 hours of incubation, you observe 200 colonies present on an lb plate onto which you had previously spread 50 μl of a cell suspension. what is the concentration of the cell suspension?

Answers

Every microbiology experiment requires incubating the plates to encourage microbial growth.

Bacteria that could be harmful to people are less likely to grow while incubating in aerobic conditions and at temperatures below those of the human body. The likelihood that students will open plates when viewing is decreased by taping the lids on, but if there is still a high risk, there are instructions on how to entirely destroy the plates below.

Before incubation, all infected plates must be taped to prevent accidental opening. Fix this by placing two to four small strands of sticky tape at the dish's opposing sides. Complete sealing could encourage the growth of anaerobic germs or hinder normal growth by limiting oxygen diffusion.

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Complete the sentence with the answer choice that is most correct: The climate in the tropical rainforest can be described as...

A.
warm and wet

B.
cold and wet

C.
warm and dry

D.
cold and dry

Answers

The answer is A. Warm and wet :)
The answer would be A warm and wet

What fraction of the moon can be seen from Earth?
A) 1/2
B) 1/4
C) 3/4
D) Whole

Answers

The answer should be 1/2

Help stuck on this one question

Help stuck on this one question

Answers

to be honest I think it's the answer A

How long does it take a water molecule to complete the circuit of
the global conveyor belt?

Answers

Answer:

1,000 years

Explanation:

Select the type of Cell Transport shown in the picture.
Choose

Select the type of Cell Transport shown in the picture.Choose

Answers

Answer:

Osmosis

Explanation:

Osmosis: The movement of water from an area of high concentration to a low concentration through a partially permeable membrane.

Fill In The Blank, relationship status and whether sexual acts have occurred in the past are _________.

Answers

Relationship status and whether sexual acts have occurred in the past are important considerations as these factors can affect the dynamics of a relationship.

Relationship status and whether sexual acts have occurred in the past are important considerations. Both of these factors can affect the dynamics of a relationship, as well as the expectations and boundaries between partners. Some individuals prefer to be in committed, monogamous relationships, while others may prefer casual dating or open relationships.

These preferences can be affected by one's past experiences with sexual activity and relationships, as well as personal values and beliefs. For instance, someone who has been hurt by infidelity in the past may be more cautious about entering into a new relationship, while someone who has had positive experiences with open relationships may prefer that type of dynamic. Ultimately, it is up to each individual to decide what type of relationship they want to pursue, based on their own needs and desires.

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Water intoxication occurs when a. the body's electrolytes are out of balance b. a person has a high body mass in people engaged in low-impact activities d. all of the above​
ITS A I TOOK THE TEST ON EDGE

Answers

Answer:

the answer is: A

Explanation:

Answer:

A).  the body’s electrolytes are out of balance

Explanation: test on edge

hey guys could you help me???

A cell passes on its genetic information ________.
A.
each time it divides
B.
only during certain divisions
C.
before it divides
D.
to neighboring cells

Answers

A. Each time it divides

how does the sexual life cycle increase the genetic variation in a species?group of answer choicesby allowing independent assortment of chromosomesby allowing crossing overall of the given optionsby allowing random fertilization

Answers

The sexual life cycle increases genetic variation in a species through the process of random fertilization. During sexual reproduction, the genetic material from two parents is combined to produce a unique offspring that has a genetic makeup different from either parent.

Independent assortment of chromosomes: During meiosis, the homologous chromosomes pair up and then separate into daughter cells, leading to independent assortment of chromosomes. This means that the chromosome combinations in the gametes produced by each parent are random and different from one another.

Crossing over: During meiosis, homologous chromosomes can exchange segments of DNA through a process called crossing over. This further increases the genetic variation among the offspring by producing new combinations of alleles on the same chromosome.

Random fertilization: When two gametes unite during fertilization, the combination is random, and any one sperm has an equal chance of fertilizing any one egg. This means that the genetic contribution from each parent is random, leading to even more genetic variation in the offspring.

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Which are three requirements for forming petroleum?

Answers

Diagenesis forms Kerogen

Catagenesis (or “cracking”) turns kerogen into petroleum and natural gas

Answer:

i think it is oxygen, heat, steam heat.

Explanation:

which of the following occur as a result of fertilization?
multiple select question.

crossing over haploid organism diploid organism zygote

Answers

The result of fertilization is the formation of a **diploid organism** and a **zygote**.

Fertilization is the process where a sperm cell and an egg cell (both haploid) fuse to form a zygote, which is a single cell with a diploid chromosome number. A diploid organism has two complete sets of chromosomes, one from each parent. This is in contrast to a haploid organism, which has only one set of chromosomes. Crossing over is not a direct result of fertilization; it occurs during meiosis, the cell division process that produces haploid gametes (sperm and egg cells). The zygote formed through fertilization will eventually undergo multiple rounds of cell division and differentiation to develop into a multicellular organism.

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Amplitude of y=sin(x) Find the center of mass of a thin plate of constant density covering the region between the curve y=2sec2x, 4x 4 andthe x-axis. If sin 0 = 4what is cos O?cos 0 ==Simplify your answer if possible. GoPro demonstrated a large number of positive linkages between the 5 Ps and the 5 Cs, especially from the companys perspective. The overall problems demonstrateGroup of answer choicesif a firm can get the 5 Ps right, the corresponding 5 Cs will fall into place.not all of the 5 Ps or the 5 Cs are equally important to the customer.neither the 5 Ps nor the 5 Cs are sufficient to address the marketing challenges of high-tech companies.customers want it right from their perspectives every time. Which Greek mythology character below is 'immortal'?MedusaApolloThe Trojan Horse Grace's retirement party costs $2plus an additional $1 for each guest she invites. What is the maximum numbe of guests there can be Grace can afford to spend a total of $15 on her retirement party? the starting point for preparing the operating activities section using the method is net income. (enter one word per blank.) need help? review these concept resources. Simplify the expression 4m+8+3m as part of the class, we conduct an experiment to see if having me (dr. white) doing the lectures live in class or only watching them on video impacts your grade. in addition, we test whether taking breaks every 15 minutes, every 30 minutes, or no breaks during the 90-minute class plays a role on your grade. the results come in for the 2 x 3 factorial design study and we find that those who are given breaks every 15 minutes do better than those with breaks every 30 minutes or no breaks. this is an example of Jumbo Precast is considering purchasing a machine that will automate the production of its precast concrete products. The cost of the machine is R750 000. This machine could save the company R150 000 per year over the project life of 6 years. The purchase of the new machine will be financed by R300 000 from own company funds and the balance by obtaining a loan of R450 000. The interest payable on the loan is 16% and the loan is paid back over a period of 5 years. According to the SARS, the tax life for this category of equipment is 5 years and the residual value (t=5years) is R750 000. The machine will sell for R150 000 at the end of the 5th year. The company pays 38% tax. Apply straight-line depreciation. The before-tax MARR is 10%. Make a recommendation as to whether the company should purchase the machine. Provide justification for your answer. Which can be found on the periodic table: elements, compounds or mixtures? (a) Name an organism in your area and identity what type of environment it is commonly found in:(b) List some adaptations its has that enable it to exploit certain resources or parts of the environment in whichit lives (e.g. nocturnal, camouflage):(c) What do you know of your organism's niche, i.e. its functional role in the environment? Describe what you know below: Your Task Use the following facts to construct a coherent paragraph with a topic sentence, appropriate supporting sentences, and transitional expressions. - Nearly all teams experience conflict. They should recognize and expect it. - The most effective teams strive to eliminate destructive conflict and develop constructive conflict. - Destructive conflict arises when team members take criticism personally. - Destructive conflict poisons teamwork - Conflict can become constructive. - Teams that encourage members to express their opinions may seem to be experiencing conflict when the opinions differ. - Better decisions often result when teams listen to and discuss many views. WRITE ANESSAYTODESCRIBEHOWDESIGNINNATURECANBEPROTECTEDANDPRESERVEDTOSUSTAINTHELEARNINGOFDESIGNOFDEFFRENTLEVELSOFEDUCATION The collective beliefs, evaluations, and impressions other people hold about an individual are referred to as his or her ______. one of atal hyks most remarkable paintings is a landscape. which statement best describes this ""landscape?"" What is transcriptomics and how does it differ from genomics andproteomics?How is mRNA sequencing different from sequencing genomicDNA? A human who has no access to fresh water but is forced to drink seawater instead will ________.passively excrete excess water in order to remove the high concentration of ingested salt Debts owed by a business are referred to as:_______ a. expenses b. stockholders' equity c. accounts receivables and.liabilities Suppose X is a normal random variable with = 70 and = 5. Find the values of the following probabilities. (Round your answers to four decimal places.)P(66 < X < 76)