How do you prepare 300 ml buffer of 100 mm tris ph 7. 8 and 250 mm nacl?
Answers
A buffer of 300 ml of 100 mM Tris pH 7.8 and 250 mM NaCl can be prepared by dissolving 3.64 g of Tris and 4.27 g of NaCl in 300 ml of water, and adjusting the pH to 7.8 using 10 ml of 1 M HCl. The % v/v refers to the volume of the solute while % w/v refers to the weight of the solute.
To prepare a 300 ml buffer of 100 mM Tris pH 7.8 and 250 mM NaCl, you need to follow the following steps:1. Calculate the amount of Tris required to prepare 100 mM solution of Tris, which is equal to 100 mM x 0.3 L = 0.03 moles. The molecular weight of Tris is 121.14 g/mol. Thus, the amount of Tris required is 3.64 g.2. To make the buffer of pH 7.8, use HCl or NaOH to adjust the pH. For this, use 1 M HCl or 1 M NaOH to avoid diluting the buffer. Add 10 ml of 1 M HCl to the solution.3. Measure 4.27 g of NaCl and add it to the solution. 4. Add water to the solution to make up the final volume of 300 ml. 5. Mix the solution thoroughly until everything is dissolved. Your buffer of 100 mM Tris pH 7.8 and 250 mM NaCl is now ready. % v/v refers to the percentage volume of a solute in a solvent while % w/v refers to the percentage weight of a solute in a solvent. The percent v/v is calculated by the volume of the solute divided by the volume of the solution while the percent w/v is calculated by the mass of the solute divided by the volume of the solution in which it is dissolved.For more questions on buffer
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The correct question would be as
How do you prepare 300 ml buffer of 100 mM Tris pH 7.8 and 250 mM NaCl? % v/v,% w/v Questions.
Related Questions
why is my family dissapointed in me?
Answers
Answer:
literally same im failing almost every class and i just know my dad hates me because of it
Explanation:
- Lead acid batteries used in cars contain a concentrated
solution of sulfuric acid. When these batteries are
recycled, their sulfuric acid is drained and neutralized to
produce water and sodium sulfate. Suggest a compound
that could be used to neutralize sulfuric acid and produce
sodium sulfate. Justify your choice by writing the chemical
equation for the reaction.
Answers
As per the chemical reaction ,sodium hydroxide can be used for neutralization of sulfuric acid as,2 NaOH+H₂SO₄\(\rightarrow\)Na₂SO₄ +2 H₂O.
What are chemical reactions?Chemical reactions are defined as reactions which occur when a substance combines with another substance to form a new substance.Alternatively, when a substance breaks down or decomposes to give new substances it is also considered to be a chemical reaction.
There are several characteristics of chemical reactions like change in color, change in state , change in odor and change in composition . During chemical reaction there is also formation of precipitate an insoluble mass of substance or even evolution of gases.
There are three types of chemical reactions:
1) inorganic reactions
2)organic reactions
3) biochemical reactions
During chemical reactions atoms are rearranged and changes are accompanied by an energy change as new substances are formed.
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Animal cells do NOT
have cell walls. Why do
you think this is an
advantage?
Answers
Answer:
Because they don't need them , cell wall which are found in cell, maintain cell shape almost as if each cell has it's own exoskeleton ame this them to stand upright without the need for bones .
Explanation:
The atoms of elements A (blue) and B (orange) form 2 compounds shown here. Do these compounds obey the law of multiple proportions?
Answers
Hello. You have not presented the image that presents the two elements mentioned in the question. This makes it impossible for your question to be answered. However, I will try to help you as best I can.
To say whether the compounds obey the law of multiple proportions, you should observe whether one of the elements, formed from the compounds, maintains a fixed mass, while the other element presents the mass in a varied amount of small, whole numbers, spread across the formed compounds .
This is because the law of multiple proportions states that an element must have a fixed mass when reacting with another element to create compounds. This reaction will allow this element to keep the mass fixed, while the other element will generate different compounds, where each one presents a part of the mass of the forming element, in small and whole numbers.
of the two lewis/electron dot structure representations, which is the correct one for phosphorus trifluoride?
Answers
The correct lewis/electron dot structure is given below
What is lewis electron dot structure ?The diagrams known as Lewis structures, often referred to as Lewis dot formulas, Lewis dot structures, electron dot structures, or Lewis electron dot structures, depict the interactions between the atoms in a molecule as well as any lone pairs of electrons that may be present.
The valence shell electrons of a molecule are shown in an extremely simplified manner by a Lewis Structure. It is used to demonstrate how the electrons in a molecule are positioned around particular atoms. Electrons are shown as "dots" or, in the case of a bond, as a line connecting the two atoms.
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. The model shows Earth in several positions relative to the Sun. In which position is it winter in North America?
Answers
When a substance dissolved in a liquid, its dippears? truth or false
Answers
Answer:
False, I doesn't just disappear into thin air it dissolved so it mixes with the other thing. So now it is transparent AKA see through
Explanation:
Help meeeeee
A 280.0 sample of neon gas exerts a pressure of 660.0 mm Hg at 26.0°C. Assuming that the pressure remains constant, at what temperature in would the volume change to 440.0 ml?
Answers
Answer:
469.9K
Explanation:
Using Charles's law equation:
V1/T1 = V2/T2
Where;
V1 = initial volume (ml)
V2 = final volume (ml)
T1 = initial temperature (K)
T2 = final temperature (K)
Based on the information provided:
V1 = 280.0ml
V2 = 440.0 ml
T1 = 26.0°C = 26 + 273 = 299K
T2 = ?
Using V1/T1 = V2/T2
280/299 = 440/T2
0.936 = 440/T2
T2 = 440 ÷ 0.936
T2 = 469.9K
Which one of the following is least likely to be an elementary step in a reaction mechanism?NO2(g) + O3(g) → NO3(g) + O2(g)C2H6(g) + 5O2(g) → 2 CO2(g) + 3 H2O(g)2NO(g) + O2(g) → 2 NO2(g)2HI(g) → H2(g) + I2(g)NO3(g) + NO(g) → 2 NO2(g)
Answers
The second reaction, C2H6(g) + 5O2(g) → 2 CO2(g) + 3 H2O(g), is least likely to be an elementary step in a reaction mechanism. This is because it involves the simultaneous collision of seven molecules, which is very unlikely to occur in a single step.
Elementary steps usually involve the collision of one or two molecules, or the breaking or formation of a bond between two atoms or molecules. The other reactions listed all involve one or two molecules colliding, or the breaking or formation of a bond between two atoms or molecules, and are more likely to be elementary steps in a reaction mechanism.
This is because the probability of multiple molecules colliding simultaneously is lower than that of a single molecule colliding with another molecule.
Therefore, the second reaction listed, C2H6(g) + 5O2(g) → 2 CO2(g) + 3 H2O(g), is least likely to be an elementary step in a reaction mechanism due to the large number of particles involved in the collision. Instead, this reaction is likely to proceed through a series of multiple elementary steps that involve the sequential breaking and formation of multiple bonds between atoms and molecules.
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What causes hurricane on Saturn?
Answers
Hurricanes on Saturn are caused by when the water clouds heat up and rise, causing storms (not too sure if this is the right answer, hope it helped).
calculate the mass of 0.5 moles of caco3 in grams with procedure
Answers
Answer:
50g
Explanation:
moalr mass of CaCO3= 40g/mol+ 12g/mol+(16g/mol*3)
=52g/mol+48g/mol
=100g/mol
1 mol= 100g
0.5mol=x
by cross multiplication,
0.5*100gmol=x mol
x=50g
ethane is a substance that is used when making plastic. a molecule of ethane is made of two carbon atoms and six hydrogen atoms. the carbon atoms are joined by the chemical bond. each of the hydrogen atoms is joined to one of the carbon atoms by a chemical bond. which is an accurate model of ethane?
Help ASAP!!!
Answers
Answer:
C
Explanation:
Option C is an accurate model of ethane.
Ethane is an alkane that has two carbon atoms bonded together and each atom has three hydrogen atoms that are attached to them. Ethane has single bonds and that makes it an alkane.
This model of ethane has six carbon-hydrogen bonds shown with solid lines that are protruding out. Rotation that takes place about the carbon-carbon bond actually results in the different molecular conformations of ethane.
What is the electron configuration for a neutral atom in the ground state with a total of 6 valence electrons?
Answers
The electron configuration for a neutral atom in the ground state with a total of 6 valence electrons is 1s²2s²2p⁴, option A.
The distribution of electrons in an element's atomic orbitals is described by the element's electron configuration.
Atomic electron configurations adhere to a standard nomenclature in which all atomic subshells that contain electrons are arranged in a sequence with the number of electrons they each possess expressed in superscript. For instance, sodium's electron configuration is 1s22s22p63s1.
For a neutral atom such as oxygen , whose atomic number is 8 , has 8 electrons .2 electrons are present in its 1st shell and 6 valence electrons are present in its outermost shell or 2nd shell , such that the electronic configuration of the atom becomes 1s²2s²2p⁴.
The arrangement of an atom's or molecule's electrons in atomic or molecular orbitals is known as the electron configuration in atomic physics and quantum chemistry.
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Complete question:
Which is the electron configuration of a neutral atom in the ground state with a total of six valence electrons?
1) 1s^2 2s^2 2p^4
2) 1s^2 2s^2 2p^6
3) 1s^2 2s^2 2p^2
4) 1s^2 2s^2 2p^6 3s^2 3p^6
50 POINTS!! CAN SOMEONE PLEASEEEE HELP ME do this for my chemistry class please i need it done asap!! to give me the answers your probably going to have to write it on paper then attach the picture to your answer!! thank you so much!! <3
Answers
The chlorine atom only requires one more electron to fulfill its octet because the potassium atom only has one valence electron and has seven total electrons.
How do atoms work?Protons, positive charged particles, and neutrons, neutral charged particles, make up the nucleus of each atom. Around the nucleus are electrons, which are negative particles. Atoms of the same element share the same chemical characteristics and mass, but atoms of other elements have unique chemical characteristics and masses. Compounds are created when atoms join in ratios of few whole numbers.
Describe a compound?A compound is a substance made up of two or more types of elements together in a specific proportion of their atoms. As the components are combined, they interact with one another.
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0. 75 moles of hf and 1. 00 mole of naf were added to 1. 00 l of water. To this solution was added 0. 200 mol of naoh. What was the final ph of the solution?.
Answers
what is the mass of the block of iron illistrated below
Answers
The mass of the iron object is 787.4 g.
What is density?The term density has to do with the mass per unit volume of the object. This implies that when we divide the mass of the object by the volume of the object, we get the density of the object.
We also have to note that the density is an intrinsic property of an object could be used as a means that can be harnessed in the process of trying to identify the unknown material that we have.
In this case we have the thorough depiction of the iron as we can see from the image attached and we want to find the mass of the object.
Volume of iron = 2 cm * 10 cm * 5 cm = 100 cm³
Density of iron = 7.874 g/cm³
Mass of iron = Volume * density = 100 cm³ * 7.874 g/cm³ = 787.4 g
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the diels-alder mechanism between a diene and a dienophile is choose... , which means that bond breaking happens choose... bond forming. to help the mechanism succeed, the diene should have an choose... group and the dienophile should have an choose... group.
Answers
The Diels-Alder mechanism among a diene and a dienophile is a concerted reaction, because of this that bond breaking occurs simultaneously with bond forming.
To help the mechanism be triumphant, the diene have to have an electron liberating organization and the dienophile have to have an electron taking flight organization.
A concerted reaction is a form of chemical response where all of the bond-forming and bond-breaking steps arise concurrently, in a single step. In other phrases, all of the reactants come collectively and react to shape the products with none intermediate steps.
Concerted reactions also are referred to as pericyclic reactions due to the fact they contain the formation and breaking of bonds in cyclic transition states. these reactions comply with a specific set of rules referred to as the Woodward-Hoffmann guidelines, which describe the allowed and forbidden modes of overlap among the orbitals of the reactants and products. These reactions are often utilized in organic synthesis to create complicated molecules with excessive stereoselectivity and regioselectivity.
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Complete Question:
The Diels-Alder mechanism between a diene and a dienophile is _____, which means that bond breaking happens _____ as bond forming.
To help the mechanism succeed, the diene should have an _____ group and the dienophile should have an ______ group.
Given the equilibrium system at 25°C:NH4Cl(s) = NH(aq) + Cl(aq)(AH = +3.5 kcal/mole)Which change will shift the equilibrium to the right?
Answers
Explanation
NH4Cl(s) = NH4+(aq) + Cl-(aq) AH = +3.5 kcal/mole
We have some changes that shift the equilibrium to the right, according to Le Châtelier's principle.
I will write some of them:
By Adding amount of NH4Cl or by increasing the temperature because according to the heat of the reaction this is an endothermic reaction.
Answer: Increasing the temperature of the system.
what is the effect on the concentrations of no-2, hno2, oh and when is added to a solution of in water. the equation for the equilibrium is:
Answers
When water is added to a solution with NO2- and HNO2, the concentration of NO2- will increase, the concentration of HNO2 will decrease, and the concentration of OH- will increase.
The addition of water to a solution will have different effects on the concentrations of NO2-, HNO2, and OH-.
1. NO2- (nitrite ion):
When water is added to the solution, it will dissociate into H2O and NO2-. This will increase the concentration of NO2- in the solution, as water acts as a source of NO2- ions.
2. HNO2 (nitrous acid):
The equilibrium equation suggests that HNO2 is formed when NO2- reacts with H2O. However, the addition of water will cause the concentration of HNO2 to decrease. This is because the reaction between NO2- and H2O favors the formation of NO2- rather than HNO2. So, the increase in the concentration of NO2- will decrease the concentration of HNO2.
3. OH- (hydroxide ion):
The addition of water will increase the concentration of OH-. Water naturally contains OH- ions, so the presence of water will increase the concentration of OH- in the solution.
changes occur due to the equilibrium between NO2- and HNO2, as well as the dissociation of water to form OH- ions.
It's important to note that the specific changes in concentrations will depend on the initial concentrations of NO2-, HNO2, and OH- ions, as well as the equilibrium constants involved in the reactions.
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What tool first allowed scientists to study cells?
Answers
Answer: Microscope
Robert Hooke's Microscope. Robert Hook refined the design of the compound microscope around 1665 and published a book titled Micrographia which illustrated his findings using the instrument.
Explanation:
Answer:
Hey i actually know this! haha only cause i did it.
its a Microscope.
and im pretty sure hooke was the first to discover!
Explanation:
brainliest?
A science student wants to use distillation to separate a mixture of two substances in the liquid state. For the student to be successful, which property should be significantly different between the two liquids?
Answers
For a science student to be successful in separating a mixture of two substances in the liquid state using distillation, the boiling points of the two liquids should be significantly different.
What happens during a distillation?In distillation, a mixture of liquids is heated, and the vapor produced is condensed and collected. The liquids in the mixture will have different boiling points, so the substance with the lower boiling point will vaporize and condense at a lower temperature compared to the substance with the higher boiling point.
What is necessary for a successful distillation?For a successful distillation, it is important that the boiling points of the two liquids are significantly different so that they can be separated easily. If the boiling points of the two liquids are too close, it will be difficult to separate them using distillation.
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What name is given to a material that does not conduct thermal energy well?
Answers
The name given to a material that does not conduct thermal energy well is an insulator.
Thermal energy is the internal energy contained in a substance, which increases with the average velocity of the constituent particles, which may be atoms or molecules. The faster the molecules move, the greater the thermal energy. Thermal energy may be transferred between objects, but it is only transferred from a hotter to a cooler object. For example, heat is transferred from hot coffee to the mug holding it, then from the mug to the air in the room if the mug is left on a table. An insulator is a material that is used to insulate, such as electrical wires or pipelines. An insulator is a material that does not conduct thermal energy, electricity, or sound well. For example, rubber is a good insulator because it does not conduct electricity, while air is a good insulator because it does not conduct heat. Apart from rubber, other good thermal insulators are foam, wool, glass wool, and mineral wool. Thermal insulation is used in walls, windows, doors, roofs, and floors to keep buildings warm in winter and cool in summer.Learn more about Thermal energy: https://brainly.com/question/19666326
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In the painting : Let My People Go by Aaron Douglas
What type of palette did the artist use in the painting? (analogous, limited, open, monochromatic)
What types of colors are used in the work of art? (full-intensity, low-key (shades), or high-key (tints))
Where is the light source in the work of art? Where are the shadows located in your work of art?
Did the artist use asymmetrical, symmetrical, or radial balance?
What is emphasized in the work of art?
What is the size of the objects or figures in the work of art? (normal (life-size), small, colossal)
Answers
Aaron Douglas was a prominent figure of the Harlem Renaissance, a cultural movement that emerged in the 1920s and 1930s.
Who is Aaron Douglas?Based on his style, it is likely that Douglas used a limited palette in his painting Let My People Go. He often used a muted color scheme, with earthy tones and blues, greens, and purples.
Douglas typically used high-key colors in his paintings, which means that he used bright, saturated colors that create a sense of energy and vitality. He also used low-key colors to create contrast and depth.
The light source in Douglas' paintings is often not clearly defined, as he tended to flatten the picture plane and eliminate shadows. When shadows are present, they are often stylized and simplified.
In terms of balance, Douglas often used symmetrical and asymmetrical compositions in his paintings. He was also known for using repetition and pattern to create a sense of visual harmony.
In Let My People Go, it is likely that Douglas emphasized the figures and their struggle for freedom. The painting depicts the biblical story of Moses leading the Israelites out of slavery in Egypt, which was a powerful symbol of hope and liberation for African Americans during the Harlem Renaissance.
Finally, the size of objects and figures in Douglas' paintings varied depending on the composition and the message he wanted to convey. He often used large, monumental figures to emphasize their strength and resilience.
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A(n) ______ is the interaction that allows for atoms to join together.
chemical bond
molecular formula
electron cloud
valence electron
Answers
Answer:
chemical bond
Explanation:
chemical bond is the interaction that allows for atoms to join together
Answer:
c. A electron cloud is the interaction
With regard to tonicity, two solutions that have the same concentrations of nondiffusible solutes and water are said to be?
Answers
Any external solution that contains the same amounts of solutes or water as biological fluids is seen to be isotonic. The flow of water inside an isotonic solution won't be net.
Which fatty acids are present in nature Mcq most frequently?The 16- or 18-carbon fatty acids, also known as palmitic acid or stearic acid, respectively, are among the most widely dispersed fatty acids. The bulk of organisms' lipids contain both and stearic acids. In animals, up to 30% of body fat is made up of palmitic acid.
What is the fatty acid's Mcq solubility in water?Water only partially dissolves fatty acids. The hydrophilic COOH group just at end of the hydrocarbon chain is what causes the partial solubility. The hydrocarbon chains cannot be dissolved at all in water.
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Which pair of diseases could both be treated effectively with antibiotics
A. athletes foot and flu
B. cholera and salmonella
C. flu and scurvy
D. malaria and rickets
Imma mark the correct answer brainliest
Answers
Answer:
cholera and salmonella
Explanation:
i believe so
What is solubility a measure of?
A. The amount of solute that will dissolve
B. The type of solute that will dissolve
C. The rate at which the solute will dissolve
D. The energy needed for the solute to dissolve
Answers
Answer:A
Explanation:
I just took the test
What is the only thing that changes in a chemical reaction
Answers
How many moles are in 14.0156 grams of C3H12?
Answers
Answer:
Explanation:
Molar Mass of C3H12 (I don't think this chemical exists anywhere but on paper so I'll solve it that way, but check to see if you have written the question correctly.)
3C = 3 * 12 = 36
12*H = 12*1 = 12
Total = 48 grams
mols = given mass / molar mass
Given mass = 14.0156 grams
Molar mass = 48 grams
moles = 14.0156 / 48
mols = 2.900 mols
Notes
When you enter your answer, be sure and include the zero for sig digs.You must clean up my numbers (make them more accurate) using your periodic table.what occurs when aqueous silver nitrate, a g n o 3 , reacts with aqueous potassium sulfate, k 2 s o 4 ?
Answers
Answer: A white precipitate of silver sulfate (Ag2SO4) is formed.
Explanation:
When aqueous silver nitrate (AgNO3) reacts with aqueous potassium sulfate (K2SO4), a double displacement reaction occurs. The cations and anions of the two compounds switch places to form two new compounds, which are potassium nitrate (KNO3) and silver sulfate (Ag2SO4).
AgNO3 + K2SO4 → Ag2SO4 + 2KNO3
The insoluble product of this reaction is silver sulfate (Ag2SO4), which appears as a white precipitate. This reaction is commonly used to detect the presence of sulfate ions in solution, as the formation of the silver sulfate precipitate confirms the presence of sulfate ions.