establishment of a resazurin-based aortic valve tissue viability assay for dynamic culture in a microphysiological system

Answers

Answer 1

The statement refers to the establishment of a resazurin-based aortic valve tissue viability assay for dynamic culture in a microphysiological system.

The aortic valve is a cardiac valve that regulates blood flow from the left ventricle to the aorta. Aortic valve tissue refers to the specialized tissue that makes up the valve. A tissue viability assay is a method used to assess the health and viability of living tissues.  Resazurin is a dye that can be reduced by metabolically active cells, producing a fluorescent signal proportional to cell viability.A microphysiological system, often referred to as a "organ-on-a-chip" system, is a miniaturized platform designed to mimic the structure and function of human organs or tissues.

The aortic valve tissue is cultured in a dynamic environment that mimics the physiological conditions experienced by the tissue in the body.

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Related Questions

a scientist is studying the role of variable temperature on the species composition of an alpine meadow. this is a study at what level of ecology?

Answers

The scientist studying the role of variable temperature on the species composition of an alpine meadow is conducting a study at the community level of ecology.

This level of ecology is concerned with understanding the interactions between different species within a defined geographic area. The community level includes studies of biodiversity, species interactions, and the role of abiotic factors, such as temperature, in shaping the composition and distribution of species within a community. In this case, the scientist is investigating how changes in temperature may affect the species composition of the alpine meadow community.

This is a complex question that requires a because it involves multiple ecological concepts and requires an understanding of the different levels of ecological organization.

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Which of the following parts of a Chick-Fil-A meal is an example of a lipid?


The chicken in the chicken sandwich

The peanut oil used to fry the waffle fries.

The potato in the waffle fries

The bread in the sandwich

Answers

Hello,

A LIPIDS is a fatty acid, this also includes natural oils and waxes. Usually meat has lipids.

Choice B is incorrect because fries don’t have meat which don’t have any lipids

Choice C is incorrect because potato have no meat so there is not any lipids

Choice D is incorrect because bread has no meat so there is not any lipids

Choice A is correct because meat has lipids

CORRECT ANSWER:


A) The chicken in the chicken sandwich

Hope this helps!!
Brainliest??

The picture below demonstrates which type of chromosome mutation?

The picture below demonstrates which type of chromosome mutation?

Answers

Answer:

I feel like it's a Translocation Mutation

Explanation:

( :

Why is decreased genetic variation in a species considered a negative impact of genetic technologies?
o It reduces the nutritional value of foods produced by these species,
It produces crops that are more resistant to weed killer.
It makes the species more susceptible to diseases,
It results in foods with less desirable traits,

Answers

Answer:

it make species more susceptible to disease

Explanation:

There are two forms of reproduction: asexual and sexual.

- asexual organisms: if a disease were to spread in organisms that spread asexually would most likely spread to all of them because of the lack of genetic variation.

>if there were a hundred phones with the same password and you found out that password you can hack into 100 phones.

- sexual organisms: the disease is less likely to spread.

>if there were a hundred phones with all different passwords it would one take way longer to hack into them into overtime they could catch on and change their password (genetic variation)

Decreased genetic variation in species is considered a negative impact of genetic technologies because it makes the species more susceptible to diseases.

Genetic variation is a prerequisite for both natural selection and artificial selection.

In artificial selection, the genetic crosses aimed at obtaining individuals showing desired phenotypic traits are known to decrease the genetic variation.

This decrease in genetic variation may be associated with different problems in offspring across generations (e.g., susceptibility to diseases).

In conclusion, decreased genetic variation in species is considered a negative impact of genetic technologies because it makes the species more susceptible to diseases.

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complete the following statements to describe several genetic disorders and their symptoms. choices can be used more than once.

Answers

Genetic disorders are a diverse group of conditions caused by abnormalities in an individual's genetic material. Some examples of genetic disorders and their associated symptoms include Down syndrome.

Down syndrome is a genetic disorder caused by the presence of an extra copy of chromosome 21. Individuals with Down syndrome often exhibit intellectual disabilities, developmental delays, and characteristic physical features such as slanted eyes, a flat facial profile, and a short neck. They may also experience heart defects, hearing and vision problems, and a higher risk of certain health conditions.

Cystic fibrosis (CF) is an inherited disorder caused by mutations in the CFTR gene. CF affects multiple organ systems, primarily the lungs and digestive system. Symptoms can include persistent coughing, frequent lung infections, poor growth and weight gain, digestive problems such as malabsorption and pancreatic insufficiency, and salty-tasting skin. CF can also lead to complications such as diabetes, liver disease, and fertility issues.

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A small polar molecule enters a cell with help from a membrane protein. No
additional energy was needed to complete this process. What type of
transport is this?
OA. Facilitated
OB. Active
OC. Osmosis
D. Exocytosis

Answers

It is an facilitated kind of transportation.

The cell membrane is accessed by tiny, polar molecules in what way?

Although many polar molecules, including glucose, can pass through cell membranes, ions and the majority of polar molecules cannot. Certain transmembrane proteins function as transporters to allow these compounds to traverse membranes.

What class of membrane proteins permits polar molecules to enter?

Response and justification A transport protein is a type of membrane protein that is involved in moving ions and polar molecules across the membrane. Protein pumps and channel proteins are two different classes of transport proteins that carry out this function.

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Which of the following describes the
coastal photic zones?
A. permanent darkness below 1000 meters with
bioluminescent bacteria, bottom feeders, and angler fish
B. up to 200 meter depth and includes photosynthetic
plants, sea anemones, sponges, crabs, and clams
C. the "twilight zone" between 200-1000 meters deep
and includes whales and octopi and little life

Answers

Photic zone up to 200 meter depth and includes photosynthetic plants, sea anemones, sponges, crabs, and clams. The correct option is B.

What is photic zone?

The photic zone, also known as the euphotic zone, epipelagic zone, or sunlight zone, is the uppermost layer of a body of water where phytoplankton can perform photosynthesis.

It goes through a number of physical, chemical, and biological processes that allow nutrients to enter the upper water column.

The photic zone is the top layer of the ocean, closest to the surface, and is also known as the sunlight layer. Enough light penetrates the water in this zone to allow photosynthesis.

Thus, the correct option is B.

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Veins contain a network of valves to ensure a one-way flow of blood, but arteries do not. This is because veins must counteract the force of.

Answers

Veins contain a network of valves to ensure a one-way flow of blood, but arteries do not. This is because veins must counteract the force of gravity.

Veins, unlike arteries, have valves that ensure blood flows in only one direction. Arteries do not need valves because the pressure from the heart is so high that blood can only flow in one direction. Valves also assist blood in traveling back to the heart against gravity.

Veins are an important part of the circulation system, which transports blood throughout the body. Most veins transport deoxygenated blood from bodily tissue to the heart, where it is oxygenated. To return blood to the heart, veins must work against gravity. The blood that flows through the veins is deoxygenated and at low pressure. It moves slowly and steadily in the opposite direction of gravity. As a result, veins have valves to prevent blood backflow.

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How are soluble food molecules absorbed into the epithelial cells of the small intestine

Answers

Soluble food molecules are absorbed into the epithelial cells of the small intestine through the process of diffusion and active transport.

Where does all the energy of a food chain start?

Answers

from the sun
then the producers which are the plants
hope this helps :)

Define kinesiology and list seven types of science it encompasses

Answers

Answer:

Kinesiology is the study of human or non-human body movement. The seven types of sciences it encompasses are physiology, anatomy, biomechanics, psychology, sociology, motor learning and sports pedagogy.

Why testes is extra abdominal in position in man ?​

Answers

Extra-abdominal position means that the testes hang outside the abdomen and are not in it. This is important because the sperms in the testes are very susceptible to high temperatures - meaning that they can easily die due to high temperatures like what we have within the abdomen

According to the graph, which of the following populations has likely reached it's carrying capacity?

(Option A): Population A
(Option B): Population B
(Option C): Population C
(Option D): Population D

According to the graph, which of the following populations has likely reached it's carrying capacity?(Option

Answers

Answer:

population A

Explanation:

because, it starts at 1 so it starts when the first person started the population, and it has a constant rate of change

what is the wavelength of a wave with the speed of 680 m/s and the frequency of 400 hertz

Answers

Answer:

Wavelength of the wave is \(1.7\) meter

Explanation:

As we know that

\(c = \lambda *v\)

C is the speed of light

\(\lambda\) is the wavelength of light

v is the frequency of the light

Substituting the given values in above equation, we get -

\(680 = \lambda * 400\)

\(\lambda = \frac{680}{400}\\ \lambda = 1.7\) m

Which system(s) are represented in this image?

A. Respiratory
B. Skeletal
C. Endocrine
D. Muscular

Which system(s) are represented in this image?A. Respiratory B. SkeletalC. EndocrineD. Muscular

Answers

Answer:

I would say it looks like muscular, but Im not totally sure

Hope its the right answer though, :)

Explanation:

please help, it's my last question ​

please help, it's my last question

Answers

Answer:

As it gets closer to the sun.

Explanation:

Hope this helps and one of the mods doesn't take it down.

Answer:As it gets closer to the sun

Explanation:

A very small amount of Earth's vast water supply is available to humans and other species as liquid fresh water.

Answers

Around 15% is available to us

What is the first component of the naturally occurring CRISPR system?

Answers

Answer:

Crispr cas9 Basics

Explanation:

Which factors best determine the variety of climate zones on earth?

Which factors best determine the variety of climate zones on earth?

Answers

I’m pretty sure the answer is B

An age pyramid with a wide base shows a population that
A. Declining
B. Growing
C. Stabilized

Answers

C is shows all ages in that country or area and which is declining as which is growing

5. Why does DNA replicate during the S phase?

Answers

Because if not u would not be who u are

Answer:

The S phase of a cell cycle occurs during interphase, before mitosis or meiosis, and is responsible for the synthesis or replication of DNA. In this way, the genetic material of a cell is doubled before it enters mitosis or meiosis, allowing there to be enough DNA to be split into daughter cells.

Explanation:

what are the levels of organization in a living thing

Answers

Answer:cells

Explanation:cells

Ms.Caroll went to the doctors office with pain in her right shoulder blade and learned she was having a gallbladder attack. Youre not surprised to hear about that unusual location of her pain since its known as _____.
A- Chronic pain
B- Referred pain
C- Receptive field
D- Phantom limb pain

Answers

Answer:

B. Referred pain

Explanation:

Referred pain is when pain in one part of your body is caused by pain in another part of your body.

The pain that Ms. Caroll is experiencing in her gallbladder is causing her to experience pain in her right shoulder blade.

Also, when the gallbladder is inflamed and swollen, it irritates the phrenic nerve which runs from the abdomen, through the chest, and into the neck. This causes pain in the right shoulder blade.

Hope this helps.

A man is better than a woman in 450 words essay writing

Answers

Goodday mr.chairman panels of judges accurate time keeper and my fellow listeners.

my name is kuku Zainab am here to oppose the motion which says a man is better than a woman.

A woman is an adult of female,a woman is the one that take care of a family. a woman is a person that did house chores these are washing the dishes, washing the cloth, mopping, washing the toilet, cooking and sweeping the floor which are not easy to do those House work.why A man is the one that provides for the family , only they no to do is to be pressing phone when woman are doing there house chores.

1. A group of students (group 1) perform an experiment to determine the concentration of coliforms in potable water. In this experiment,5 mLof water was filtered and the membrane was incubated on mFC agar plates at37∘C. After 24 hours, the plate was observed for colonies. mFC agar is a selective media that allows for the growth of coliforms. On mFC agar, fecal coliforms form blue colonies and non-fecal coliforms form white colonies. The mFC agar contains selective and differential agents that allow for growth and identification of coliforms. Rosolic acid inhibits bacterial growth in general, except for growth of coliforms. Bile salts inhibit contaminating gram positive bacteria. Aniline blue indicates the ability of fecal coliforms to ferment lactose to acid that causes a pH change in the medium. Lactose utilization (blue color) is the basis for identification of fecal coliforms. A. (1 pt) From the picture above, calculate the number (in CFU) of total coliforms and the number of fecal coliforms in the5 mL sample of water. Be sure to include units in your answer Non-fecal coliform are
1CFU/mL
and fecal coliform are
4CFU
B. (1 pt) Now, calculate the concentration (in CFU/100 mL) of total coliforms in the water sample. Our standard for reporting is CFU/100mL. Keep in mind that you have only filtered 5
mL
of water, but you have to calculate the number of organisms in
100 mL
, There are two ways to do this: (1) use the formula in the protocol, or (2) consider that
100 mL
is
10X
greater than
10mh
Be sure to include units in your answer. C. (0.5 pt) Would you drink this water? Why or Why not? D. (0.5 pt) Why do non-fecal coliforms form white colonies while fecal coliform formed blue colonies in the above picture? E.(0.5pt) If you were to gram stain one of the blue colonies on the plate, what would you predict for the results of that gram stain? Include shape, color, and what the gram stain color indicates.

Answers

A. Based on the information provided, the number of total coliforms in the 5 mL sample of water is 5 x 1 CFU/mL = 5 CFU,

the number of fecal coliforms is 1 x 4 CFU = 4 CFU.

B. the concentration of total coliforms in the water sample is 288 CFU/100 mL.

C. Based on the high concentration of coliforms in the water sample, it is not safe to drink.

D. Non-fecal coliforms form white colonies because they do not ferment lactose to produce acid, and therefore do not cause a pH change in the medium that would result in a blue color.

E. If we were to gram stain one of the blue colonies on the plate, we would predict that it is a gram-negative bacterium.

Coliform Concentration Calculation

A. Based on the information provided, the number of total coliforms in the 5 mL sample of water is 5 x 1 CFU/mL = 5 CFU,

the number of fecal coliforms is 1 x 4 CFU = 4 CFU.

B. To calculate the concentration of total coliforms in CFU/100 mL, we can use the following formula:

Concentration (CFU/100 mL) = (Number of colonies / Volume plated) x (Dilution factor / Volume filtered) x 100

In this case, the dilution factor is 20 (assuming a 1:20 dilution was used), and the volume filtered is 5 mL. To calculate the volume plated, we need to know the diameter of the membrane filter used. Let's assume it was 47 mm, which corresponds to a surface area of approximately 17.36 cm^2. Using a conversion factor of 1 cm^2 = 0.1 mL, we can estimate the volume plated as:

Volume plated = Surface area x Depth of agar = 17.36 cm^2 x 0.2 cm = 3.472 mL

Plugging in the values, we get:

Concentration (CFU/100 mL) = (5 / 3.472) x (20 / 5) x 100 = 288 CFU/100 mL

Therefore, the concentration of total coliforms in the water sample is 288 CFU/100 mL.

C. Based on the high concentration of coliforms in the water sample, it is not safe to drink. Coliforms are indicators of fecal contamination and their presence in drinking water can indicate the presence of harmful pathogens.

D. Non-fecal coliforms form white colonies because they do not ferment lactose to produce acid, and therefore do not cause a pH change in the medium that would result in a blue color. Fecal coliforms, on the other hand, can ferment lactose and produce acid, which results in the blue color of their colonies.

E. If we were to gram stain one of the blue colonies on the plate, we would predict that it is a gram-negative bacterium. Gram-negative bacteria typically appear pink or red after staining, and this color indicates that the bacteria have a thin peptidoglycan layer in their cell walls and an outer membrane containing lipopolysaccharides. The shape of the bacteria would depend on the species, but coliforms are generally rod-shaped (bacilli).

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POSSIBLE POINTS 3
A cell whose cytoplasm has a concentration of 2% glucose is placed in a test tube containing a 1% glucose solution. How would you describe the
solution outside the cell?
hypertonic
isotonic
hypotonic
saturated

Answers

Hypotonic - because there is a higher concentration inside the cell than outside of it

What is true for both endocytosis and exocytosis?

A) Both take substances into the cell.

B) Both release signaling molecules.

C) Both decrease the surface area of the cell membrane.

D) Both require energy.

What is true for both endocytosis and exocytosis?A) Both take substances into the cell.B) Both release

Answers

Answer:

Both require energy.

Explanation:

Because they transport substances into and out of the cell by active transport that requires energy

onsuming foods with high glycemic index immediately after a bout of exercise will multiple choice question. ensure a slow rise in blood glucose. supply amino acids for muscle repair. rapidly restore glycogen stores. replenish sweat losses.

Answers

Consuming foods with high glycemic index immediately after a bout of exercise will c. rapidly restore glycogen stores.

Consuming foods with a high glycemic index such as boiled potatoes, white rice, white bread after exercise can quickly restore glycogen stores that are depleted during exercise. The glycemic index value depends on several factors, including dietary fiber content, amylose and amylopectin levels, fat and protein content, starch digestibility, and processing methods.

Glycogen is a storage form of glucose, where glucose is a simple sugar, a form of carbohydrates. Glycogen is an important fuel for athletes, because it is quickly broken down and used for energy.

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The text below was part of an article in a teenage magazine:


‘Young people today are far too materialistic.’

Do you agree?

Answers

Answer:

That's um...well how do I say this, terrible. They're saying it's a horrible thing but I think not, everyone does different things and I respect that. So it's best not to judge someone by what they're doing even in a magazine.

how does an entire population become adapted to its environment

a. traits that help individuals survive and reproduce become more common in the population over successive generations
b. each new variation becomes an adaptation as the organisms learn to use it
c. a population chooses which traits will help it survive
d. individuals develop changes during their lifetime and pass good changes to their offspring

Answers

The correct answer is a. Traits that help individuals survive and reproduce become more common in the population over successive generations. This process is known as natural selection.
Other Questions
The binary value 1111 0110 in 2's complement equals __________in base 10.a) 10b) Ac) F6d) -6 1. There are 5 red , 7 blue and 6 green biros in a packet. What is probability of a boy picking a green or red biro closing his eyes ?2. In a basket of fruits, there are 6 grapes , 11 bananas and 13 oranges. if one fruit is chosen at random , what is the probability that the fruit is either a grape or banana ? For this week's discussion, answer the following questions: 1. Explain the income measurement process in a merchandising company 2. A company has excess inventory and decides to use it to meet its annual sales goal. It starts to ship orders to customers although the merchandise has not been requested or a purchase order submitted. The justification for doing this is that the company accepts returns and customers can always send the goods back the following period. What are the accounting implications of the company's action? Note: The answer must be based on accounting rules or principles. 3. What factors does a company that lowers the price of its products in order to sell more units need to consider? Which two pieces of information would most likely come from a secondary source about a novel?summary of an incident from the novel being analyzedbackground information about outside influences on the author of the novelinteresting facts about the background of the novela direct quotation from the novel being analyzed An infant with a total blood volume of 800 mL would start showing signs of shock when as little as ______ of blood is lost. Group of answer choices describe the mechanism of fluorescence. in what respects is a fluorescence spectrum not the exact mirror image of the corresponding absorption spectrum? what is the percentage of hydrogen in sulphuric acid? What potential difference must be applied to an x-ray tube to produce x-rays with a minimum wavelength of 45.0 pm? Letter to a friend telling him or her about your church Author Study: HomerWhy do some scholars assert that the poems were composed in the sixthcentury B.C.Ea. This is based on the most current archaeological evidence.b. Because the setting of the Iliad and the Odyssey are the Greek Bronze Age.c. This date validates the historical authenticity of the poems.d. This is when the first written versions of the poems appeared. How do you prove the equilateral triangle theorem? which of the following are requirements for a probability distribution? which of the following are requirements for a probability distribution? a. numeric variable whose values correspond to a probability.b. the sum of all probabilities equal 1. c. each probability value falls between 0 and 1. d. each value of random variable x must have the same probability. Can someone please help with this one? Thank you! Complete the sentence with the best relative pronoun.A fossil ................ archaeologists uncovered in Germany provides evidence that birds were capable of spreading pollen to plants at least forty-seven million years ago. What does negative 5 over 2 < 1 indicate about the positions of negative 5 over 2 and 1 on the number line? (1 point)A. negative 5 over 2 is located on the left of 0 and 1 is located on the right of 0B. negative 5 over 2 is located on the right of 0 and 1 is located on the left of 0C. negative 5 over 2 is located on the left of 1D. negative 5 over 2 is located on the right of 1 Isotopes are:A. found in the nuclear reactions in stars but not on Earth.B. are only theoretical.C. only formed in laboratories.D. found in nature. please help witb the wuestion. thank you.Discuss the different mechanisms by which enzyme activity can be regulated in a cell, also explain why regulation of enzyme activity is crucial to sustain cellular life. Question 3 (12 points) Complete each sentence about the three main philosophies of ancient China: Confucianism was founded by Confucius and is focused on li or proper behavior where everyone follows the responsibilties of the five basic relationships (i.e. ruler and subject); this philosophy became especially popular during the Han dynasty. and following the Dao will allow people to achieve and the idea that rulers to have fewer laws in Daoism was founded by Han Laozi who believed having power was unnatural happiness by harmonizing through nature. Central to Daoism is the yin and yang place. Legalism was founded by Han Fei who wanted to make China united and powerful laws and that rulers should not trust strict anyone. Word Bank: sage strict trust Confucius behavior yang subject Han Han Fei again. He believed that there should be many unnatural fewer powerful Suppose f(x, y, z) = xyz3 +e(y=x2) (a) At the point (-1,1,2), find the direction in which the maximum rate of change of f(x,y,z) occurs. (b) What is the maximum rate of change of the function at the point (-1,1,2)? Enter your answer in the blank blow. Round your answer to two decimal places. Which of the following authentication methods is 100 percent accurate?a. Smart cardb. Fingerprint authenticationc. User IDd. None of the above