briefly explain how an endonuclease (like cas9) compares to a restriction enzyme (like haeiii or bcli). what is a similarity? what is a difference?

Answers

Answer 1

In comparison , Cas9 is an endonuclease and is developed as an immunity mechanism against viruses, which  are not considered restriction enzymes.

Talking about the similariites and differences, endonuclease is an enzyme by cleaving the internal covalent bonds linking nucleotides to break down a nucleotide chain into shorter chains, whereas the restriction enzyme, considered the restriction endonuclease can cleave DNA molecules into small pieces. Cas9 is an endo-deoxyribonuclease, it trims the DNA a little bit, from the cut site, one of the differences is that to identify the target sites in the DNA restriction enzymes doesn't have an RNA or a DNA as a tether. They can recognize the restriction sites by themselves through their DNA binding domains. Compare to restriction enzymes, the endonuclease can't cleave any DNA complementary to the guide RNA, they need the help of protospacer adjacent motifs adjacent sequences to the target site. The cleaving process occurs at a specific site called the restriction site, of the  DNA molecule,  where the site is composed of  4 to 8 base pairs. The restriction enzyme is all about all in one system whereas the endonuclease enzyme recognizes first and cleaves the latter, within a  specific nucleotide sequence.

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Related Questions

What do magnetic lines indicate?

Answers

the direction in which the magnetic force is acting at any particular point.

1. . What is facilitated diffusion?


2. Name one type of proteins are involved in facilitated diffusion?


3. True / False: Diffusion of water and facilitated diffusion require energy from a cell.

4. Diffusion of water and facilitated diffusion are forms of
transport.
__________________



5. What is osmosis?


6. Controlling osmosis is important in helping a cell maintain
which is a stable internal environment.
_____________________


7. In osmosis, water molecules move toward the region of
concentration.
___________________


8. Osmosis works because water molecules (solvent) can move through a membrane, but __________________________ cannot.

Numbers 9 and 10 are on the next page.

9. Two solutions that have equal concentrations are said to be ________________.

10. If a red blood cell is placed into a hypertonic solution, what will happen to that red blood cell?

Answers

Facilitated diffusion is a type of passive transport of molecules across the plasma membrane.

What is facilitated diffusion?

1. Facilitated diffusion is a passive transport of molecules, which means that it does not spend energy to be able to generate the passage through the membrane. So the molecule is going to be passing down its concentration gradient.

2. One class of proteins involved in facilitated diffusion is carrier proteins.

3. False: Diffusion of water and facilitated diffusion does not require energy expenditure.

4. Diffusion of water and facilitated diffusion are forms of passive transport.

5. Osmosis is a process in which there is a movement of water across a semi-permeable membrane.

6. Controlling osmosis is important in helping a cell maintain which is a stable internal environment it's true.

7. In osmosis, water molecules move toward the region of higher concentration of molecules.

8. Osmosis works because water molecules (solvent) can move through a membrane, but solutes cannot.

9. Two solutions that have equal concentrations are said to be isoosmotic.

10. If a red blood cell is placed into a hypertonic solution, the cell will shrink as the water will leave it in order to equalize the concentrations.

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How did the ancestors of NWMs get to South America from their genetic ancestral "home" in Africa?
a) By crossing a land bridge between continents
b) Through a series of migrations by sea
c) By following migratory patterns of their prey
d) Through human intervention and transportation

Answers

The ancestors of NWMs (New World monkeys) got to South America from their genetic ancestral "home" in Africa through the following means: By crossing a land bridge between continents.Option (a) is correct.

What are New World Monkeys?New World monkeys are native to Central and South America, and are characterized by their flattened noses, prehensile tails, and a broad range of physical sizes. They are very much distinct from Old World monkeys, who are native to Africa and Asia.What was the significance of the land bridge between South America and Africa?

A land bridge that connected South America and Africa existed around 30-40 million years ago. The animals, including primates, were free to migrate back and forth between the two continents, and this migration route may have been used by New World monkeys' ancestors to travel to South America and colonize it.Thus, the ancestors of NWMs got to South America from their genetic ancestral "home" in Africa by crossing a land bridge between continents.

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Where does photosynthesis happen the fastest and the slowest?

Answers

Answer: It's basically fastest when white light hits and when yellow, red and green light hits it's at its lowest

Explanation: i hope this helped

list two examples of biotechnology that involve recombinant dna technology

Answers

Recombinant DNA technology is a technique that involves joining DNA molecules from two different sources, and is commonly used in biotechnology.

Two examples of biotechnology that involve recombinant DNA technology are:1. Genetic engineering of crops:In agriculture, recombinant DNA technology has been used to create genetically modified crops that are resistant to pests and diseases, as well as to produce higher yields. For example, scientists have used recombinant DNA technology to insert genes into crops that make them resistant to herbicides or to produce higher levels of vitamin A.2. Production of pharmaceuticals:Recombinant DNA technology is also used to produce pharmaceuticals, such as insulin. Scientists can use recombinant DNA technology to produce human insulin in bacteria, which can then be harvested and used to treat diabetes. Other pharmaceuticals produced using recombinant DNA technology include vaccines, growth hormone, and clotting factors.

Genetic engineering of crops:In agriculture, recombinant DNA technology has been used to create genetically modified crops that are resistant to pests and diseases, as well as to produce higher yields.

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Which of the following is not a method by which antibiotics attempt to kill bacteria?
A Crippling the cell wall
B Forming a membrane around the cell
C Interfering with protein formation
D Block RNA and DNA synthesis

Answers

Answer:

(D) Block RNA and DNA synthesis.

Explanation:

Antibiotics conquer foreign bodies and germs through the process of Phagocytosis, nicknamed cell eating. this basically a process involving the surrounding of the invading cell and pentrating through the Cell wall and thus weakening the cell's overall structure.

It is possible to discover a fully preserved organism? Explain?

Answers

Answer:

No.

Explanation:

No, It is not possible to discover a fully preserved organism because after the death of organism, the decomposers feed on the animal and only the skeleton remains from decomposers. With the passage of millions of years, the fossil is not remain fully preserved due to the environmental conditions so that's why it is concluded that it is impossible to discover a fully preserved organism.

whats a wave in science terms

Answers

Answer:

Definition of a Wave

Explanation:

a disturbance or variation that transfers energy progressively from point to point in a medium and that may take the form of an elastic deformation or of a variation of pressure, electric or magnetic intensity, electric potential, or temperature.

Which claim is supported by the information in Graph 1 and Graph 2 ?

Answers

Answer:

please where is the graph

hassan's dad has blood type b and hassan has blood type ab. what are the possible genotypes of hassan's mother's blood?

Answers

From these Punnett squares, we can see that if Hassan's father is BO, then there is a 50% chance that his mother has blood type A (AO genotype) and a 50% chance that she has blood type O (OO genotype).

If Hassan's father is BB, then his mother must have blood type A (AO genotype) in order for Hassan to have blood type AB.

For Hassan's father, we know that he has blood type B, which means he has the genotype BB or BO (where O represents the absence of the B antigen).

For Hassan, we know that he has blood type AB, which means he has one allele for A and one allele for B. Therefore, his genotype must be AB.

To determine the possible genotypes of Hassan's mother, we can use a Punnett square.

If Hassan's father is BO, the Punnett square would look like this:

B O

B BB BO

A AB AO

If Hassan's father is BB, the Punnett square would look like this:

B B

A AB AB

O BO BO

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The possible genotypes of Hassan's mother's blood are B and O.

Since Hassan's father has blood type B, he can pass on either a B or an O allele to his child. Since Hassan has blood type AB, he must have inherited an A allele from his mother. This means that Hassan's mother could have a genotype of either BO or BB, with the former being more likely since it would result in the AB blood type of their child. If Hassan's mother had a BB genotype, then Hassan would have inherited a B allele from his father and an A allele from his mother, resulting in blood type AB.

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why goosebums appears in human body?​

Answers

it’s a reaction the body has when it’s cold

Explanation:

When you're cold, or you experience a strong emotion, such as fear, shock, anxiety, sexual arousal or even inspiration, goosebumps can suddenly pop up all over the skin. They occur when the tiny muscle located at the base of each hair follicle contracts.

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The human population is increasing every day. How does this increase in population affect the natural resources that maintain humans?

a) Increases the supply of resources
b) Decreases the amount of resources
c) Results in less demand for resources
d) Improves the quality of the resources

Answers

The natural resources that maintain humans results in decreases the amount of resources. The correct option is b.

What are natural resources?

Natural resources are resources derived from nature and used with minimal modification. This includes commercial and industrial use, aesthetic value, scientific interest, and cultural value.

The environmental impact of so many people is manifested in two ways: consumption of resources such as land, food, water, air, fossil fuels, and minerals.

Consumption waste products include air and water pollutants, toxic materials, and greenhouse gases.

Thus, the correct option is b.

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The input of sewage, fertilizer runoff, and animal waste can cause the formation of Choose one: A. an algal bloom. B. nutrient-poor soil C. fertile farmland. D. an artesian well.

Answers

Answer:

An algal bloom.

Explanation:

The input of sewage, fertilizer runoff, and animal waste can cause the formation of an algal bloom (abundances of cyanobacteria)  because of the excess nutrients (nitrogen or phosphorus). If there are too much nutrients in the water (caused by the sewage, fertilizer runoff, and animal waste) it creates a perfect environment for excess growth, being an algal bloom. Algal blooms are toxic and are known to be harmful to the animals that rely on this source for their survival and humans as well.

It is the often the brightly colored, fragrant, and showy part of the flower that attracts the birds and insects that pollinate them and a surface for the pollinator to rest on.

Answers

petals attract birds and insects that pollinate them

Answer:

petals

Explanation:

ye

I need desperate help.​

I need desperate help.

Answers

the answer is b, numerous meaning more in amount

Answer:

Fish make up the largest group of vertebrates.

Explanation:

"Numerous" means a count of something. The second option is the only one that mentions a count of something so that is most likely the answer.

the parietal lobes are to ________ as the occipital lobes are to ________.

Answers

The parietal lobes are to sensory processing as the occipital lobes are to vision.

The parietal lobes are located at the top of the brain and are responsible for processing sensory information from the body, such as touch, temperature, and pain. The occipital lobes, located at the back of the brain, are responsible for vision. The occipital lobes are the main region of the brain responsible for processing visual information from the eyes.

The parietal lobes and the occipital lobes work together to form a complete sensory experience. The parietal lobes process the tactile information from the body and then send this information to the occipital lobes for visual processing. This information is then integrated in the brain to form a complete sensory experience.

The parietal lobes are also important for spatial awareness and navigation. The parietal lobe helps the brain to form a spatial map of the environment, allowing us to identify objects, remember directions, and navigate our way around. This is an important ability that helps us to move around and interact with the world.

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What is a centromere

Answers

The region of a chromosome to which the microtubules of the spindle attack, via the kinetochore during cell division

PLEASE ANSWER LOLL 100 points

PLEASE ANSWER LOLL 100 points

Answers

Answer:

\(\textsf {Option 2}\)

Explanation:

Hot springs and geysers are formed by the heating of groundwater. This is evidenced by the bursting of these natural phenomena, releasing heat.

Chromosomes are pulled apart by the spindle fibers during

Answers

Answer:

Chromosomes are pulled apart by the spindle fibers during Anaphase 1

Explanation:

In anaphase I, the attachment of the spindle fibers is complete. The homologous chromosomes are pulled apart and move towards opposite ends of the cell.

Greenhouse gases are essential to support life on earth. Please select the best answer from the choices provided T F

Answers

The answer is true the earth needs green house gases

Answer:

true

Explanation:

Observable traits exhibited by an organism do not always indicate the exact genetic makeup of that organism . Which explains this ?

Answers

Answer:Any organism is a by-product of both its genetic makeup and the environment. ... In biology, a gene is a section of DNA that encodes a trait. ... An autosomal dominant allele will always be preferentially expressed over a recessive allele. ... Whilst a phenotype is influenced the genotype, genotype does not ...

Explanation:

Answer:

Im pretty sue its b

Explanation:

______________ memory is our memory for facts and knowledge.

Answers

Semantic memory is our memory for facts and knowledge. Semantic memory refers to our long-term memory system responsible for storing general knowledge and factual information about the world & language.

It encompasses our understanding of words, meanings, ideas, and the relationships between them. Semantic memory allows us to recall facts and information without relying on specific personal experiences associated with them. For example, knowing that Paris is the capital of France or that water boils at 100 degrees Celsius are instances of semantic memory.

This type of memory is distinct from episodic memory, which relates to our personal experiences and events. Semantic memory is essential for cognitive processes such as problem-solving, decision-making, comprehension, and communication. It forms the basis of our general knowledge and plays a fundamental role in various aspects of our daily lives.

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what is the role of a promoter region of a gene? what is the role of a promoter region of a gene? serve as the original region of transcription of a gene. recruit rna polymerase to the transcriptional start site. recruit transcription factors that form the initiation complex. recruit rho protein to assist in transcription. protect the gene from mutations in intergenic regions.

Answers

Answer:

According to genomics, a gene's promoter is the area of DNA upstream of it where important proteins, such RNA polymerase and transcription factors, bind to start the gene's transcription. A molecule of RNA is created as a result of the transcription (such as mRNA).

Explanation:

Function of  promoter region  of a Gene :To ensure that you obtain strong and robust transcription of that gene, promoters can be highly complicated and collaborate with other DNA regions known as enhancers. This is a field that has grown significantly and contributed to our understanding of a wide range of disorders.

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According to genomics, a gene's promoter is the area of DNA upstream of it where important proteins, such RNA polymerase and transcription factors, bind to start the gene's transcription.

What is promoter?

An area of DNA known as a promoter is where RNA polymerase starts to transcribe a gene. Promoter sequences are often found upstream of the transcription initiation site or at its 5′ terminus.

RNA polymerase and the required transcription factors bind to both promoters and transcription initiation sites. Promoter sequences identify the DNA strand that will be transcribed and describe the direction of transcription.

Promoters use the information RNA polymerase provides to them in these two steps to determine how lactase will be produced. Promoters can range in length from 100 to 1,000 base pairs.

Therefore, According to genomics, a gene's promoter is the area of DNA upstream of it where important proteins, such RNA polymerase and transcription factors, bind to start the gene's transcription.

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Which statement is true about the differences between plant and animal cells? A) Only plant cells have cytoplasm. Reactivate B) Animal cells are always larger than plant cells. Reactivate C) Plant cells have vacuoles, but animal cells do not. Reactivate D) Only plant cells have chloroplasts that help make food during photosynthesis.

Answers

Answer:

D. Only Plant Cells have Chloroplasts that help make food during photosynthesis.

Explanation:

I always think of it like this-

Plants are green...What make them green? Chloroplasts

Animal cells are not green because they do not have chloroplasts.

A cannot be the answer because animal cells also have cytoplasm that suspend organelles.

B cannot be the answer because all cells come in a range of sizes and some plant cells can be bigger.

C cannot be the answer because some animal cells can have vacuoles.

Answer:

d

Explanation:

took the usatestprep

What green algal group are you not likely to find in fresh water? Ulvophyceae Charophyceae Chlorophyceae Embryophyccae Which of these is not a shared, derived characteristic (synapomorphy) of land plants (Embryophytes), chlorophyll b antheridia and archegonia with sterie jacket layers multicololar embryos ability to make sporopolenha-walled spores.

Answers

The group of green algae that are not likely to be found in fresh water is Charophyceae.

Charophyceae, a class of green algae, are mostly found in freshwater habitats but are also found in soil or other damp places. A distinct feature of Charophyceae is their sporophyte with foot, seta, and capsule. There are multicellular sex organs, antheridia and archegonia with a sterile jacket layer, which is not present in other green algae.

Land plants (Embryophytes) have many characteristics that they do not share with green algae. Chlorophyll b is a shared, derived characteristic of land plants (Embryophytes), while multilayered embryos, sporopollenin-walled spores, and gametangia with a sterile jacket layer are also unique to them.

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What will happen if the enzyme is added to proteins? Explain

Answers

If the enzyme is added to proteins, it will catalyze a reaction that will break down the proteins into smaller molecules, such as amino acids. This process, known as hydrolysis, is necessary for the body to absorb proteins and use them for energy and other metabolic processes.

pls i want help on this sum

pls i want help on this sum

Answers

━━━━━━━☆☆━━━━━━━

▹ Answer

a. $103.50

b. £4400

▹ Step-by-Step Explanation

$90 * 0.15 = $13.50

$90 + $13.50 = $103.50

£5000 x 0.12 = £600

£5000 - £600 = £4400

Hope this helps!

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Brainliest is greatly appreciated!

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Magnetic fields on the sun are produced by the sun’s uneven rotation and:
A. The movement of sunspots on the sun’s surface.
B. The movement of gases in the corona.
C. Gases in the convective zone.
D. Gases in the radiation zone.

Answers

Answer:

The correct answer is - C. Gases in the convective zone.

Explanation:

The heat of the sun causes the atoms to be ionized and create a charged gas known as plasma. These gases or plasma are move in the convective zone with the uneven and rapid rotation of the sun.

This creates the electric currents due to the charge in gases which ultimately create magnetic fields. Sun's rotation causes the sun's overall magnetic field to have some degree of order and to be somewhat stable over time.

you now know that inheritance of eye color in fruit flies is sex-linked: the gene encoding eye color is located on the x chromosome, and there is no corresponding gene on the y chromosome. how would the inheritance pattern differ if the gene for eye color were instead located on an autosome (a non-sex chromosome)? recall that for autosomes, both chromosomes of a homologous pair carry the same genes in the same locations.

Answers

If eye color exhibits autosomal (non-sex chromosome) inheritance. Then, in this case we have 100% red eyes and 0% white.

Let's suppose a cross between large number of white-eyed females with red-eyed males. Then considering two separate cases:

Case 1: Eye color exhibits sex-linked inheritance Case 2: Eye color exhibits autosomal (non-sex linked) inheritance. (In this case, assume that the red-eyed males are homozygous )

For each of this case, predict that how many of the male and female offspring would have red eyes and white eyes.

In Case 1: Eye color exhibits sex-linked inheritance If there were 100 female offspring would have red eyes and zero would have white eyes. If there were 100 male offspring zero would have red eyes and 100 would have white eyes.

And in Case 2: Eye color exhibits autosomal (non-sex-linked) inheritance. If there were 100 female offspring, 100 would have red eyes and zero would have white eyes. If there were 100 male offspring 100 would have red eyes and zero would have white eyes.

So, in this case we have 100% red eyes and 0% white.

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Individuals do not evolve. The smallest ecological unit in which evolution can occur is the population. true or false

Answers

True. Individuals do not evolve. Evolution is a change in the genetic makeup of a population over time.

It occurs due to various factors such as genetic mutation, genetic drift, natural selection, gene flow, and non-random mating. Individuals do not evolve because they do not undergo changes in their genetic makeup during their lifetime. It is the frequency of alleles or genes within a population that changes, leading to the evolution of the population as a whole. Therefore, evolution occurs at the level of populations, not individuals. A genetic mutation is a change in the DNA sequence that makes up a gene. Mutations can occur spontaneously or be caused by external factors such as exposure to radiation, chemicals, or certain viruses.

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Why did the US issue the Chinease Exclusion Act? A 95 percent confidence interval for the proportion difference p1p2 was calculated to be (0. 12,0. 17). Which of the following conclusions is supported by the interval? Indicate whether each statement describes a multiple-step income statement or a single-step income statement 1. Does not separately report income from operations 2 Does not separate revenues and gains into operating or nonoperating 3. Separates revenues and gains into operating or nonoperating Does not separate expenses and losses into operating or nonoperating Single-step income statement Single-step income statement Multiple-step income statement Single-step income statement U 4 Is (10,2) a solution to the equation 3y+14=2x? What adaptation has arisen in sap-feeding Hemiptera to deal with dilute diets?A long stomachA filter chamberLarge mandiblesSalivary secretions QUESTION 2 The following is a Time Series of Two Years (2020-2021) Year Sales 139 398 2020 228 Seasons Q1 Q2 Q3 Q4 Q1 Q2 Q3 Q4 260 121 321 2021 245 280 Find the Centered Moving Average for Q4- 2020 (Round your answer to 2 decimal places) OUESTION 3 QUESTION 2 The following is a Time Series of Two Years (2020-2021) Year Sales 139 398 2020 228 Seasons Q1 Q2 Q3 Q4 Q1 Q2 Q3 Q4 260 121 321 2021 245 280 Find the Centered Moving Average for Q4- 2020 (Round your answer to 2 decimal places) OUESTION 3 En la historia del origen del dinero es necesario mencionar la historia de la banca, ya que fueron los primeros en custodiar las monedas de las personas fueron los: If you want states to keep the most important powers are you a Federalist or an Anti-Federalist? place answer need answered nowww!! 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